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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 10 months
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when is sterilzed syringe ok to use?
#14153301 - 03/20/11 11:26 AM (12 years, 11 months ago) |
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Hey, I gotta pc some old spore syringes today in order to use my LC tomorrow. I pc for 30 minutes usually. When (generally) are they cool enough to suck up LC without killing the myc? Thanks
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: when is sterilzed syringe ok to use? [Re: Bucknaztee]
#14153353 - 03/20/11 11:37 AM (12 years, 11 months ago) |
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Let the PC sit for 6-8 hours to reach ambient room temp. Then you're golden...
Why don't you sterilize by boiling water for 10 minutes then sucking up the water into the syringe then expel the water after letting it sit for 1 minute. Repeat 3-4 times. The last time leave the water in the syringe. I wait about 60 minutes to use it & it's fine.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 10 months
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Yeah bud i thought about the boiling water method; hell i actually clean em that way just before sterilizing. But the idea just seems a bit irresponsible after everything I have learned. I do however, have an abundance of LC, maybe trying a few would put my mind at ease. Though I won't know for sure until fruiting, eh? Thanks again
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: when is sterilized syringe ok to use? [Re: Bucknaztee]
#14153441 - 03/20/11 11:57 AM (12 years, 11 months ago) |
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You'll know if it's contamed w/in the first week...I check my jars 3 days after inoculation then won't look at them for 7 days...after that they're normally ready for a shake, then I check again for contams 3 days later.
GL buddy 
PS: I always use the boiling water method to sterilize my syringes & have yet to have a problem.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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I have never PC'd my syringes, and never once have a had a contam from doing it this way..
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 10 months
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I dig todlow, i dig. I suppose i usually see the green bastard or the cobzweb at about day 3-5. Fuck I got like 50 different jars waiting, i gotta move...thanks again
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: when is sterilized syringe ok to use? [Re: Bucknaztee]
#14153524 - 03/20/11 12:19 PM (12 years, 11 months ago) |
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Quote:
ZLeiterman said: I dig todlow, i dig. I suppose i usually see the green bastard or the cobzweb at about day 3-5. Fuck I got like 50 different jars waiting, i gotta move...thanks again
50 jars! Holy shit brotha...that's a ton...you doing PF tek?
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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