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Invisiblevirus1824
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14182587 - 03/25/11 02:44 PM (12 years, 10 months ago)

extra light and just light doesn't make much difference i reckon. i also add dextrose btw but still no growth on the surface :shrug:


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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: virus1824]
    #14184094 - 03/25/11 09:09 PM (12 years, 10 months ago)

Yeah, I'm makin a batch of 12 8oz BRF jars tonight... giving the LCs a GOOD magnetic stir, and then going to test um out...

I'll definitely post up the results when I start to see some growth in them, whether it be good or bad :P


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Now that's what I call cubes :smile:



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InvisibleDoc_T
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14184283 - 03/25/11 09:53 PM (12 years, 10 months ago)

Test on one jar, not twelve.


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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: Doc_T]
    #14184397 - 03/25/11 10:18 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Test on one jar, not twelve.





well I have 5 different LCs... (4 white islands, 1 other one)

So i figured I would do two jars of each... just 'in-case' one contams due to improper panelization or something, rather than the LC being bad...

plus I'm getting ready to use them for a separate bulk grow... So i wanted to make sure anyways...


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Now that's what I call cubes :smile:



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InvisibleDoc_T
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14184409 - 03/25/11 10:20 PM (12 years, 10 months ago)

Makes sense. Two jars is a better test than one.


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InvisibleFungal growth
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14184417 - 03/25/11 10:21 PM (12 years, 10 months ago)

i'd still only do one jar per lc,
cuz imo those don't look good.
but hopefully i'm wrong.


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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: Fungal growth]
    #14184517 - 03/25/11 10:40 PM (12 years, 10 months ago)

yeah, the hard part tho is going to get it to break up... since the one 'tornado' looking jar is super think... connected to the huge white island on top... so when I turn on my home made magnetic stirrer... it just keeps rotates... not enough force to bring it down to the bottom of the jar and start breaking it up :\




I am tempted tho to screw one jar over, and open it up, just to get a CLEAR shot of the white fuzz, and see what it smells like... very VERY tempted...


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Now that's what I call cubes :smile:



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Offlinefreeskierpj
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14185949 - 03/26/11 09:18 AM (12 years, 10 months ago)

If you need a really big needle to suck that shit up then here's a 14 ga 1.5" luer lock tip


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Invisiblebiologys
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Re: Concern with my Liquid Cultures... [Re: freeskierpj] * 1
    #14185982 - 03/26/11 09:31 AM (12 years, 10 months ago)



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OfflinePerun
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Re: Concern with my Liquid Cultures... [Re: biologys]
    #14186137 - 03/26/11 10:33 AM (12 years, 10 months ago)

Quote:

biologys said:
i use these..works great 4.25 inches long, :super:

http://cgi.ebay.com/10ml-6-injector-LONG-Blunt-Needle-10cc-4-25-/130492881039?pt=LH_DefaultDomain_0&hash=item1e61fb588f





Hehehehehe...I bought the same one from the same guy and they work gr8!!! :laugh:


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First:                                                        Then:


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Invisiblebiologys
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Re: Concern with my Liquid Cultures... [Re: Perun]
    #14186164 - 03/26/11 10:41 AM (12 years, 10 months ago)

i throw the syringes away, love the luer lock but the needles are excellent for LC's


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Invisibleabltsandwich
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14186236 - 03/26/11 11:02 AM (12 years, 10 months ago)

That doesn't look good to me OP but try it out.


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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: abltsandwich]
    #14190136 - 03/27/11 01:35 AM (12 years, 10 months ago)

UPDATE: So i made 10 brf jars to test out the LCs...

Seems to me I'm retarded... cuz if i cant BREAK UP all of whats inside of the LC... then I cant suck it up into a syringe...

No idea how i forgot about that when i decided to make the jars last night... ahg.

So my new plan is to make my grow room into a glove box type setting... aka clean the FUCK outa my walk in closet... and I just made 5 wide mouth plastic lids, drilled one hole in them, and then covered the hole in high-temp sealant... So my goal tomorrow is to undo the lids of my LCs... and then carefully/quickly take off the tyvek and pop on one of the new lids.


This way I can do that to four of them... and then on one of them get a few snap shots of the white fuzz, and maybe a quick smell of it before throwing on the new lid...


and then finally, once the new lids are on... I'm going to shake the FUCK out of these jars... until EVERYTHING is broken down... spore for spore lol


and then suck um up in syringes :smile:


--------------------
Now that's what I call cubes :smile:



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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14192703 - 03/27/11 02:46 PM (12 years, 10 months ago)

Here's the update...

successfully (hopefully!) got the new lids on so I could shake them...

snapped some pictures of the P.E. LC I got goin... might have put it at a huge risk... but figured it was for a good cause and for some good pics... also gave it a quick smell, and it smelled my mushrooms :smile:

so thats a good note...



gotta go to work... shook one of the jars, so compare the results when I get home... for if shaking them contams them... with the new lids on I can now turn them upside down to suck up what i need in a syringe... so I figured I would do some tests before shaking them all...



from the smell alone tho, I think they are still good... I'm hoping I didnt put them at TOO much risk from switching the lids tho, But i feel that my lid-switching-sterelize tek that I came up with last minute was good enough to keep um safe :smile:



I would love some feed back on these pics if ANYONE has seen this before, or knows what causes this ect...



also the jar I shook looks a little bubbly...



hmmm....


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Now that's what I call cubes :smile:



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Invisiblejokefox
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Re: Concern with my Liquid Cultures... [Re: ThisIsPathetic]
    #14200299 - 03/28/11 08:58 PM (12 years, 10 months ago)

mycelium , wow i was right again :datass:


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OfflineThisIsPathetic
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Re: Concern with my Liquid Cultures... [Re: jokefox]
    #14200382 - 03/28/11 09:09 PM (12 years, 10 months ago)

Update... After shaking, it seems that one jar has successfully 'recovered' from the shake... No white islands and it looks pretty healthy (Treasure Coast).

As for the other jars... they seem to be gathering at the top of the jar... while the other half seems to gather at the bottom... and the top of the jar just looks bubbly and almost brown :frown:

I need to go out and get gloves before I can inject the BRF jars i made the other day... Since I had to delay the injection, what do you guys think? Should I re-sterilize the BRF jars? It's going to be almost a full 3 days from when I made them to when I can inject them... buuuut I did put them in an empty CLEAN fish tank... sprayed the entire tank with a decent dose of Lysol, and then just a cover over it...

Still good?

opinions?

remake all 10 test jars?


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Now that's what I call cubes :smile:



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