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Invisiblewisdom187
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Registered: 10/12/10
Posts: 301
Suggestion of what i should do with colonized agar
    #14158834 - 03/21/11 11:23 AM (12 years, 10 months ago)

i cloned a tissue on a plate of agar, its not divided into 3 parts its just one agar section, and now its fully colonized out to the edges.


should i take that whole chunk and drop it in a grain jar then G2G that? or should i cut out 5-6 pieces and drop them into grain jars?


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Invisiblehiggledy-piggledy

Registered: 08/24/10
Posts: 966
Re: Suggestion of what i should do with colonized agar [Re: wisdom187]
    #14158882 - 03/21/11 11:37 AM (12 years, 10 months ago)

one piece per jar is enough. and then grain 2 grain that if you want.

cut out another little piece and put it on a second plate and try to isolate (if not already one strain).

store the rest of the plate in the fridge.


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InvisibleDoc_T
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Re: Suggestion of what i should do with colonized agar [Re: wisdom187]
    #14158889 - 03/21/11 11:39 AM (12 years, 10 months ago)

I'd take out several small sections, drop them into half pint or pint grain jars.
When those colonize, first make grain lc and then use them as grain 2 grain masters.

Two or three separate wedges cut from one triangle, about the size of a dime total are, or a little larger, per jar.
Then seal up the petri in a ziploc bag and refrigerate.

Store the grain lc until you see how the culture fruits.
If you like it, a few drops of glc inoculates the grain masters for your next round, and one syringe become a hundred tubs or more.


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OfflineRogerRabbitM
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Re: Suggestion of what i should do with colonized agar [Re: wisdom187]
    #14158909 - 03/21/11 11:44 AM (12 years, 10 months ago)

Quite frankly, I wouldn't use it to inoculate grains.  If you're cloning, you should only let the mycelium grow out 1" or so maximum before grabbing a tiny piece of mycelium from the leading edge of the growth, and transfer it to a new plate.  This ensures you've isolated away from molds or bacteria that were left on the original tissue.

You can take a tiny piece now and transfer to a fresh dish, but I'd repeat the above once more before going to grains.
RR


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InvisibleTheShroomJew23
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Registered: 11/29/07
Posts: 1,611
Loc: Temple
Re: Suggestion of what i should do with colonized agar [Re: RogerRabbit]
    #14158956 - 03/21/11 11:58 AM (12 years, 10 months ago)

I took a plate of an oyster isolate, one wedge went to an LC, one wedge went to a grain master, and the last wedge went to several slants. That is of course when the mycelium was only about an inch out. I now have 25 4-pound spawn bags, 15 spawn jars, and a ton of master grain/lc. Utilize your mycelium to maximize its biological efficiency.


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