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OfflineOoBYCoO
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OoBYCoO's LC Lid & Brown Rice Syrup LC TEK
    #14139426 - 03/17/11 08:56 PM (12 years, 10 months ago)

Let's start w/ making an LC lid...

You need a jar and lid that can be PC'ed, I used a vaccutainer injection port that I removed from a test tube but you can use RVT silicone, and I used a filter patch from a used bag (after it was thoroughly cleaned and disinfected w/ bleach) but a Whattman filter plug is ideal.


OK now you need to make a hole in the lid for the injection port.  I start off using the smallest drill bit and gradually increase bit size till I get it to the desired size.  There's two reasons for this, first the thinner bits tend to be sharper and can make a cleaner hole in the metal lid w/o tearing the metal and ruining your lid like the duller thicker bits would.  Second, you don't want to accidentally make the hole too big.  I try to make it just big enough so I can barely fit the port in, making it snug and as tight a fit as possible.


Next, you need to make a hole for FAE...


You may also opt not to make an FAE hole...


Now you need to cut the filter patch to your desired size.  I cut mine in half, then took one of the halves and cut again in half giving me 2 squares which is what I'll be using (2 layers, one square on top of the other)...


It's time to get your silicone ready...


Put some of the caulk around the hole that you made for FAE, giving it a wide birth so as not to accidentally fill the hole w/ silicone (that would defeat the whole purpose).  Place the filter patch on, then put some more caulk lining the the perimeter of the square and place the second one on top.  Take some excess caulk and put some more going from the edge of the patch to the lid.  Also, put some caulk around the injection port where the port meets the lid (on both sides).


Let the silicone cure over night, the next day when you're ready to make the LC, clean it thoroughly before starting.  :grin:

-----------------------------------------------------------

So now you're ready to make the LC...

[My PC is busted so I'll be using a steamer]
Fill the pot w/ regular water and bring to a boil.  While the water is heating up, make your measurements to figure out how much water you will need for your jar.  Once the water is boiling turn off the heat and place the measuring cup w/ the DISTILLED water already measured out inside.


Use 1 teaspoon of brown rice syrup per 250ml of DISTILLED water.


Stir and mix it up.  The reason I do this is b/c the heat from the warm water outside of the measuring cup helps to dissolve the syrup into the distilled water.


Fill up your jar and place it in the steamer.  Bring the water to a boil again and let it sit for 90 min's.


Viola!!!  Brown Rice Syrup LC!!!  :awedance:


--------------------------------------------------------

Inoculating the LC...

I inoculate my LC's w/ agar wedges, but you could do it w/ a spore syringe.  OK so gather everything you're going to need/use and sterilize. [NOTE: These procedures should be done in front of a laminar flow hood or glove box.]


Loosen the lid, but don't open it yet.  [If you're using a spore syringe, you don't need to do this, just plunge the needle through the port... after you sterilized it of course (needle AND jar/lid).] 
Open up your plate of colonized agar and using your scalpel cut the desired sector.  It doesn't have to be a huge piece... you can even put 2 small pieces in if you want...


Try to do this step as quickly as possible w/ the least amount of air movement.  After you've put the wedge in, close the jar up tight and don't forget to label it...


----------------------------------------------------

Don't worry about the cloudiness, it's just the brown rice syrup.  After the myc starts to develop it will start to clear up b/c that's its food!!!  lol  This is what you'll end up with...


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Invisiblecyanara
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Registered: 12/22/09
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14139487 - 03/17/11 09:05 PM (12 years, 10 months ago)

very well done homie.:congrats:
Awesome write up.. you finaly did it..
Oh and like the pee cup btw:rofl:


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OfflineYogi Bear
Hey hey Bubu!?


Registered: 03/26/10
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14139698 - 03/17/11 09:38 PM (12 years, 10 months ago)

nice! I had this project lined up for next weekish, and now I dont even have to think while doing it :smile: Yahoo!


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The ranger isn't going to like this...


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OfflineGenesis311
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Re: Tobacco's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14139731 - 03/17/11 09:43 PM (12 years, 10 months ago)

i love the BRF LC idea.
it obviously works well for you and i love when people change up the rules a little bit and experiment and find out what works


--------------------

Please pardon any misspellings or grammatical errors, english is not my first language


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: cyanara]
    #14139935 - 03/17/11 10:05 PM (12 years, 10 months ago)

Thanks guys!

Quote:

cyanara said:
very well done homie.:congrats:
Awesome write up.. you finaly did it..
Oh and like the pee cup btw:rofl:



lol I know right!  Now I gotta go post it over there!  BTW I saw you asking ?'s in the IMF thread and that provoked me to finally get off my ass.  :grin:


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InvisibleBuddaking
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14140031 - 03/17/11 10:16 PM (12 years, 10 months ago)

:congrats: Nicely written & photographed Tek


--------------------







  AMU Q & A


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InvisibleBuddaking
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14140071 - 03/17/11 10:21 PM (12 years, 10 months ago)

Quote:

OoBYCoO said:
You may also opt not to make an FAE hole...






