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OfflineBlueflowers
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LC = easier contams
    #14158387 - 03/21/11 09:02 AM (12 years, 10 months ago)

Hey guys im just wondering whether i should go straight out spore syringe or try LC method , the reason for this is because i only have 1 spore print and i want to innoc atleast 5 quart rye jars. though i have herd Liquid cultures are alot more susceptible to contams?

thanks

BF


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Invisiblewow
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Re: LC = easier contams [Re: Blueflowers]
    #14158396 - 03/21/11 09:06 AM (12 years, 10 months ago)

if i were you id make a spore syringe and knock a few rye and a couple lc's..  when you have lc use it to see if its good...  or g2g one of the successful jars if it works out for ya..


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InvisibleGerman Kahuna
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Re: LC = easier contams [Re: Blueflowers]
    #14158416 - 03/21/11 09:11 AM (12 years, 10 months ago)

If all you got is a print then don't do it. If you stop and think for a moment then you'll find out why all by yourself.
A spore print is never completely clean. Unlike on agar it is impossible to tell what you are actually growing in an LC. That's why you only start an LC from something you know to be clean (e.g. an agar wedge from a clean culture). Spores from a print should always be started on agar so you can spot contaminations and transfer clean mycelium away to a fresh dish.
There'll be others that say that they have gone straight from print to LC and it worked just fine. Maybe. But it's taking chances and not professional procedure, IMHO.
Also I believe you don't really have an idea just how many spores one print is. You can make like 10 10ml syringes from a dark average sized print. That will noc up 100 jars if you want.


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InvisibleDoc_T
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Re: LC = easier contams [Re: German Kahuna]
    #14158451 - 03/21/11 09:23 AM (12 years, 10 months ago)

Agreed w/GK. Plenty of spores in a print, you can try an LC jar if you like.
But for the time and money involved you'd be better off with grain lc or agar. Something you know will work.


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InvisibleTranscendingLife
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Re: LC = easier contams [Re: Doc_T]
    #14158527 - 03/21/11 09:45 AM (12 years, 10 months ago)

Quote:

Doc_T said:
Agreed w/GK. Plenty of spores in a print, you can try an LC jar if you like.
But for the time and money involved you'd be better off with grain lc or agar. Something you know will work.




:whathesaid: I'd recommend GLC & some G2Gs...Both guarantee, as long as you're sterile during the procedures, clean myc to grow.

Personally, I haven't touched a MS spore syringe in over 2 months, because I've been doing so many GLCs & G2Gs. They're both excellent ways to ensure a clean culture w/ MS & extend the life of 1 jar/syringe/print.

All that being said, GK is completely right. Iso work on agar is the hands down best way to go. However, being new to the hobby, I understand why you would be somewhat intimidated by the idea. I know  I was.

Just take it slow, do your research, use the search function, ask questions & you'll be rockin' in no  time.

GL


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OfflineTheIndoCloud
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Re: LC = easier contams [Re: TranscendingLife]
    #14158718 - 03/21/11 10:46 AM (12 years, 10 months ago)

Grain LC all the way! Its pretty much the best way to make an LC.


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InvisibleTranscendingLife
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Re: LC = easier contams [Re: TheIndoCloud]
    #14158725 - 03/21/11 10:49 AM (12 years, 10 months ago)

Quote:

TheIndoCloud said:
Grain LC all the way! Its pretty much the best way to make an LC.




yuppers, what I do now is make 1 sterile water jar for the GLC (like normally you would) + 1 other jar that has normal LC materials in it (like ELME or karo or whatever you use). I do the normal GLC thing then when I'm done liberating the myc, I shoot that into my LC jar. Works like a charm...see


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineTheIndoCloud
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Re: LC = easier contams [Re: TranscendingLife]
    #14158731 - 03/21/11 10:51 AM (12 years, 10 months ago)

