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OfflineGonkulator
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Registered: 09/27/10
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Question about rye in agar
    #14143337 - 03/18/11 01:55 PM (12 years, 10 months ago)

I did a quick search and didn't find a lot about the subject, but of course there are always a few elusively dexterous threads hidden out in the database. Anywho, feel free to link me a thread if mine seems repetitively bothersome.

I was reading through someone's growlog of a Portabello grow, and the poster stated he / she felt as though their mycelium would slowly colonize when spawned to a bulk substrate, as if the organism was like "what is all this new food source?"

So, is this just an anthropomorphic (so to speak) expression given to the mycelium? Or do our mycelium actually become accustomed, as if in a routine, to breaking down grain "X" or substrate "Y"? If this is plausible, is it logical in any capacity to use sterilized grain, whether powderized or whole, in the agar? The thought is that if mycelium does, in a sense, "get accustomed" to the routine and / or mechanics of breaking down a substance, could we introduce this grain on agar so that colonization times are a bit faster when we use agar to inoculate a larger collection of grains?

Is this why folks take a particular affinity with grain-made LCs? If the mycelium in the liquid culture has been exposed to the same grains, it could possibly be more aggressive. Any thoughts?

Also, I realize the benefits, in a non-parochial scope of using agar / LC as a starting point for grain inoculation (no germination period / cleaning up a culture [in agar's case]). I was just speculating about a mycelia's potential to "recognize" a familiar source of food. Feel free to kick my noodlieboos if this question seems nonsensible. :p

edit: grammar


Edited by Gonkulator (03/18/11 01:57 PM)


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Offlinecrp32008
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Re: Question about rye in agar [Re: Gonkulator]
    #14143484 - 03/18/11 02:26 PM (12 years, 10 months ago)

Quote:

Gonkulator said:

So, is this just an anthropomorphic (so to speak) expression given to the mycelium? Or do our mycelium actually become accustomed, as if in a routine, to breaking down grain "X" or substrate "Y"? If this is plausible, is it logical in any capacity to use sterilized grain, whether powderized or whole, in the agar? The thought is that if mycelium does, in a sense, "get accustomed" to the routine and / or mechanics of breaking down a substance, could we introduce this grain on agar so that colonization times are a bit faster when we use agar to inoculate a larger collection of grains?




In short yes.  Mycelia use enzymes to break down certain materials.  When they are utilizing certain ones more than others they start producing more of the certain type.  This means that when they switch to a new source, they will have to switch which enzymes they produce more of.  This does cause a small hault but, then again when you transfere you'll have a recovery period anyway due to harming the mycelia in the transfere.  It isnt a noticable difference in most cases but when you refer to buttons/ports, they are slow as balls anyway haha

Quote:


Is this why folks take a particular affinity with grain-made LCs? If the mycelium in the liquid culture has been exposed to the same grains, it could possibly be more aggressive. Any thoughts?




No, this is not why people use glc.  They use that because they see thehealthy myc and know it is not contamed as opposed to going from spore to lc which makes it hard to see contams.  If it is spore to grain then grain to lc, u can see if its contamed or not.  However, you raise a great question about whether or not that is why the myc is more aggressive...I don't wanna answer that because I personally am not sure and don't wanna lead you in a wrong direction there.  glc though is an excellent tek...I do I it still when I have an iso'ed grain jar to save some lc in the fridge.

Quote:


Also, I realize the benefits, in a non-parochial scope of using agar / LC as a starting point for grain inoculation (no germination period / cleaning up a culture [in agar's case]). I was just speculating about a mycelia's potential to "recognize" a familiar source of food. Feel free to kick my noodlieboos if this question seems nonsensible. :p

edit: grammar




Mycelia recognize food sources in the sense that they change how much of one deomposing enzyme vs the other they have to produce.  Hope this helps.

Using powdered grain in agar (IMO) has too many nutrients in it.  I don't want my mycelia reproducing that much on agar because then they may (but also may not be) less vigorous in the future while doing g2gs or fruiting.  Plus, I don't want them fruiting on my dish.  This is a preference.  Im sure it's been tried and worked great.  I use to use brf in lc at one point and it worked fine. Im sure if you used it in agar it would work as well. Either way though when you cut teh myc to transfere it, youre harming it and it will have a day or so of recovery (this is my general observation).


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"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

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OfflineGonkulator
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Re: Question about rye in agar [Re: crp32008]
    #14144482 - 03/18/11 05:37 PM (12 years, 10 months ago)

Very educated feedback! Thank you!


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Offlinecrp32008
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Re: Question about rye in agar [Re: Gonkulator]
    #14144541 - 03/18/11 05:55 PM (12 years, 10 months ago)

Thanks bro!  Im glad it helped out:thumbup:  If you have any questions or anything lemme know


--------------------
"The truth is, no one really knows the truth. But, brief moment we may be able to taste it. This is the "a-ha!" moment of life. But then, just as quickly as it comes, it has left without warning. The lingering feeling of knowing, even for a second, what others are rarely open to leaves us thankful to have experienced it. Out of this comes a whole new look on life, ourselves, and others. The real human experience changes us forever. Even if all we catch is a glimps of it, to know it is there puts peace in our hearts and love toward our fellow man."

My Journal Entry


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