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freeskierpj
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BRF Agar Substitute without a PC?
#14145167 - 03/18/11 07:50 PM (12 years, 10 months ago) |
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Can it be done? If so how long would you steam sterilize? I would imagine since PF tek Jars can be steam sterilized that a BRF agar substitute could be as well but I haven't found anything on the subject with all my searching over the past few days...
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    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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Doc_T
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145182 - 03/18/11 07:53 PM (12 years, 10 months ago) |
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Like a jar with half an inch of brf/verm to do tissue clones on? Steam like an hour or 90 mins or whatever. 45 would probably work. But go get a pc for ten bucks at Goodwill.
-------------------- You make it all possible. Doesn't it feel good?
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: Doc_T]
#14145208 - 03/18/11 07:58 PM (12 years, 10 months ago) |
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Basically...just following this tek but it only covers using a PC. And yea I know but right now it's tough times just being able to pay the rent as I just moved and am still monetarily recovering from first last and security...
Here's the tek: https://www.shroomery.org/8514/Agar-substitute
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. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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jokefox
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145229 - 03/18/11 08:01 PM (12 years, 10 months ago) |
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basically they tek is using anything that mycelium will grow on
you can use cardboard, wbs, BRF, anything in a thin layer
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faceyneck
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145372 - 03/18/11 08:25 PM (12 years, 10 months ago) |
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Quote:
freeskierpj said: Basically...just following this tek but it only covers using a PC. And yea I know but right now it's tough times just being able to pay the rent as I just moved and am still monetarily recovering from first last and security...
Here's the tek: https://www.shroomery.org/8514/Agar-substitute
I was going to do this as an experiment myself.
I see absolutely no reason why it wouldn't work. In fact, it would probably MORE likely become sterilized than PF cakes done with the same sterilization process.
Were you just curious, PJ? You've been using grain, so I assumed you had a PC.
-------------------- Anything posted here, is total bullshit. My Meyers-Briggs Personality: INTJ New growers, or anyone else just needing help; I'm always glad to help right here.
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: faceyneck]
#14145477 - 03/18/11 08:46 PM (12 years, 10 months ago) |
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I've only used grain once and that's when I bought 3 2lb bags of WBS through mycopath pre-sterilized. Other than that my latest is BRF to coir to straw for the mono I'm doing now...I'm trying it out right now. Gonna do 45 minutes and see how it goes. I got this thing at AC Moore that is like stacked petri dishes that screw on so I'm also trying it with that. Will post back results tomorrow as I'm not gonna be up late enough to inoculate it tonight...
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. -ProfessorPinHead
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crp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145556 - 03/18/11 09:03 PM (12 years, 10 months ago) |
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I just wanna follow this thread 
...o yeah, sweet clone by the way.
-------------------- "The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man." My Journal Entry
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: crp32008]
#14145705 - 03/18/11 09:29 PM (12 years, 10 months ago) |
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Haha thanks CRP, decided she's gonna end up being a mommy and I'll take clones from her
--------------------
. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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crp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145729 - 03/18/11 09:33 PM (12 years, 10 months ago) |
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Always a good solution! I never could keep a mother in veg for long...bitch always wanted to flower on me...couldn't say I was ever too disappointed with that
-------------------- "The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man." My Journal Entry
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145753 - 03/18/11 09:36 PM (12 years, 10 months ago) |
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Even on 24/0?