Thats... beautiful! Sniffle


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InvisibleStropharis
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Re: LC = easier contams [Re: TheIndoCloud]
    #14158750 - 03/21/11 10:56 AM (12 years, 10 months ago)

It seems that most of the more experienced cultivators do not recommend LC because it is so hard to tell if you are working with contaminants until it is too late. If not working with Agar, I would recommend mycelium laden water which will give you the same results.
Grain To LC


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OfflineBlueflowers
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Re: LC = easier contams [Re: Doc_T]
    #14161891 - 03/21/11 10:24 PM (12 years, 10 months ago)

Mmm i would love try try agar since contams can be easily avoided though like todlow said i am extremely new to this hobby and am a little intimidated by agar culture. i think for now i will go with G2G or maybe even GLC , unless i find a noob friendly agar tek. :wink:

thanks everyone!

BF


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Invisiblebiologys
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Re: LC = easier contams [Re: Blueflowers]
    #14161905 - 03/21/11 10:26 PM (12 years, 10 months ago)

you cant get any simplier then the grocery store agar tek..


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InvisibleTranscendingLife
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Re: LC = easier contams [Re: Blueflowers]
    #14161930 - 03/21/11 10:31 PM (12 years, 10 months ago)

:2cents: The most noob friendly "guaranteed" thing to do is GLC. That's all proper needle sterilization tek. But like I said, that statement is IMHO.

bio, you can :pm: me about it, but I beg to differ. (E)LME+Agar = easiest Agar. :2cents: again (I know)  :rolleyes:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleDoc_T
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Re: LC = easier contams [Re: TranscendingLife]
    #14161947 - 03/21/11 10:33 PM (12 years, 10 months ago)

Agreed w/ both points.

Agar is easier than it seems, but glc is easier still. And MEA is awesome.


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You make it all possible. Doesn't it feel good?


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Invisiblebiologys
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Re: LC = easier contams [Re: Doc_T]
    #14162078 - 03/21/11 10:57 PM (12 years, 10 months ago)

TL, this last batch of agar i did, i just experimented I did 1 tblspoon of ELME to 1 cup of water + half stick of agar...will known in another day or two how it worked lol


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Invisiblewow
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Re: LC = easier contams [Re: biologys]
    #14162254 - 03/21/11 11:31 PM (12 years, 10 months ago)

my ghetto agar is pretty simple!!  i just made a clone on it yesterday and its already growing...  ive been experementing with this agar for a couple weeks and it works to germinate spores and clone mushrooms..  http://www.shroomery.org/forums/showflat.php/Number/14128955/page/1

i could definately do better..  but this stuff works OK!  and its EASY!



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InvisibleDoc_T
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Re: LC = easier contams [Re: wow]
    #14162278 - 03/21/11 11:34 PM (12 years, 10 months ago)

Is that wheat? In a 4oz jar?


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You make it all possible. Doesn't it feel good?


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Invisiblewow
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Re: LC = easier contams [Re: Doc_T]
    #14162346 - 03/21/11 11:46 PM (12 years, 10 months ago)

na :lol:  its a microwaved whole rice cake!!  this is my second try and so far so good!  check this thread for the details on this experiment!
http://www.shroomery.org/forums/showflat.php/Number/14049567/fpart/1

the agar..  im about to make a transfer tonight..  its got green spots because i didnt use a gb but now i made one!


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OfflineBlueflowers
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Re: LC = easier contams [Re: Doc_T]
    #14163188 - 03/22/11 05:31 AM (12 years, 10 months ago)

Hmmm well GLC or agar that is the question.......:confused: ahh can't decide!!


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Invisiblewow
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Re: LC = easier contams [Re: Blueflowers]
    #14163653 - 03/22/11 09:36 AM (12 years, 10 months ago)

if you got agar...  its the way of the tribe....  glc works nice i hear..  but you will save spores for sure using agar..  you need a GB tho because agar will grow everything that lands on it..


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