|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Matty_Rulez
Lurker, explorer



Registered: 01/13/02
Posts: 269
Loc: Canada
Last seen: 1 month, 16 days
|
Golden Teacher LC to Agar - An experiment..
#14147432 - 03/19/11 08:23 AM (12 years, 10 months ago) |
|
|
I recently innoc'd a few Honey LC's using prints that I made... Golden Teacher and Cambodian strains.. The GT LC showed growth in just over a day, and within a week... The water was crystal clear in just under a week and a half...
Some test jars were made, and have been vigorously colonizing for about 2 weeks now... I had a cpl of extra PDA petrie's in the fridge, so i figured I'd try putting a drop of this GT LC on each petrie, and see what happens...
Anyone ever successfully get an isolate using this method?
A few Pics....
The LC on about day 4 or 5....

Used a small glove box to do the work...

Let's see what happens...

Here's a close up look of one of my test jars... The myc is very thick and fast moving...
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
|
Gonkulator
Ring Modulator


Registered: 09/27/10
Posts: 150
Last seen: 6 years, 6 months
|
Re: Golden Teacher LC to Agar - An experiment.. [Re: Matty_Rulez]
#14147450 - 03/19/11 08:37 AM (12 years, 10 months ago) |
|
|
I did something similar, but I used a MS syringe instead of a print. My agar work at the time failed pretty hard, I'm guessing because it took a bit for my spores to germinate. At some point between germination and the growth of the mycelium, some kind of mold began to cultivate that looked like cubensis mycelium, albeit not the rhizo-growth we try to isolate.
So, I thought this was the beginning of a tedious isolation-transfer-sequence. But in the end, the mold mycelium (cubensis-look-alike) ate any chance that my cubensis spores had. Probably because I didn't catch it when it was just a satellite colony. Granted, using LC-->agar will show results a lot faster, keep in mind (like I learned the hard way), agar work is sort of like micro-managing your project. Check your progress often, and don't let an emulative moldy jerk fool you!
edit: clarity
Edited by Gonkulator (03/19/11 08:38 AM)
|
afrosheen
9Lives the cat



Registered: 03/06/10
Posts: 1,878
Last seen: 2 years, 4 months
|
Re: Golden Teacher LC to Agar - An experiment.. [Re: Gonkulator]
#14147840 - 03/19/11 10:42 AM (12 years, 10 months ago) |
|
|
You have a few different options if you're trying to isolate.
You can let your test jar grow up, then snatch a tiny pin from a cluster and throw it directly onto agar. The rapid growth should outrun any contams. Then you isolate from that dish to other dishes to narrow down the genetics.
You can also put a tiny drop of LC directly onto agar and begin the isolation process from there.
Regardless of which route you take, I'm guessing your goal is to isolate for specific traits, since your LC will be multispore you never know what your first grow from it will look like.
--------------------
|
Matty_Rulez
Lurker, explorer



Registered: 01/13/02
Posts: 269
Loc: Canada
Last seen: 1 month, 16 days
|
Re: Golden Teacher LC to Agar - An experiment.. [Re: afrosheen]
#14148092 - 03/19/11 11:48 AM (12 years, 10 months ago) |
|
|
Cool.... Thanks for the advice... I think I'll try that pin to agar when the time comes... I put one drop of LC per petrie, so I'm expecting to see some growth today or tomorrow..
BTW... Your sig animation ALWAYS makes me laugh!!! .... i was reading one day, and my GF came in the room and burst out laughing cuz it was on the screen... she's like "WTF is that?!?!" 
I'll be updating this post with any changes...
Peace...
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,531 topic views. 20 members, 176 guests and 51 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|