--------------------
. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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crp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145785 - 03/18/11 09:40 PM (12 years, 10 months ago) |
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yup yup, back in school I had a B52 mother and K2 father (father was used for seeding the ones I wanted for breeding). Both of them under 24/0 and started flowering after 8 or 9 mos or being parents. Sortof a shame hahaha I havent grown in so long I dont even know if those strains are any good anymore...breeders are so quick now a days with new strains every week it seems lol
-------------------- "The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man." My Journal Entry
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145904 - 03/18/11 09:59 PM (12 years, 10 months ago) |
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Did you prune and take cuttings? That's strange cause I don't see why they would just randomly flower hahaha
--------------------
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crp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14145959 - 03/18/11 10:08 PM (12 years, 10 months ago) |
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It must have been my technique or lack of lol I would have a ton of plants but I wasn't an expert. Just did mediocre with it. I didnt prune well, I took cuttings and once I saw them flowering I shaved off the bottom 1/3-1/2. I don't have a large amount of botany skills but I can do ok. I did notice a lot of mine would flower automatically after 6-7, 8 months of vegging.
-------------------- "The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man." My Journal Entry
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: crp32008]
#14150931 - 03/19/11 09:46 PM (12 years, 10 months ago) |
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So after 3 failures;
1) Regular mouth 1/2 pints not the wide mouth plus 90 minutes just made it practically a solid (tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not this way but I would shourten the time for any future experiments.
2) Regular mouth 1/2 pints not the wide mouth plus 60 minutes just made it practically a solid same as 90 minutes (again tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not but I'm gonna shorten the time again...
3) Plastc bead holders (as I had no empty jars and was way high so I figured what the heck, why not. They are stackable and twist into place and had 5 compartments...they weren't PP5 I guess haha even at 45 minutes they were waaaaaaaay bent and shit.
I sat and thought about it and remembered the Pill Divider Agar Tek I used 1cc of water and .3g of BRF per "day", sterilized for 45 minutes again and what do ya know nothing seemed to go wrong! I opened only 1 section up to check consistency and it sounded exactly like that of the BRF Agar Substitute made with a pressure cooker...I let it cool overnight and then inoculated Sunday with ONE DROP of Dancing Tiger MS that had germinated in the mail on the way here, Monday with ONE DROP of x7x that was supposed to be LC but it just looked more like MS solution to me, Tuesday with ONE DROP of GT (practically my last drop so I'm hoping for it to work out), and Wednesday with a tiny pin from my African Transkei BRF jar that FINALLY (7 weeks) colonized fully).
Unfortunately the BRF turns white during sterilization and I can't tell if anything is working out...gonna do another batch with a drop of food coloring (or pigment of some sort, any suggestions?) so that I can actually see the mycelium if this does work.
If so this would be a very easy intro to agar for newbs or for those that have no pressure cooker like myself.
--------------------
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14151142 - 03/19/11 10:29 PM (12 years, 10 months ago) |
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Cool. I thought I was out of business when my PC blew. Now, I can save that FALBINO even if it did contam. Sweet!
Pill boxt is sweet idea, too. Almost chucked one the other day but thought..."this will be good for something"*. Glad I saved it now!
*Reminds me of George Carlin."Well smell it. Its still good! Someone is saving that. It'll turn up in something..."
mmmmm Meat-Cake
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14151223 - 03/19/11 10:48 PM (12 years, 10 months ago) |
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i use food coloring in my dishes, <3 the purple haha

works like a charm, except i add it before i sterilize it
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14151607 - 03/19/11 11:55 PM (12 years, 10 months ago) |
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I kinda figured that. How I filled these is BRF then water then shake so I'd just add it to the water before adding that to the BRF.
--------------------
. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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crp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14151683 - 03/20/11 12:11 AM (12 years, 10 months ago) |
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I believe he probably mixes all the dry ingredients first, then add water, then if you were to food color add the color? Adding BRF is just like adding malt or dextrose to agar. I would assume with brf you would have to add a good amount less than you would for dextrose and others.
-------------------- "The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man." My Journal Entry
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jokefox
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14153176 - 03/20/11 11:03 AM (12 years, 10 months ago) |
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Quote:
biologys said: i use food coloring in my dishes, <3 the purple haha

works like a charm, except i add it before i sterilize it
i really like how your purple turned out i usually use red or green
do you notice any color fading after some time i can only hold color on my plate for a week or two
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biologys
Mycologist in Trainning




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Re: BRF Agar Substitute without a PC? [Re: jokefox]
#14153540 - 03/20/11 12:22 PM (12 years, 10 months ago) |
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nope, still just as purple as it was when i put them on plates, and some are a month old now
i get that color purple by using green agar stick's with red and yellow food coloring.
