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Offlinefreeskierpj
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BRF Agar Substitute without a PC?
    #14145167 - 03/18/11 07:50 PM (12 years, 11 months ago)

Can it be done? If so how long would you steam sterilize? I would imagine since PF tek Jars can be steam sterilized that a BRF agar substitute could be as well but I haven't found anything on the subject with all my searching over the past few days...


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InvisibleDoc_T
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145182 - 03/18/11 07:53 PM (12 years, 11 months ago)

Like a jar with half an inch of brf/verm to do tissue clones on?
Steam like an hour or 90 mins or whatever. 45 would probably work.
But go get a pc for ten bucks at Goodwill.


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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: Doc_T]
    #14145208 - 03/18/11 07:58 PM (12 years, 11 months ago)

Basically...just following this tek but it only covers using a PC. And yea I know but right now it's tough times just being able to pay the rent as I just moved and am still monetarily recovering from first last and security...

Here's the tek:
https://www.shroomery.org/8514/Agar-substitute


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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145229 - 03/18/11 08:01 PM (12 years, 11 months ago)

basically they tek is using anything that mycelium will grow on

you can use cardboard, wbs, BRF, anything in a thin layer

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Offlinefaceyneck
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145372 - 03/18/11 08:25 PM (12 years, 11 months ago)

Quote:

freeskierpj said:
Basically...just following this tek but it only covers using a PC. And yea I know but right now it's tough times just being able to pay the rent as I just moved and am still monetarily recovering from first last and security...

Here's the tek:
https://www.shroomery.org/8514/Agar-substitute




I was going to do this as an experiment myself.

I see absolutely no reason why it wouldn't work. In fact, it would probably MORE likely become sterilized than PF cakes done with the same sterilization process.

Were you just curious, PJ? You've been using grain, so I assumed you had a PC. :justdontknow:


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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: faceyneck]
    #14145477 - 03/18/11 08:46 PM (12 years, 11 months ago)

I've only used grain once and that's when I bought 3 2lb bags of WBS through mycopath pre-sterilized. Other than that my latest is BRF to coir to straw for the mono I'm doing now...I'm trying it out right now. Gonna do 45 minutes and see how it goes. I got this thing at AC Moore that is like stacked petri dishes that screw on so I'm also trying it with that. Will post back results tomorrow as I'm not gonna be up late enough to inoculate it tonight...


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Offlinecrp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145556 - 03/18/11 09:03 PM (12 years, 11 months ago)

:trolldance:  I just wanna follow this thread  :trolldance:

...o yeah, sweet clone by the way.


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"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: crp32008]
    #14145705 - 03/18/11 09:29 PM (12 years, 11 months ago)

Haha thanks CRP, decided she's gonna end up being a mommy and I'll take clones from her :smile:


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Offlinecrp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145729 - 03/18/11 09:33 PM (12 years, 11 months ago)

Always a good solution!  I never could keep a mother in veg for long...bitch always wanted to flower on me...couldn't say I was ever too disappointed with that :laugh2:


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"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145753 - 03/18/11 09:36 PM (12 years, 11 months ago)

Even on 24/0?


--------------------

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Offlinecrp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145785 - 03/18/11 09:40 PM (12 years, 11 months ago)

yup yup, back in school I had a B52 mother and K2 father (father was used for seeding the ones I wanted for breeding).  Both of them under 24/0 and started flowering after 8 or 9 mos or being parents.  Sortof a shame hahaha  I havent grown in so long I dont even know if those strains are any good anymore...breeders are so quick now a days with new strains every week it seems lol


--------------------
"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

My Journal Entry

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145904 - 03/18/11 09:59 PM (12 years, 11 months ago)

Did you prune and take cuttings? That's strange cause I don't see why they would just randomly flower hahaha :smile:


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Offlinecrp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14145959 - 03/18/11 10:08 PM (12 years, 11 months ago)

It must have been my technique or lack of lol  I would have a ton of plants but I wasn't an expert.  Just did mediocre with it. I didnt prune well, I took cuttings and once I saw them flowering I shaved off the bottom 1/3-1/2.  I don't have a large amount of botany skills but I can do ok.  I did notice a lot of mine would flower automatically after 6-7, 8 months of vegging.


