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OfflineOoBYCoO
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OoBYCoO's Homemade Inoculation Loop TEK! * 3
    #14144648 - 03/18/11 06:20 PM (13 years, 13 days ago)

OoBYCoO's Homemade Inoculation Loop TEK!!!
Shout-out to eLShaMukO for giving me the idea!!!

First you need:
  • 4 twisty ties, I used the ones you get w/ a loaf of bread.
  • Tape
  • Lighter
  • Handle- I used a wooden stick that normally goes into those fragrant oil bottles and act as a wick.



Now pair up the ties and twist them together like so...


Now take your lighter and burn off the paper/plastic...


...and you should end up w/ this...


Now twist those two together like so...


Now, do this...


Fasten it to your handle


Tape


DONE!!!  :super:

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Invisiblejokefox
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #14144754 - 03/18/11 06:37 PM (13 years, 13 days ago)

why not just get some thick gauge wire,

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OfflineMushroom_J
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: jokefox]
    #14144808 - 03/18/11 06:46 PM (13 years, 13 days ago)

High e guitar string could work since the tend to break easy :P


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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: jokefox]
    #14144875 - 03/18/11 06:59 PM (13 years, 13 days ago)

Quote:

jokefox said:
why not just get some thick gauge wire,



I guess you could but...  w/ this way they get red hot super fast.  A thicker gauge wire would take longer to flame sterilize IMO.  :shrug:

Edited by OoBYCoO (03/18/11 06:59 PM)

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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: Mushroom_J]
    #14144886 - 03/18/11 07:00 PM (13 years, 13 days ago)

Quote:

Mushroom_J said:
High e guitar string could work since the tend to break easy :P



I have no experience w/ them so I couldn't say.

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Invisiblejokefox
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #14144958 - 03/18/11 07:13 PM (13 years, 13 days ago)

Quote:

OoBYCoO said:
Quote:

jokefox said:
why not just get some thick gauge wire,



I guess you could but...  w/ this way they get red hot super fast.  A thicker gauge wire would take longer to flame sterilize IMO.  :shrug:




good call

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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: jokefox]
    #14144980 - 03/18/11 07:16 PM (13 years, 13 days ago)

:super:

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InvisibleDZ
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #14145076 - 03/18/11 07:35 PM (13 years, 13 days ago)

cool idea and nice write-up. but i would not do any sterile work with tape that close to my work. other than that, it looks pretty good.

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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: DZ]
    #14145433 - 03/18/11 08:36 PM (13 years, 13 days ago)

I do plenty of sterile work and it NEVER has been an issue.

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Offlinecrp32008
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #14145521 - 03/18/11 08:55 PM (13 years, 13 days ago)

This is a bangin' little tek ya got here!!! I just had to replace my scalpel and it had a round top so I used to use it as an inoc loop as well but, my new one isnt as cool so I made one of these bad boys...what great innovation dude :muffin:


--------------------
"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: crp32008]
    #14145529 - 03/18/11 08:56 PM (13 years, 13 days ago)

:super:

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Offline00Dan
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #16209826 - 05/10/12 12:45 PM (11 years, 10 months ago)

Sorry for bumping an old thread, but this one is the newest for homemade loops-

For a handle, consider an old style x-acto knife handle.  Just put your twist tie in where the blade fits.  $3 at any office supply store.

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OfflinePocketRevolution
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: 00Dan]
    #16211598 - 05/10/12 07:12 PM (11 years, 10 months ago)

It seems it's almost as simple as affixing *some type of wire* to *some type of handle*.  Maybe an inoculation loop is nothing more than a small loop of wire with a handle?

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Offline00Dan
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: PocketRevolution]
    #16214049 - 05/11/12 07:33 AM (11 years, 10 months ago)

True..... but I see alot of people using wood while the x-acto handle can be PC'd to sterilize it.

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OfflineOoBYCoO
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: 00Dan]
    #16225272 - 05/13/12 04:10 PM (11 years, 10 months ago)

Yeah, you could do that no problem!

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Offlineforestly
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: OoBYCoO]
    #18677033 - 08/08/13 09:04 AM (10 years, 7 months ago)

Cool tek! The only so-so problem i see with it is that when you twist them together there are many spaces where contamination can hide. So sterilizing with alcohol would not be perfect. But if you sterilize this DIY loop in a PC then it would work as a charm.

Thanks man, i was just ordering some inoc loops but your TEK gave me some ideas for DIY.

:bonghit::takingnotes:


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OfflinePsilicon
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: forestly]
    #18677060 - 08/08/13 09:14 AM (10 years, 7 months ago)

Quote:

forestly said:
Cool tek! The only so-so problem i see with it is that when you twist them together there are many spaces where contamination can hide. So sterilizing with alcohol would not be perfect. But if you sterilize this DIY loop in a PC then it would work as a charm.
:bonghit::takingnotes:




That's why you heat it to red hot before you use it.

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InvisibleDoc_D
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: forestly]
    #18677254 - 08/08/13 10:13 AM (10 years, 7 months ago)

Quote:

forestly said:
Cool tek! The only so-so problem i see with it is that when you twist them together there are many spaces where contamination can hide. So sterilizing with alcohol would not be perfect. But if you sterilize this DIY loop in a PC then it would work as a charm.

Thanks man, i was just ordering some inoc loops but your TEK gave me some ideas for DIY.

:bonghit::takingnotes:




I just bend some wire to shape and use electricians tape for a handle, no contams although I do throw it out & renew every few weeks (but this is due to rust rather than contams).


Oh and this thread is a couple of years old...


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InvisibleJuiceh
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: forestly]
    #18677614 - 08/08/13 11:49 AM (10 years, 7 months ago)

Quote:

forestly said:
Cool tek! The only so-so problem i see with it is that when you twist them together there are many spaces where contamination can hide. So sterilizing with alcohol would not be perfect. But if you sterilize this DIY loop in a PC then it would work as a charm.
:bonghit::takingnotes:



Inoculation loops, scalpels, and other metal tools like picks and tweezers should never just be sanitized with alcohol before use. Always flame sterilize. Way to bump and old thread to let everyone know you don't know that.
:goodluckwiththat2:


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Edited by Juiceh (08/08/13 11:54 AM)

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OfflineNakor420
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Re: OoBYCoO's Homemade Inoculation Loop TEK! [Re: Juiceh]
    #18677679 - 08/08/13 12:01 PM (10 years, 7 months ago)

I order the real thing cause I'm a myco snob. :wink:


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