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OfflineBlueLightRain
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Registered: 01/14/11
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Do I Have Trametes versicolor Mycelium?
    #14145883 - 03/18/11 09:56 PM (12 years, 10 months ago)

Hey guys! About a week ago I went out to the mountain and found two different colored forms of Trametes versicolor fruiting from the same fallen log. One was a beatiful orange I hadn't experienced before and the other was a tan and blue variety. I took them home and brought them into the glove box. I peeled the fruits in half and (as carefully as possible) cut out tiny pieces from the inside. I placed a bunch of them on agar and here are some pictures of what is currently growing. Here is a thread, Turkey Tail clone, in which lipa shows some mycelium on agar. Unfortunately I can't tell if mine is similar as it expands. Could you give me your advice on what you think I have?

Here are pictures of the two fruitbodies, just to confirm (yes tiny white pores on underside).


Note: all of these transfers were made on March 11. 7 days ago. Looking for your advice!
A: The "mycelium" is looking slightly thicker than I'm used to seeing from other species. Mold? Any insights?

B: This one is tuft-like and overgrew a bit of bacteria that was surrounding the fragment.

C: A bit wispier. Yes that is an airborne contaminant growing to the right. Gosh-darned glove box.

D: Yes that is also some contaminant on the left. What's odd with this cluster is that the leading edge appears thicker. Mold?

E: This one is baffling. A very thin white mycelial like growth expanded from the fragment practically at the speed of light, covering the dish in about 4 or 5 days. Then yesterday little white puffs began to show. If this isn't mycelium, what mold could this be? This plate was made 7 days ago. I've never seen anything like this.


Thanks for your input!


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Invisiblelipa

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Re: Do I Have Trametes versicolor Mycelium? [Re: BlueLightRain]
    #14151273 - 03/19/11 10:58 PM (12 years, 10 months ago)

Top two look like it. I would transfer some from the top two and see what becomes of it.

Young

And old


Lipa


Edited by lipa (03/20/11 05:44 PM)


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Invisiblemycoelf
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Re: Do I Have Trametes versicolor Mycelium? [Re: lipa]
    #14152902 - 03/20/11 09:29 AM (12 years, 10 months ago)

I would transfer a piece of "B" and then pour an agar sandwich over it to stall the bacteria. TV grows vary similar to reishi. Forming the concentric rings is a tell tale sign. Faster than light colonization is also typical. Growing thick, the forming "shelves" in the petri is also indicative of TV.:goodluck:


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Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

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Invisiblelipa

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Re: Do I Have Trametes versicolor Mycelium? [Re: mycoelf]
    #14153109 - 03/20/11 10:45 AM (12 years, 10 months ago)

No Trametes is not like reishi. Reishi is linear in growth to appresed mat. Trametes is slightly linear to cottony appresed. Just transfer some more of the leading edges of the first and second cultures and see what you come up with.

The rings you speak of are from temperature fluctuations. If you grow the culture at a steady 75F it will be consistent.

Lipa


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InvisibleFractalXplora
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Re: Do I Have Trametes versicolor Mycelium? [Re: lipa]
    #14153572 - 03/20/11 12:29 PM (12 years, 10 months ago)

Glad I seen this post bluelightrain, I just tried the very same thing a month back, unfortunately i only done 5 plates and all exploded in nasty looking stuff, the fruits I used looked a bit water logged and old and they were so rubbery i had problems getting a nice section form the middle of the fruit. I tried the RR 4 jar sterile water bath method with my wedges but the :failboat: came sailing in.

Good luck with your cultures, i hope to try again when i find some better specimens.
IF you get myc, I'd love to trade up some goodies for you. :pm: and keep us posted on your progress. I've been drying them out capping them and giving them to someone who needs 'em.

The tea is great too, gotta love the turkey tail.:thumbup:

heres my patch;as you can see it was pretty cold and frosty when I picked them. Maybe not the best of times, I dont really know.






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Edited by FractalXplora (03/20/11 12:36 PM)


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OfflineBlueLightRain
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Re: Do I Have Trametes versicolor Mycelium? [Re: FractalXplora]
    #14154326 - 03/20/11 02:53 PM (12 years, 10 months ago)

Quote:


FractalXplora said:
Good luck with your cultures, i hope to try again when i find some better specimens.
IF you get myc, I'd love to trade up some goodies for you. :pm: and keep us posted on your progress. I've been drying them out capping them and giving them to someone who needs 'em.

The tea is great too, gotta love the turkey tail.:thumbup:





Thanks FractalXplora. I don't want to the jump the gun and I hate making promises in the event things fail, but if I do have a successful culture then wooooohoooooo baby I'm trading that one! Heehee. I'll keep you guys posted on my transfers and updates. I'll be doing some transfers in the next half and hour, give it a week or so to see some good results. If it's growing at this rate  in coastal California winter weather it should be a happy fungus :smile:.

A few days ago I boiled the rest of what I found and my goodness it's damn good. None of the bitterness of Reishi. Earthy. Can definitely be drank without honey. I've been infusing it with local Salvia leucophyllum and Salvia alpiana, and some local rosemary picked off people's front yards. It's great :smile:.


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Edited by BlueLightRain (03/20/11 04:03 PM)


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