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Offlinedr harry bush
Grow wiz kid
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Registered: 03/19/11
Posts: 322
Loc: 3rd rock from the sun
Last seen: 11 years, 7 months
Erythroxylum Novogranatense and peyote tissue culture.
    #14157682 - 03/21/11 02:18 AM (12 years, 10 months ago)

good day all i might need some help to complete this i will need to know what part of the plant do i have to get the tissue sample from ?

there is how i made up my media mix, i have use pretty much the same mix with orchids and ice plants.

per 500ml water 1gram of nutrient powder was added, i then added 0.20 grams hormone, the added 5grams glucose and 5grams sucrose, 12 grams of agar was then added and the mix was pc ed at 15 psi for 30 min.



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OfflineLC89
Shamn Intern
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Registered: 08/21/10
Posts: 261
Loc: San Diego CA
Last seen: 3 hours, 33 minutes
Re: Erythroxylum Novogranatense and peyote tissue culture. [Re: dr harry bush]
    #14157781 - 03/21/11 02:49 AM (12 years, 10 months ago)

u had already done tissue culture before? I know cytokinin increase cells division. Usually you use meristem the active growth point for tissue culture.


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Looking gourmet mushroom/cube


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Offlinedr harry bush
Grow wiz kid
 User Gallery

Registered: 03/19/11
Posts: 322
Loc: 3rd rock from the sun
Last seen: 11 years, 7 months
Re: Erythroxylum Novogranatense and peyote tissue culture. [Re: LC89]
    #14157833 - 03/21/11 03:12 AM (12 years, 10 months ago)

yes i have, work with both the ice plant and orchid.
the only plants still alive though are the ice plants.

thanks for the info, we have limited resources here, i have heard coconut milk is a great source too.


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