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14154090 - 03/20/11 02:08 PM (12 years, 10 months ago) |
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I have noticed color fade in LCs. One of my favorite LC is diluted apple cider. (Dont laugh! Its AWESOME) It starts out brown, but the myc takes on the brown color and the liquid becomes clear after a week or so.
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154176 - 03/20/11 02:23 PM (12 years, 10 months ago) |
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yep LC's start out a golden color, and as the mycelium consumes the sugar's ( honey, or LME ) it turns clearer.
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14154308 - 03/20/11 02:47 PM (12 years, 10 months ago) |
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I know this is WAY off topic-sorry-but just curious. Does the myc expel waste products as it digests the sugars?
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154333 - 03/20/11 02:54 PM (12 years, 10 months ago) |
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from my understanding it secrets the myc piss to break things down so it can digest it?
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14154344 - 03/20/11 02:56 PM (12 years, 10 months ago) |
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The yellow stuff that pools at the bottom of jars sometimes?
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154353 - 03/20/11 02:57 PM (12 years, 10 months ago) |
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yes.
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3Cajun1Mo8
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154356 - 03/20/11 02:58 PM (12 years, 10 months ago) |
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I have tried this with success. I only Steamed for 30 minutes since it wa just a 1/2 inch of BRF in an old olive jar .
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: 3Cajun1Mo8]
#14154373 - 03/20/11 03:03 PM (12 years, 10 months ago) |
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Is it the same ratio as the PF Tek? 2:1:1?
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freeskierpj
Just trying to learn some shit



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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154413 - 03/20/11 03:09 PM (12 years, 10 months ago) |
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EDIT: Damn bio you beat me to it! And for the love of god don't call it myc piss!!! Every post I've seen where RR comments on the whole "myc piss=metabolites" he get's upset No but seriously just say metabolites it's not that hard 
Just a very quick search but found THIS:
Quote:
Metabolites are also the natural product of the mycelia's colonization. The outside surface of the mycelia exudes these chemicals to break down and digest the nutrients/minerals that are made available through this process. Most mycelia can actually mineralize rock as it secretes oxalic acid.
I'm interested in this as well and although it sounds like he knows what he's talking about I never like to trust just a single source so I'm gonna keep doing some research...I need to get some food coloring though are there any other alternatives to food coloring that may work for this?
Also I might try this but instead of just adding water I would add a solution of water and:
Just to see if the added nutrients would help speed the process up. This is of course once I get my food coloring and make sure that it does actually work and that the myc eats up the BRF agar inside of these.
Oh and one last question, they aren't 100% fully sealed (poor construction of the lids but hey for 99 cents what can you ask for) so would that be a good enough source of GE/FAE or whatever agar needs in order for the spores to germinate?
--------------------
. -ProfessorPinHead
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154456 - 03/20/11 03:18 PM (12 years, 10 months ago) |
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I'd bet coffee wouldn't be bad, either.
So, is this stuff stiff, or really wet, or gluey?
RR def seems to know his stuff. I just saw this quote from him that made me chuckle:
"Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas." -RR
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14154486 - 03/20/11 03:25 PM (12 years, 10 months ago) |
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Quote:
3Cajun1Mo8 said: I have tried this with success. I only Steamed for 30 minutes since it wa just a 1/2 inch of BRF in an old olive jar .

Interesting. Did you use the same tek I was using HERE, and just scale it up? Just as an easy example, 5g brf to 16cc water means 10g of brf to 32cc of water. If not can you give some more insight on your technique?
Quote:
bateyes88 said: I'd bet coffee wouldn't be bad, either.
So, is this stuff stiff, or really wet, or gluey?
