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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14154090 - 03/20/11 02:08 PM (12 years, 11 months ago)

I have noticed color fade in LCs. One of my favorite LC is diluted apple cider. (Dont laugh! Its AWESOME) It starts out brown, but the myc takes on the brown color and the liquid becomes clear after a week or so.

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154176 - 03/20/11 02:23 PM (12 years, 11 months ago)

yep LC's start out a golden color, and as the mycelium consumes the sugar's ( honey, or LME ) it turns clearer.

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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14154308 - 03/20/11 02:47 PM (12 years, 11 months ago)

I know this is WAY off topic-sorry-but just curious. Does the myc expel waste products as it digests the sugars?

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154333 - 03/20/11 02:54 PM (12 years, 11 months ago)

from my understanding it secrets the myc piss to break things down so it can digest it?

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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14154344 - 03/20/11 02:56 PM (12 years, 11 months ago)

The yellow stuff that pools at the bottom of jars sometimes?

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154353 - 03/20/11 02:57 PM (12 years, 11 months ago)

yes.

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Offline3Cajun1Mo8
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154356 - 03/20/11 02:58 PM (12 years, 11 months ago)

I have tried this with success. I only Steamed for 30 minutes since it wa just a 1/2 inch of BRF in an old olive jar .


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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: 3Cajun1Mo8]
    #14154373 - 03/20/11 03:03 PM (12 years, 11 months ago)

Is it the same ratio as the PF Tek? 2:1:1?

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154413 - 03/20/11 03:09 PM (12 years, 11 months ago)

EDIT: Damn bio you beat me to it! And for the love of god don't call it myc piss!!! Every post I've seen where RR comments on the whole "myc piss=metabolites" he get's upset :lmafo: No but seriously just say metabolites it's not that hard :wink:

Just a very quick search but found THIS:

Quote:

Metabolites are also the natural product of the mycelia's colonization.  The outside surface of the mycelia exudes these chemicals to break down and digest the nutrients/minerals that are made available through this process.  Most mycelia can actually mineralize rock as it secretes oxalic acid.




I'm interested in this as well and although it sounds like he knows what he's talking about I never like to trust just a single source so I'm gonna keep doing some research...I need to get some food coloring though are there any other alternatives to food coloring that may work for this?

Also I might try this but instead of just adding water I would add a solution of water and:
  • Turbinado Sugar
  • Honey
  • Karo


Just to see if the added nutrients would help speed the process up. This is of course once I get my food coloring and make sure that it does actually work and that the myc eats up the BRF agar inside of these.

Oh and one last question, they aren't 100% fully sealed (poor construction of the lids but hey for 99 cents what can you ask for) so would that be a good enough source of GE/FAE or whatever agar needs in order for the spores to germinate?


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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154456 - 03/20/11 03:18 PM (12 years, 11 months ago)

I'd bet coffee wouldn't be bad, either.

So, is this stuff stiff, or really wet, or gluey?


RR def seems to know his stuff. I just saw this quote from him that made me chuckle:

"Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas."
-RR

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14154486 - 03/20/11 03:25 PM (12 years, 11 months ago)

Quote:

3Cajun1Mo8 said:
I have tried this with success. I only Steamed for 30 minutes since it wa just a 1/2 inch of BRF in an old olive jar .






Interesting. Did you use the same tek I was using HERE, and just scale it up? Just as an easy example, 5g brf to 16cc water means 10g of brf to 32cc of water. If not can you give some more insight on your technique?

Quote:

bateyes88 said:
I'd bet coffee wouldn't be bad, either.

So, is this stuff stiff, or really wet, or gluey?


RR def seems to know his stuff. I just saw this quote from him that made me chuckle:

"Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas."
-RR




Good point, this is definitely gonna be something I'll mess around with for a while. And it's more like a gooey slop...I can't really describe the texture too well first off and second I used self ground BRF so it was a little more chunky than if you were to buy BRF from a supermarket...


--------------------

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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14154519 - 03/20/11 03:32 PM (12 years, 11 months ago)

Cool thanks. Thats all I needed. Just wondering if my "glop" was what it is supposed to be lol.
I grind my own, too. Burnt out a couple grinders, but its worth it. Cant beat $.30/lb. And fresh works better, too. Where I live I bet they dont sell but a bag or two a year.

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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14154548 - 03/20/11 03:37 PM (12 years, 11 months ago)

Yeah thats the tek I used, just instead of a 1/2 pint jar I used a Olive jar I had just finished and cleaned with a polyfillid screwed on over tyvek.

I ended up only using the BRF mixture once before i chunked it out, I took a piece out and put it in some Honey water I prepped.  The little chunk of colonized BRF just floated around and mycelium webbed out across the top of the honey water. then nocced up a grain jar  with the floating web and that grain jar is doing fantastic.

