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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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First LC attemp
#14137366 - 03/17/11 03:33 PM (12 years, 10 months ago) |
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About to take my shot at producing some LC. Have a needle I just purchased with Amazonian spores I will be using.
Im following the Beginner friendly liquid culture method I found on here, which uses Honey, a half pint jar. Steam sterilization in a pot.
So I will boil the jar and lid for 40 minutes. Then I will remove jar and leave 3/4 full of the boiling water in it while I add my honey. The tek states to use a table spoon but a lot of feedback says that is overkill. So i will use slightly less? Maybe a teaspoon insdead. Any advice?
Then i will wrap lid with foil, put back into boiling water for 30 minutes, with jar only half submerged. let cool over night and inject 2cc worth of spore from my need which will be flame sterilized.
After that put into a dark warm place, and swirl it once daily.
Questions
My basement is only around 70 degrees, the tek says 79-84. Will I be ok? And what simple method can I use to heat it?
All feedback appreciated.
-------------------- Pain is just weakness leaving the body
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: First LC attemp [Re: iSHACKA]
#14137419 - 03/17/11 03:43 PM (12 years, 10 months ago) |
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70 is perfect temp.
2 cc is way to much. I use 1/2 cc of MS spore solution for my LC's
doesn't need to be in the dark either. Indirect natural sunlight is good for myc
Don't start stirring it till you see colonization.
GL. I've never done a honey LC...just ELME...it's way easier IMO...
1g. ELME to every 250mL water.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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NMR.Xochipilli
Strangerer



Registered: 11/09/09
Posts: 272
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Re: First LC attemp [Re: iSHACKA]
#14137429 - 03/17/11 03:45 PM (12 years, 10 months ago) |
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Don't worry about the temp. difference, LC's colonize just fine at ambient room temp as long as it's reasonable. I'd put something to break up the myc. chunks in the jar otherwise they'll be hard to suck up into a syringe...a marble or piece of glass or something.
I hate measuring things with spoons, use a syringe and you can be precise with your measurements. LC's do best at 2-4% sugar concentration and honey is 15-20% water. So about 4mL's honey/ 100mL water. Some go stronger, some weaker...preference. I heat honey or karo in the microwave for a few seconds in a shot glass to make it easier to suck up into the syringe.
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iamnotadream
Enis the Penis


Registered: 12/05/10
Posts: 2,599
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Re: First LC attemp [Re: iSHACKA]
#14137434 - 03/17/11 03:46 PM (12 years, 10 months ago) |
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use two teaspoons.
keep it slightler under 4%
make sure not to have any holes in your lc jar. LC jars don't need GE. it's a great way to get contams, and nothing makes me sadder than a contaminated sugar LC
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NMR.Xochipilli
Strangerer



Registered: 11/09/09
Posts: 272
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Quote:
todlow said: 2 cc is way to much. I use 1/2 cc of MS spore solution for my LC's
agreed, spores like to stick to the walls of the syringe though so make sure to give it a thump or two before releasing the load
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Ajaxx
Amateur Mycologist



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Quote:
iamnotadream said: use two teaspoons.
keep it slightler under 4%
make sure not to have any holes in your lc jar. LC jars don't need GE. it's a great way to get contams, and nothing makes me sadder than a contaminated sugar LC
i micropore tape good enough to cover the hole?
i have 4 LCs i made (first LC attempt) and all 4 are lookin good cant wait to see if theyre contaminated or not.
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iSHACKA
Sick boy


Registered: 03/01/11
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Re: First LC attemp [Re: Ajaxx]
#14137471 - 03/17/11 03:55 PM (12 years, 10 months ago) |
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hey thanks for the input guys.
Will any honey from a grocery store do? Like the cheap stuff. as long as its light colored?
Also are you sure the LC does not need GE?
1 more question, how full should the jar be after I have inoculated it? Like is a little headspace ok?
-------------------- Pain is just weakness leaving the body
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: First LC attemp [Re: iSHACKA]
#14137479 - 03/17/11 03:56 PM (12 years, 10 months ago) |
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About that much. (It's a wide mouth pint jar)
No, it doesn't need GE, but I put Whatmans on mine for GE, so I can PC w/ the lid fully sealed.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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egodeathflux
Guttersnipe



Registered: 02/02/10
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Re: First LC attemp [Re: iSHACKA]
#14137482 - 03/17/11 03:57 PM (12 years, 10 months ago) |
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You should make silicone injection ports, well worth it for LC.
Honey works just fine on it's own, the paler the better, some honey will make the water cloudy. Acacia honey is a good one to look out for, super pale. I use about 3g of honey per 100ml.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Ajaxx
Amateur Mycologist



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Re: First LC attemp [Re: iSHACKA]
#14137504 - 03/17/11 04:02 PM (12 years, 10 months ago) |
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karo is cheap as fuck. thats what i used and its working.
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Doc_T
Random Dude




