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M11
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Registered: 06/14/09
Posts: 1,840
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Adding penicilin G and streptomycin to agar mix?
#14148337 - 03/19/11 01:10 PM (12 years, 10 months ago) |
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Hey folks,
Would it be advisable/worthwhile for me to add penicilin G and streptomycin to my agar mixture? I would add the pen and strep after the agar solution cooled a little bit, but before it would begin to solidify. Pen and strep are temperature sensitive, so they would degrade if they were PCed.
What do you guys think? It would, for the most part, eliminate bacterial contamination. Is mold more of a concern than bacteria as far as contaminations with agar are concerned? Srep and pen aren't going to affect myc any, are they?
M
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148347 - 03/19/11 01:13 PM (12 years, 10 months ago) |
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A complete waste of time. You want contams to show, that's why you use agar.
-------------------- You make it all possible. Doesn't it feel good?
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M11
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Registered: 06/14/09
Posts: 1,840
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Re: Adding penicilin G and streptomycin to agar mix? [Re: Doc_T]
#14148363 - 03/19/11 01:18 PM (12 years, 10 months ago) |
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Well, strep and pen won't prevent molds from growing/showing. Molds will grow regardless in the presence of strep and pen. The combination of strep and pen will kill just about any bacterial contamination.
I don't see how contamination "showing" would matter. If you transferred a sector that had some bacteria on it, the bacteria would die (more likely be dead) and the contamination would desist. Mold, on the other hand, would not. Mold is also much easier to see.
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
Edited by M11 (03/19/11 01:35 PM)
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148382 - 03/19/11 01:23 PM (12 years, 10 months ago) |
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sterile procedure is better and i'm sure cheaper.
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M11
White Thumb

Registered: 06/14/09
Posts: 1,840
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Re: Adding penicilin G and streptomycin to agar mix? [Re: Fungal growth]
#14148429 - 03/19/11 01:32 PM (12 years, 10 months ago) |
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Cost is nothing in this case. Is mold more prevalent than bacteria as far as contaminations go?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148450 - 03/19/11 01:37 PM (12 years, 10 months ago) |
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Antibiotic agar is used in cloning wild specimens. And even then it's pretty rare.
-------------------- You make it all possible. Doesn't it feel good?
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Trippy_Smurf
Sketchy Mother Fucker




Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage
Last seen: 2 years, 4 months
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Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148460 - 03/19/11 01:39 PM (12 years, 10 months ago) |
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Penicillin is a mold, so why would you put it somewhere you don't want mold to grow? Mold is like a shot of tequila... once you let one in, it's going to let all it's friends in, and before you know it, your mushrooms will be lying on the floor in a puddle of their own puke.
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M11
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Registered: 06/14/09
Posts: 1,840
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Re: Adding penicilin G and streptomycin to agar mix? [Re: Doc_T]
#14148483 - 03/19/11 01:44 PM (12 years, 10 months ago) |
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Sweet. I think I might just add a bit to the agar for shits. Why not? Is there any reason not to?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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M11
White Thumb

Registered: 06/14/09
Posts: 1,840
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Re: Adding penicilin G and streptomycin to agar mix? [Re: Trippy_Smurf]
#14148490 - 03/19/11 01:45 PM (12 years, 10 months ago) |
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Quote:
Trippy_Smurf said: Penicillin is a mold, so why would you put it somewhere you don't want mold to grow? Mold is like a shot of tequila... once you let one in, it's going to let all it's friends in, and before you know it, your mushrooms will be lying on the floor in a puddle of their own puke.
What? I am really confused. Mold, tequila, and mushroom puke?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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higgledy-piggledy

Registered: 08/24/10
Posts: 966
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Re: Adding penicilin G and streptomycin to agar mix? [Re: M11]
#14148513 - 03/19/11 01:51 PM (12 years, 10 months ago) |
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Quote:
Trippy_Smurf said: Penicillin is a mold,
penicillium notatum is a mold, but not penicillin.
Quote:
M11 said: Why not? Is there any reason not to?
first contact with the substance may sensitize you against it. if you will have a bacterial infection in the future first the doc will do is give you penicillin. maybe you are going to develope an allergy against it then. especially penicillin is known for that.
dont handle stuff you have no frigging idea of.
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M11
White Thumb

Registered: 06/14/09
Posts: 1,840
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Re: Adding penicilin G and streptomycin to agar mix? [Re: higgledy-piggledy]
#14148557 - 03/19/11 02:01 PM (12 years, 10 months ago) |
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I have used penicillin G and streptomycin 100s of times for other applications involving agar. I am just curious about the use of penicilin G and streptomycin for cube culturing. I wasn't sure if this technique was used, and it it was, would it be worthwhile.
As far as exposure goes, you don't touch/inhale the stuff, and you use gloves. Besides, you use ridiculously small amounts for agar. Some recipes use about 40 mg of penicilin G and 30 mg of streptomycin (I would have to check my notes, so don't quote me on this) per liter of agar. That is not even close to the dosage for what a doctor would prescribe as medication.
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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