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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14156482 - 03/20/11 09:48 PM (12 years, 11 months ago)

I think op is using BRF instead of agar. Just BRF and water, or BRF, Verm, and water. No agar. Just thick rice flour mixture in a thin layer.

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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14156526 - 03/20/11 09:55 PM (12 years, 11 months ago)

see, that was why i asked alil while ago, cause i was confused...why not use agar is super cheap and easier then brf paste to sterilize lol

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14156530 - 03/20/11 09:56 PM (12 years, 11 months ago)

No sorry if it wasn't clear but I am just using BRF and water like I said
Quote:

freeskierpj said:
Yea I'm following this tek HERE




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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14156546 - 03/20/11 09:58 PM (12 years, 11 months ago)

Quote:

biologys said:
see, that was why i asked alil while ago, cause i was confused...why not use agar is super cheap and easier then brf paste to sterilize lol




Without a pressure cooker?


--------------------

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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14156573 - 03/20/11 10:01 PM (12 years, 11 months ago)

I dont see why not, its basicly a liquid, so as long as you get the temp up and keep them for 60-90 minutes it should sterilize it..

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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14156626 - 03/20/11 10:08 PM (12 years, 11 months ago)

I just tried just that. I cleaned a pyrex measuring alcohol w/ IPA, and microwatved the water, added premade LMA powder, and nuked to boiling again. Then I poured into sterile petris opening just enough to quickly pour some in.
I figure if they are still clean in a few days then Ill be fine.
We'll see! Hopefully it works as Its very hard to find agar here and  expensive online. Wish I could find a good used PC cheap!

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14156734 - 03/20/11 10:24 PM (12 years, 11 months ago)

I hope it does work as I'd love to do some agar work!


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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14156826 - 03/20/11 10:35 PM (12 years, 11 months ago)

I am guessing it will work, but I bet Ill have a much higher initial contam rate

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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14156833 - 03/20/11 10:37 PM (12 years, 11 months ago)

That was weird. Ive never been on during the 4 am back-up before!

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14157005 - 03/20/11 11:07 PM (12 years, 11 months ago)

Lol oh I hate the 4 am back up...mine comes at 6am and when I end up on an adderall binge that's my "Oh shit I spent alllllllllll night on the shroomery" wake up call hahahaha...


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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14158162 - 03/21/11 07:00 AM (12 years, 11 months ago)

Quote:

bateyes88 said: Its very hard to find agar here. Wish I could find a good used PC cheap!




Where are you?

Also, thrift stores have PCs.


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OfflineProfessorPinHead
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14158180 - 03/21/11 07:13 AM (12 years, 11 months ago)

Quote:

freeskierpj said:
Lol oh I hate the 4 am back up...mine comes at 6am and when I end up on an adderall binge that's my "Oh shit I spent alllllllllll night on the shroomery" wake up call hahahaha...



expand this thumb nail and read me :smile:

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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: ProfessorPinHead]
    #14158376 - 03/21/11 08:56 AM (12 years, 11 months ago)

i love the back up, i play the game, and always get a high score, but it never saves it :frown:

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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14159285 - 03/21/11 01:24 PM (12 years, 11 months ago)

Now I feel MUCH better, PP. Thanks!lol

Im in East Bumfuct midcoast Maine

Closest agar is Portland 100 miles away. Checked oriental section of "mega-mart" supermarkets. There is like 10 non-whites in the whole state. Even Brown Rice has an inch layer of dust on it.

I should buy a pant-load when I do make it to the city, but it never happens for various reasons. I used the last of my LMA powder last night making 6 dishes w/o PCing em. See how that goes...

Do I need to parafilm the dishes before I nocc em up? Your supposted to keep em upside down for condensatsion, right? Seems like contams can get in the rim if upsidedown and not filmed...

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14159300 - 03/21/11 01:27 PM (12 years, 11 months ago)

Quote:

bateyes88 said: "mega-mart" supermarkets.




You don't have a health food store? Mountain Man's Healthy Co-Op or something?


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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: Doc_T]
    #14159430 - 03/21/11 01:57 PM (12 years, 11 months ago)

There is a members-only one- its very small...I think the public can go, just have to pay extra or somrthing. Not too sure.

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Offlinefaceyneck
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14162426 - 03/22/11 12:00 AM (12 years, 11 months ago)

Quote:

biologys said:
i love the back up, i play the game, and always get a high score, but it never saves it :frown:




how do you know you got a high score? :confused2:

I made it to level 7 once. I think that's my highest. :shrug:


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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: faceyneck]
    #14162442 - 03/22/11 12:04 AM (12 years, 11 months ago)

well, high compared to what i usd to get when i first started lol i can get massive curve ball bonus's haha

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