--------------------
"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

My Journal Entry

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: crp32008]
    #14150931 - 03/19/11 09:46 PM (12 years, 11 months ago)

So after 3 failures;

1) Regular mouth 1/2 pints not the wide mouth plus 90 minutes just made it practically a solid (tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not this way but I would shourten the time for any future experiments.

2) Regular mouth 1/2 pints not the wide mouth plus 60 minutes just made it practically a solid same as 90 minutes (again tried colonizing with an LC syringe I knew was clean so I guess we'll see if it actually works or not but I'm gonna shorten the time again...

3) Plastc bead holders (as I had no empty jars and was way high so I figured what the heck, why not. They are stackable and twist into place and had 5 compartments...they weren't PP5 I guess haha even at 45 minutes they were waaaaaaaay bent and shit.

I sat and thought about it and remembered the Pill Divider Agar Tek I used 1cc of water and .3g of BRF per "day", sterilized for 45 minutes again and what do ya know nothing seemed to go wrong! I opened only 1 section up to check consistency and it sounded exactly like that of the BRF Agar Substitute made with a pressure cooker...I let it cool overnight and then inoculated Sunday with ONE DROP of Dancing Tiger MS that had germinated in the mail on the way here, Monday with ONE DROP of x7x that was supposed to be LC but it just looked more like MS solution to me, Tuesday with ONE DROP of GT (practically my last drop so I'm hoping for it to work out), and Wednesday with a tiny pin from my African Transkei BRF jar that FINALLY (7 weeks) colonized fully).

Unfortunately the BRF turns white during sterilization and I can't tell if anything is working out...gonna do another batch with a drop of food coloring (or pigment of some sort, any suggestions?) so that I can actually see the mycelium if this does work.

If so this would be a very easy intro to agar for newbs or for those that have no pressure cooker like myself.


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Offlinebateyes88
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14151142 - 03/19/11 10:29 PM (12 years, 11 months ago)

Cool. I thought I was out of business when my PC blew. Now, I can save that FALBINO even if it did contam. Sweet!

Pill boxt is sweet idea, too. Almost chucked one the other day but thought..."this will be good for something"*. Glad I saved it now!

*Reminds me of George Carlin."Well smell it. Its still good! Someone is saving that. It'll turn up in something..."

mmmmm Meat-Cake

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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: bateyes88]
    #14151223 - 03/19/11 10:48 PM (12 years, 11 months ago)

i use food coloring in my dishes, <3 the purple haha



works like a charm, except i add it before i sterilize it

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Offlinefreeskierpj
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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14151607 - 03/19/11 11:55 PM (12 years, 11 months ago)

I kinda figured that. How I filled these is BRF then water then shake so I'd just add it to the water before adding that to the BRF.


--------------------

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Offlinecrp32008
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Re: BRF Agar Substitute without a PC? [Re: freeskierpj]
    #14151683 - 03/20/11 12:11 AM (12 years, 11 months ago)

I believe he probably mixes all the dry ingredients first, then add water, then if you were to food color add the color?  Adding BRF is just like adding malt or dextrose to agar.  I would assume with brf you would have to add a good amount less than you would for dextrose and others.


--------------------
"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

My Journal Entry

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Re: BRF Agar Substitute without a PC? [Re: biologys]
    #14153176 - 03/20/11 11:03 AM (12 years, 11 months ago)

Quote:

biologys said:
i use food coloring in my dishes, <3 the purple haha



works like a charm, except i add it before i sterilize it




i really like how your purple turned out
i usually use red or green

do you notice any color fading after some time
i can only hold color on my plate for a week or two

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Invisiblebiologys
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Re: BRF Agar Substitute without a PC? [Re: jokefox]
    #14153540 - 03/20/11 12:22 PM (12 years, 11 months ago)

nope, still just as purple as it was when i put them on plates, and some are a month old now


i get that color purple by using green agar stick's with red and yellow food coloring.

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