Registered: 03/06/09
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Re: First LC attemp [Re: Ajaxx]
#14137522 - 03/17/11 04:06 PM (12 years, 10 months ago) |
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Malt > honey > karo. Malt and honey make sediment, don't let it weird you out.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


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Re: First LC attemp [Re: Ajaxx]
#14137558 - 03/17/11 04:13 PM (12 years, 10 months ago) |
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I was actually just reading your WBS tek DocT
Thanks for the input. Is using a half pint jar too small?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




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Re: First LC attemp [Re: iSHACKA]
#14137581 - 03/17/11 04:18 PM (12 years, 10 months ago) |
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Half pint is great, put 100 mL in there.
Doc34 has a wbs tek. I think you'll get better results doing it like RR does rye in his video. But the grains are smaller, you don't soak or simmer as long. 4 hour soak, simmer about ten minutes or until al dente, steam dry. Wire mesh strainer is required for wbs.
-------------------- You make it all possible. Doesn't it feel good?
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egodeathflux
Guttersnipe



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Re: First LC attemp [Re: Doc_T]
#14137881 - 03/17/11 05:04 PM (12 years, 10 months ago) |
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I've never had sediment with Acacia honey unless I've added gypsum, it really is great stuff. The LC comes out almost completely transparent making it easy to spot any contams by eye, I find this cuts down on contam'd jars when skipping the agar stage. If it goes cloudy you know you have problems. A great advantage for noobz.
I've found every malt I've used to be sediment heavy, have done some trials with filtering the dissolved malt through a coffee filter for cleaner, prettier LCs. Worked pretty well, added honey too as I'm sure some nutrients are lost when filtering.
Not arguing Doc, just my 2 cents.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Oh, yeah. Malt makes mad sediment, be prepared.
-------------------- You make it all possible. Doesn't it feel good?
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iamnotadream
Enis the Penis


Registered: 12/05/10
Posts: 2,599
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Re: First LC attemp [Re: Ajaxx]
#14138081 - 03/17/11 05:32 PM (12 years, 10 months ago) |
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Quote:
Ajaxx said:
Quote:
iamnotadream said: use two teaspoons.
keep it slightler under 4%
make sure not to have any holes in your lc jar. LC jars don't need GE. it's a great way to get contams, and nothing makes me sadder than a contaminated sugar LC
i micropore tape good enough to cover the hole?
i have 4 LCs i made (first LC attempt) and all 4 are lookin good cant wait to see if theyre contaminated or not.
That'll work. As long as you have awesome sterile procedure you can get away with a lot of things I prefer not to have a hole at all because it's not needed, and I've never had a contamination.
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NMR.Xochipilli
Strangerer



Registered: 11/09/09
Posts: 272
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Re: First LC attemp [Re: iSHACKA]
#14138365 - 03/17/11 06:17 PM (12 years, 10 months ago) |
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Quote:
iSHACKA said:
Will any honey from a grocery store do? Like the cheap stuff. as long as its light colored?
It's gotta be in a bear shaped container.
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egodeathflux
Guttersnipe



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I use Rowse Acacia Honey. It's perfect, may be a UK only item, not sure. Any honey will work, though the purer the honey the less likely it is to caramelise/burn during PC'ing.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Stains Blue
Stranger

Registered: 01/17/11
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Re: First LC attemp [Re: Doc_T]
#14138570 - 03/17/11 06:46 PM (12 years, 10 months ago) |
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Quote:
Doc_T said: Oh, yeah. Malt makes mad sediment, be prepared.
Thats good though IMO. It gives the mycelium a surface to grow on. I have noticed faster culture times on sediment vs non sediment LC
Good luck!
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egodeathflux
Guttersnipe



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I just like a crystal clear LC with nothing but myc clouds in it, keeps my mind at ease. Nothing wrong with sediment though, don't want to convey the wrong idea.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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Stains Blue
Stranger

Registered: 01/17/11
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egodeathflux definitely lots of people love clear LC solution for that very reason. Its really not much of a discussion @ OP... Some prefer honey (easy to come by easy to use) Some prefer LME/DEX or just LME (Creates strong and January snot thick LC Cultures and is cheap) Some prefer Karo (Create a sediment free crystal clear LC where you can detect signs of abnormality)
Its comes down to preference really, You will have great success with all 3 mediums LC for me was also a Great stepping stone into the world of Agar as well.
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egodeathflux
Guttersnipe



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Hells yeah, LC>agar beats the shit out of spores/MS>agar.
-------------------- "Atrophic interludes weave through my life far too often, for me to fight the biggest enemies" "Standing on the corner of 5th and Vermouth"
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iSHACKA
Sick boy