RR def seems to know his stuff. I just saw this quote from him that made me chuckle:
"Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas." -RR
Good point, this is definitely gonna be something I'll mess around with for a while. And it's more like a gooey slop...I can't really describe the texture too well first off and second I used self ground BRF so it was a little more chunky than if you were to buy BRF from a supermarket...
--------------------
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14154519 - 03/20/11 03:32 PM (12 years, 10 months ago) |
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Cool thanks. Thats all I needed. Just wondering if my "glop" was what it is supposed to be lol. I grind my own, too. Burnt out a couple grinders, but its worth it. Cant beat $.30/lb. And fresh works better, too. Where I live I bet they dont sell but a bag or two a year.
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3Cajun1Mo8
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14154548 - 03/20/11 03:37 PM (12 years, 10 months ago) |
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Yeah thats the tek I used, just instead of a 1/2 pint jar I used a Olive jar I had just finished and cleaned with a polyfillid screwed on over tyvek.
I ended up only using the BRF mixture once before i chunked it out, I took a piece out and put it in some Honey water I prepped. The little chunk of colonized BRF just floated around and mycelium webbed out across the top of the honey water . then nocced up a grain jar with the floating web and that grain jar is doing fantastic.
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14155192 - 03/20/11 06:08 PM (12 years, 10 months ago) |
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Quote:
bateyes88 said: Cool thanks. Thats all I needed. Just wondering if my "glop" was what it is supposed to be lol. I grind my own, too. Burnt out a couple grinders, but its worth it. Cant beat $.30/lb. And fresh works better, too. Where I live I bet they dont sell but a bag or two a year.
Yea glop is a perfect word to describe it hahaha and seems like mine is working! Can't see it well but there is a thin film of mycelium covering the pin here.
 And yes PJ is in his PJs...they're comfy as hell!
--------------------
. -ProfessorPinHead
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14155346 - 03/20/11 06:38 PM (12 years, 10 months ago) |
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So, for half pint reg mouth (normal PF Tek jar) 90 minutes is what you came up w/? Is it still "Gloppy" after the 90 min, or does it firm up?
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14155363 - 03/20/11 06:42 PM (12 years, 10 months ago) |
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wait, so you're just trying to get an agar substitute that you can use with out a PC, or does it have to be BRF?
or are you using BRF and water, with no agar lol im a bit confused.
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14155451 - 03/20/11 06:57 PM (12 years, 10 months ago) |
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I think an agar sub for use when a PC is unavailable. At least thats what I need, and I think the OP as well...
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14155905 - 03/20/11 08:21 PM (12 years, 10 months ago) |
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Yea I'm following this tek HERE but just added the little twist of doing it in a pill divider after having seen the Pill Divider Agar Tek and for the reason that I have no wide mouthed half pint jars, only regular mouth (not tapered or anything so it still works fine for the PF tek) but it would be a royal PITA to get a little wedge out of there as they are taller and skinnier. I tried using a few other types of containers and still have a few to try out but I wanna be able to tell if my LC's are clean and this seems like the easiest way to do it. Right now I have 3 empty pill containers and the one that I used as a test so that would mean I could check 28 different LC's for their purity and then use those LC's on pasteurized poo/straw/verm/coir (or some variation of that) bags like I was asking about with the hpoo and gypsum question.
I tried doing a pill divider LC tek a few weeks back but it didn't work as the pill dividers I have for some reason aren't 100% air tight so with the BRF agar I covered it in micropore tape around any areas that could let contams in.
Bio - if you have a different agar substitute that doesn't require a PC please fill me in 
Bateyes - As I stated HERE right after number 3:
Quote:
freeskierpj said: So after 3 failures;
1) Regular mouth 1/2 pints not the wide mouth plus 90 minutes just made it practically a solid (tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not this way but I would shourten the time for any future experiments.