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14155192 - 03/20/11 06:08 PM (12 years, 11 months ago)

Quote:

bateyes88 said:
Cool thanks. Thats all I needed. Just wondering if my "glop" was what it is supposed to be lol.
I grind my own, too. Burnt out a couple grinders, but its worth it. Cant beat $.30/lb. And fresh works better, too. Where I live I bet they dont sell but a bag or two a year.



Yea glop is a perfect word to describe it hahaha :laugh: and seems like mine is working! Can't see it well but there is a thin film of mycelium covering the pin here.

And yes PJ is in his PJs...they're comfy as hell!


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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14155346 - 03/20/11 06:38 PM (12 years, 11 months ago)

So, for half pint reg mouth (normal PF Tek jar) 90 minutes is what you came up w/? Is it still "Gloppy" after the 90 min, or does it firm up?

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14155363 - 03/20/11 06:42 PM (12 years, 11 months ago)

wait, so you're just trying to get an agar substitute that you can use with out a PC, or does it have to be BRF?


or are you using BRF and water, with no agar lol im a bit confused.

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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14155451 - 03/20/11 06:57 PM (12 years, 11 months ago)

I think an agar sub for use when a PC is unavailable. At least thats what I need, and I think the OP as well...

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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14155905 - 03/20/11 08:21 PM (12 years, 11 months ago)

Yea I'm following this tek HERE but just added the little twist of doing it in a pill divider after having seen the Pill Divider Agar Tek and for the reason that I have no wide mouthed half pint jars, only regular mouth (not tapered or anything so it still works fine for the PF tek) but it would be a royal PITA to get a little wedge out of there as they are taller and skinnier. I tried using a few other types of containers and still have a few to try out but I wanna be able to tell if my LC's are clean and this seems like the easiest way to do it. Right now I have 3 empty pill containers and the one that I used as a test so that would mean I could check 28 different LC's for their purity and then use those LC's on pasteurized poo/straw/verm/coir (or some variation of that) bags like I was asking about with the hpoo and gypsum question.

I tried doing a pill divider LC tek a few weeks back but it didn't work as the pill dividers I have for some reason aren't 100% air tight so with the BRF agar I covered it in micropore tape around any areas that could let contams in.

Bio - if you have a different agar substitute that doesn't require a PC please fill me in :lol:

Bateyes
- As I stated HERE right after number 3:

Quote:

freeskierpj said:
So after 3 failures;

1) Regular mouth 1/2 pints not the wide mouth plus 90 minutes just made it practically a solid (tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not this way but I would shourten the time for any future experiments.

2) Regular mouth 1/2 pints not the wide mouth plus 60 minutes just made it practically a solid same as 90 minutes (again tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not but I'm gonna shorten the time again...

3) Plastc bead holders (as I had no empty jars and was way high so I figured what the heck, why not. They are stackable and twist into place and had 5 compartments...they weren't PP5 I guess haha even at 45 minutes they were waaaaaaaay bent and shit.

I sat and thought about it and remembered the Pill Divider Agar Tek I used 1cc of water and .3g of BRF per "day", sterilized for 45 minutes again and what do ya know nothing seemed to go wrong! I opened only 1 section up to check consistency and it sounded exactly like that of the BRF Agar Substitute made with a pressure cooker...I let it cool overnight and then inoculated Sunday with ONE DROP of Dancing Tiger MS that had germinated in the mail on the way here, Monday with ONE DROP of x7x that was supposed to be LC but it just looked more like MS solution to me, Tuesday with ONE DROP of GT (practically my last drop so I'm hoping for it to work out), and Wednesday with a tiny pin from my African Transkei BRF jar that FINALLY (7 weeks) colonized fully).

Unfortunately the BRF turns white during sterilization and I can't tell if anything is working out...gonna do another batch with a drop of food coloring (or pigment of some sort, any suggestions?) so that I can actually see the mycelium if this does work.

If so this would be a very easy intro to agar for newbs or for those that have no pressure cooker like myself.




So no 90 minutes caused mine to go solid so I would just mess around with it and try different times but 45 minutes worked for mine.


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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14155996 - 03/20/11 08:35 PM (12 years, 11 months ago)

Right. I wasnt sure if solid was a good thing or not, lol. Ive been reading old posts about it and seems like "rubbery" is what to shoot for.

I know you said "glop", but I thought you meant before steaming so I was unsure what the final product looks like.


One issue that kept coming up is that w/ BRF "agar" is that while it looks OK, there may be contams that wont show up on BRF agar, but will when spawned to grain.
Could just be the normal "shroomery poo-pooers" that crap on ideas that are not thier own. I'm still gonna try it. Different strokes 4 different folks!

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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14156457 - 03/20/11 09:43 PM (12 years, 11 months ago)

so you are actually using AGAR just not pc'n it..

all you need is a nutritional item, so just boil some potatoes, and use the water in your agar mix, or sugar in the raw...etc...and steam it for 60 minutes or 90 mins :shrug: they say grains has to be PC sterilized, but sugar and liquid additives dont..so i'd say sugar, or honey agar should work being steamed..idk

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