|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
 Adding penicilin G and streptomycin to agar mix? 
#14148337 - 03/19/11 01:10 PM (13 years, 1 month ago) |
|
|
Hey folks,
Would it be advisable/worthwhile for me to add penicilin G and streptomycin to my agar mixture? I would add the pen and strep after the agar solution cooled a little bit, but before it would begin to solidify. Pen and strep are temperature sensitive, so they would degrade if they were PCed.
What do you guys think? It would, for the most part, eliminate bacterial contamination. Is mold more of a concern than bacteria as far as contaminations with agar are concerned? Srep and pen aren't going to affect myc any, are they?
M
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
|
Doc_T
Random Dude
 
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148347 - 03/19/11 01:13 PM (13 years, 1 month ago) |
|
|
A complete waste of time. You want contams to show, that's why you use agar.
--------------------
You make it all possible. Doesn't it feel good?
|
M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: Doc_T]
#14148363 - 03/19/11 01:18 PM (13 years, 1 month ago) |
|
|
Well, strep and pen won't prevent molds from growing/showing. Molds will grow regardless in the presence of strep and pen. The combination of strep and pen will kill just about any bacterial contamination.
I don't see how contamination "showing" would matter. If you transferred a sector that had some bacteria on it, the bacteria would die (more likely be dead) and the contamination would desist. Mold, on the other hand, would not. Mold is also much easier to see.
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
Edited by M11 (03/19/11 01:35 PM)
|
Fungal growth
Lootinint
Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148382 - 03/19/11 01:23 PM (13 years, 1 month ago) |
|
|
sterile procedure is better and i'm sure cheaper.
|
M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: Fungal growth]
#14148429 - 03/19/11 01:32 PM (13 years, 1 month ago) |
|
|
Cost is nothing in this case. Is mold more prevalent than bacteria as far as contaminations go?
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148450 - 03/19/11 01:37 PM (13 years, 1 month ago) |
|
|
Antibiotic agar is used in cloning wild specimens. And even then it's pretty rare.
--------------------
You make it all possible. Doesn't it feel good?
|
 Trippy_Smurf
Sketchy Mother Fucker
Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage
Last seen: 2 years, 6 months
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148460 - 03/19/11 01:39 PM (13 years, 1 month ago) |
|
|
Penicillin is a mold, so why would you put it somewhere you don't want mold to grow? Mold is like a shot of tequila... once you let one in, it's going to let all it's friends in, and before you know it, your mushrooms will be lying on the floor in a puddle of their own puke.
--------------------
SECURITY: READ THIS!
Chef: Kids, what did I tell you about drugs?
Kids: There's a time and a place for everything, and it's called college.
How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
|
M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: Doc_T]
#14148483 - 03/19/11 01:44 PM (13 years, 1 month ago) |
|
|
Sweet. I think I might just add a bit to the agar for shits. Why not? Is there any reason not to?
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
|
M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: Trippy_Smurf]
#14148490 - 03/19/11 01:45 PM (13 years, 1 month ago) |
|
|
Quote:
Trippy_Smurf said:
Penicillin is a mold, so why would you put it somewhere you don't want mold to grow? Mold is like a shot of tequila... once you let one in, it's going to let all it's friends in, and before you know it, your mushrooms will be lying on the floor in a puddle of their own puke.
What? I am really confused. Mold, tequila, and mushroom puke?
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
|
higgledy-piggledy
Registered: 08/24/10
Posts: 966
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148513 - 03/19/11 01:51 PM (13 years, 1 month ago) |
|
|
Quote:
Trippy_Smurf said:
Penicillin is a mold,
penicillium notatum is a mold, but not penicillin.
Quote:
M11 said:
Why not? Is there any reason not to?
first contact with the substance may sensitize you against it.
if you will have a bacterial infection in the future first the doc will do is give you penicillin.
maybe you are going to develope an allergy against it then.
especially penicillin is known for that.
dont handle stuff you have no frigging idea of.
|
M11
White Thumb
Registered: 06/14/09
Posts: 1,840
|
Re: Adding penicilin G and streptomycin to agar mix? [Re: higgledy-piggledy]
#14148557 - 03/19/11 02:01 PM (13 years, 1 month ago) |
|
|
I have used penicillin G and streptomycin 100s of times for other applications involving agar. I am just curious about the use of penicilin G and streptomycin for cube culturing. I wasn't sure if this technique was used, and it it was, would it be worthwhile.
As far as exposure goes, you don't touch/inhale the stuff, and you use gloves. Besides, you use ridiculously small amounts for agar. Some recipes use about 40 mg of penicilin G and 30 mg of streptomycin (I would have to check my notes, so don't quote me on this) per liter of agar. That is not even close to the dosage for what a doctor would prescribe as medication.
--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas.
-RR
Those mushrooms are fine. Your friend is a pussy.
-RR
Outdoor Patch
|
|
Threaded
Previous
Index
Next
Bulk Cannabis Seeds 
All-In-One Bags That Don't Suck 
Buy Kratom Extract 
North Spore Mushroom Grow Kits & Cultivation Supplies
Edit 
Reply 
DeeBo
Anonymous
Numba9
vivid
keyeghost
1 2 3 all )
arabmobster