2) Regular mouth 1/2 pints not the wide mouth plus 60 minutes just made it practically a solid same as 90 minutes (again tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not but I'm gonna shorten the time again...
3) Plastc bead holders (as I had no empty jars and was way high so I figured what the heck, why not. They are stackable and twist into place and had 5 compartments...they weren't PP5 I guess haha even at 45 minutes they were waaaaaaaay bent and shit.
I sat and thought about it and remembered the Pill Divider Agar Tek I used 1cc of water and .3g of BRF per "day", sterilized for 45 minutes again and what do ya know nothing seemed to go wrong! I opened only 1 section up to check consistency and it sounded exactly like that of the BRF Agar Substitute made with a pressure cooker...I let it cool overnight and then inoculated Sunday with ONE DROP of Dancing Tiger MS that had germinated in the mail on the way here, Monday with ONE DROP of x7x that was supposed to be LC but it just looked more like MS solution to me, Tuesday with ONE DROP of GT (practically my last drop so I'm hoping for it to work out), and Wednesday with a tiny pin from my African Transkei BRF jar that FINALLY (7 weeks) colonized fully).
Unfortunately the BRF turns white during sterilization and I can't tell if anything is working out...gonna do another batch with a drop of food coloring (or pigment of some sort, any suggestions?) so that I can actually see the mycelium if this does work.
If so this would be a very easy intro to agar for newbs or for those that have no pressure cooker like myself.
So no 90 minutes caused mine to go solid so I would just mess around with it and try different times but 45 minutes worked for mine.
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    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14155996 - 03/20/11 08:35 PM (12 years, 10 months ago) |
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Right. I wasnt sure if solid was a good thing or not, lol. Ive been reading old posts about it and seems like "rubbery" is what to shoot for.
I know you said "glop", but I thought you meant before steaming so I was unsure what the final product looks like.
One issue that kept coming up is that w/ BRF "agar" is that while it looks OK, there may be contams that wont show up on BRF agar, but will when spawned to grain. Could just be the normal "shroomery poo-pooers" that crap on ideas that are not thier own. I'm still gonna try it. Different strokes 4 different folks!
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14156457 - 03/20/11 09:43 PM (12 years, 10 months ago) |
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so you are actually using AGAR just not pc'n it..
all you need is a nutritional item, so just boil some potatoes, and use the water in your agar mix, or sugar in the raw...etc...and steam it for 60 minutes or 90 mins they say grains has to be PC sterilized, but sugar and liquid additives dont..so i'd say sugar, or honey agar should work being steamed..idk
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14156482 - 03/20/11 09:48 PM (12 years, 10 months ago) |
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I think op is using BRF instead of agar. Just BRF and water, or BRF, Verm, and water. No agar. Just thick rice flour mixture in a thin layer.
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biologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14156526 - 03/20/11 09:55 PM (12 years, 10 months ago) |
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see, that was why i asked alil while ago, cause i was confused...why not use agar is super cheap and easier then brf paste to sterilize lol
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14156530 - 03/20/11 09:56 PM (12 years, 10 months ago) |
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No sorry if it wasn't clear but I am just using BRF and water like I said
Quote:
freeskierpj said: Yea I'm following this tek HERE
--------------------
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14156546 - 03/20/11 09:58 PM (12 years, 10 months ago) |
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Quote:
biologys said: see, that was why i asked alil while ago, cause i was confused...why not use agar is super cheap and easier then brf paste to sterilize lol
Without a pressure cooker?
--------------------
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    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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biologys
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14156573 - 03/20/11 10:01 PM (12 years, 10 months ago) |
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I dont see why not, its basicly a liquid, so as long as you get the temp up and keep them for 60-90 minutes it should sterilize it..
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14156626 - 03/20/11 10:08 PM (12 years, 10 months ago) |
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I just tried just that. I cleaned a pyrex measuring alcohol w/ IPA, and microwatved the water, added premade LMA powder, and nuked to boiling again. Then I poured into sterile petris opening just enough to quickly pour some in. I figure if they are still clean in a few days then Ill be fine. We'll see! Hopefully it works as Its very hard to find agar here and expensive online. Wish I could find a good used PC cheap!