Have you ever noticed slowed growth with no GE hole compared to ones with hole ???


Edited by Buddaking (03/17/11 10:23 PM)


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: Buddaking]
    #14140086 - 03/17/11 10:23 PM (12 years, 10 months ago)

Thanks man!  I figured I'd do this up since there have been so many threads asking about it.  No one was ever really sure and the people that claimed they were going to try it never bothered to post their results, so I figured I'd solve it once and for all!  :grin:


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: Buddaking]
    #14140117 - 03/17/11 10:28 PM (12 years, 10 months ago)

Quote:

Buddaking said:
Quote:

OoBYCoO said:
You may also opt not to make an FAE hole...






Have you ever noticed slowed growth with no GE hole compared to ones with hole ???



To be honest, I'm never making a GE hole in my lids ever again.  First off, you don't really need it and secondly it just opens the door for possible contams, if whatever you're using (polyfil or filter patch) gets wet by the LC.  And b/c you don't want it to get wet, you can't really shake the shit out of your jars to break up the LC.  Also, I haven't noticed any beneficial results from having the GE hole.  :shrug:


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InvisibleBuddaking
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14140234 - 03/17/11 10:43 PM (12 years, 10 months ago)

I have been having a little trouble lately with contams in my lc & have been debating the no filter lid myself. Some of the problem was lazy-ness in my tek & I have solved that. I have never tried W/O a filter but maby I will do a side by side of GE vrs. sealed & decide for myself. If I find no or little difference I will eliminate another vector for contams.


--------------------







  AMU Q & A


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: Buddaking]
    #14140270 - 03/17/11 10:46 PM (12 years, 10 months ago)

Yup that's always the best way to learn... trial & error.


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Invisiblecyanara
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Registered: 12/22/09
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14141214 - 03/18/11 01:39 AM (12 years, 10 months ago)

lol I know right!  Now I gotta go post it over there!  BTW I saw you asking ?'s in the IMF thread and that provoked me to finally get off my ass.  :grin:



Glad I'm able to help motivate you.
Btw saw that you posted over there and heard your a french secret agent.:laugh2:


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Offlinejingus

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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14923868 - 08/15/11 12:59 AM (12 years, 5 months ago)

Quote:

OoBYCoO said:
Quote:

Buddaking said:
Quote:

OoBYCoO said:
You may also opt not to make an FAE hole...






Have you ever noticed slowed growth with no GE hole compared to ones with hole ???



To be honest, I'm never making a GE hole in my lids ever again.  First off, you don't really need it and secondly it just opens the door for possible contams, if whatever you're using (polyfil or filter patch) gets wet by the LC.  And b/c you don't want it to get wet, you can't really shake the shit out of your jars to break up the LC.  Also, I haven't noticed any beneficial results from having the GE hole.  :shrug:





This makes me happy to see these little plastic urine sample jars :laugh:

I made an LC in one of those a while back.  I went into the ER with a friend of mine and went to take a piss.  I saw those in the bathroom and had to take a couple, because they are the good plastic that you can pressure cook, and even better, they come pre-sterilized

The first time i made an LC in there, I left the seal on there saying it was kept sterile, and never opened it once.  To poke the hole, i just heated the needle and poked through it, then taped it sealed.  I only inoculated a few jars with it, though

I like the brown rice syrup, i'm gonna go make some of these. :super:


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: jingus]
    #14924526 - 08/15/11 06:15 AM (12 years, 5 months ago)

Let me know how it works for you!  :borat:


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OfflineGuernica
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: jingus]
    #14925976 - 08/15/11 01:02 PM (12 years, 5 months ago)

Does anyone else think it's hilarious that people outside this forum would be appalled upon finding a pisscup labeled "Penis Envy" filled with what looks to be a sticky white liquid suspended in water in a refrigerator?


--------------------
"...we're the unhappy inheritors of the consequence of 6000 years of unopposed dominator culture. and what have we got? a shattered atmosphere, toxified oceans, schizophrenic populations and mendacious politicians. this is not what our parents raised us for, folks."


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: Guernica]
    #14926662 - 08/15/11 03:25 PM (12 years, 5 months ago)

:rofldrunk:


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InvisibleVaeVictum
Sativa Cyborg


Registered: 06/01/11
Posts: 4,397
Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: OoBYCoO]
    #14926712 - 08/15/11 03:35 PM (12 years, 5 months ago)

:murray:

Awesome write up. Might try this some day.


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OfflineOoBYCoO
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Re: OoBYCoO's LC Lid & Brown Rice Syrup LC TEK [Re: VaeVictum]
    #14926729 - 08/15/11 03:38 PM (12 years, 5 months ago)

:grin:


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