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14156734 - 03/20/11 10:24 PM (12 years, 10 months ago) |
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I hope it does work as I'd love to do some agar work!
--------------------
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14156826 - 03/20/11 10:35 PM (12 years, 10 months ago) |
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I am guessing it will work, but I bet Ill have a much higher initial contam rate
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14156833 - 03/20/11 10:37 PM (12 years, 10 months ago) |
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That was weird. Ive never been on during the 4 am back-up before!
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freeskierpj
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14157005 - 03/20/11 11:07 PM (12 years, 10 months ago) |
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Lol oh I hate the 4 am back up...mine comes at 6am and when I end up on an adderall binge that's my "Oh shit I spent alllllllllll night on the shroomery" wake up call hahahaha...
--------------------
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    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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Doc_T
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14158162 - 03/21/11 07:00 AM (12 years, 10 months ago) |
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Quote:
bateyes88 said: Its very hard to find agar here. Wish I could find a good used PC cheap!
Where are you?
Also, thrift stores have PCs.
-------------------- You make it all possible. Doesn't it feel good?
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ProfessorPinHead
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
#14158180 - 03/21/11 07:13 AM (12 years, 10 months ago) |
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Quote:
freeskierpj said: Lol oh I hate the 4 am back up...mine comes at 6am and when I end up on an adderall binge that's my "Oh shit I spent alllllllllll night on the shroomery" wake up call hahahaha...
expand this thumb nail and read me 
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biologys
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i love the back up, i play the game, and always get a high score, but it never saves it
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14159285 - 03/21/11 01:24 PM (12 years, 10 months ago) |
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Now I feel MUCH better, PP. Thanks!lol
Im in East Bumfuct midcoast Maine
Closest agar is Portland 100 miles away. Checked oriental section of "mega-mart" supermarkets. There is like 10 non-whites in the whole state. Even Brown Rice has an inch layer of dust on it.
I should buy a pant-load when I do make it to the city, but it never happens for various reasons. I used the last of my LMA powder last night making 6 dishes w/o PCing em. See how that goes...
Do I need to parafilm the dishes before I nocc em up? Your supposted to keep em upside down for condensatsion, right? Seems like contams can get in the rim if upsidedown and not filmed...
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Doc_T
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
#14159300 - 03/21/11 01:27 PM (12 years, 10 months ago) |
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Quote:
bateyes88 said: "mega-mart" supermarkets.
You don't have a health food store? Mountain Man's Healthy Co-Op or something?
-------------------- You make it all possible. Doesn't it feel good?
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bateyes88
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Re: BRF Agar Substitute without a PC? [Re: Doc_T]
#14159430 - 03/21/11 01:57 PM (12 years, 10 months ago) |
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There is a members-only one- its very small...I think the public can go, just have to pay extra or somrthing. Not too sure.
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faceyneck
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Re: BRF Agar Substitute without a PC? [Re: biologys]
#14162426 - 03/22/11 12:00 AM (12 years, 10 months ago) |
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Quote:
biologys said: i love the back up, i play the game, and always get a high score, but it never saves it 
how do you know you got a high score? 
I made it to level 7 once. I think that's my highest.
-------------------- Anything posted here, is total bullshit. My Meyers-Briggs Personality: INTJ New growers, or anyone else just needing help; I'm always glad to help right here.
We give cultivation advice here. AMU Q & A - We're glad to help My Doggy Door Greenhouse! First Ever Shmuvbox Tek! Do Manure Right!
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biologys
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Re: BRF Agar Substitute without a PC? [Re: faceyneck]
#14162442 - 03/22/11 12:04 AM (12 years, 10 months ago) |
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well, high compared to what i usd to get when i first started lol i can get massive curve ball bonus's haha
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