Registered: 03/01/11
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Ok so what is the consensus,
Now much honey do I add? Sorry I dont want to use a needle to measure it out either. Im thinking one teaspoon for the 100ml the Doc said to fill up to.
Sound ok?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: First LC attemp [Re: iSHACKA]
#14139127 - 03/17/11 08:07 PM (12 years, 10 months ago) |
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I do it by weight, 4%. Put jar on scale, set zero, add four grams honey. Re-zero, add 100g water. Add marble or stirbar.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


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Re: First LC attemp [Re: Doc_T]
#14139167 - 03/17/11 08:12 PM (12 years, 10 months ago) |
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Ok well the tek im using. I have to add the honey after the water has been boiling
So one teaspoon of honey is 7 grams. Im gonna eye it out and use under one teaspoon for 100g water.
-------------------- Pain is just weakness leaving the body
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iSHACKA
Sick boy


Registered: 03/01/11
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Re: First LC attemp [Re: iSHACKA]
#14139389 - 03/17/11 08:49 PM (12 years, 10 months ago) |
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Ok
So I finished boiling my jar for 40 minutes. then i took the jar out, filled it about half full of boiling water, added slightly less then a teaspoon on honey, stired. put id on, foil over lid and no the jar is sitting in a pot with water boiling and a lid on. The jar is sitting directly on the pot. Stove is on low holding the boil.
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: First LC attemp [Re: iSHACKA]
#14139393 - 03/17/11 08:51 PM (12 years, 10 months ago) |
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Quote:
iSHACKA said: Ok well the tek im using. I have to add the honey after the water has been boiling
Wut?
-------------------- You make it all possible. Doesn't it feel good?
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Fungal growth
Lootinint



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Re: First LC attemp [Re: Doc_T]
#14139474 - 03/17/11 09:04 PM (12 years, 10 months ago) |
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i mix my lme into the water using my stirplate. i get very little sediment.
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DeadHearts


Registered: 07/17/09
Posts: 21,827
Loc: MICHIGAN
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Re: First LC attemp [Re: iSHACKA]
#14139538 - 03/17/11 09:16 PM (12 years, 10 months ago) |
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Quote:
iSHACKA said: Ok
So I finished boiling my jar for 40 minutes. then i took the jar out, filled it about half full of boiling water, added slightly less then a teaspoon on honey, stired. put id on, foil over lid and no the jar is sitting in a pot with water boiling and a lid on. The jar is sitting directly on the pot. Stove is on low holding the boil.
You are doing everything right. After the time is up just keep the lid on and let sit overnight.
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iSHACKA
Sick boy


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excuse my horrendos spelling and grammer Man i love this shit.
-------------------- Pain is just weakness leaving the body
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DeadHearts


Registered: 07/17/09
Posts: 21,827
Loc: MICHIGAN
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Re: First LC attemp [Re: iSHACKA]
#14139589 - 03/17/11 09:23 PM (12 years, 10 months ago) |
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Quote:
iSHACKA said: excuse my horrendos spelling and grammer Man i love this shit.
Its fun
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semaphore



Registered: 12/14/10
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I usually have a jar of about 250-280ml of water, i then put in about half a tablespoon of honey (in SA we get very good honey), i PC it for 20mins after about a week the LC go's clear with myc in it.
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iSHACKA
Sick boy


Registered: 03/01/11
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Re: First LC attemp [Re: semaphore]
#14142324 - 03/18/11 09:42 AM (12 years, 10 months ago) |
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Ok so a few post questions here
Just finished knocking it up. I used roughly 1cc of Amazonia pink elephant 
After knocking it up. I put the foil back over it. Shook the hell out of the jar.
The liquid in the jar is very very clear, it is only slightly hazy, also after shaking it, it did not apear to become frothy. Is this normal?
As of know its sitting on top of a shelf, with only foil over it (and the jar lid) So there is an open hole that is covered by two laters of foil.
Sound good?
-------------------- Pain is just weakness leaving the body
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Matty_Rulez
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Re: First LC attemp [Re: iSHACKA]
#14142357 - 03/18/11 09:54 AM (12 years, 10 months ago) |
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Sounds pretty good... how many holes in the lid??
I put 2 small holes in mine... cover one with a blob of silicone, and the other i put micropore tape over... when I want to use some LC, i just remove the micropore tape, cover the hole with a gloved finger, and shake the shit out of it... breaks the myc up nicely.. then I just tip it upside down, and draw some LC in through the self-healing silicone injection port...
I've tried the baby bottle TEK, but I prefer this way...
my 2 cents...
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
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iSHACKA
Sick boy


Registered: 03/01/11
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Oh nice a fellow Canadian. Toronto by any chance?
-------------------- Pain is just weakness leaving the body
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Matty_Rulez
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Re: First LC attemp [Re: iSHACKA]
#14142386 - 03/18/11 10:04 AM (12 years, 10 months ago) |
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Nation's capital, baby! lol!
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
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Ajaxx
Amateur Mycologist



Registered: 03/06/11
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i didnt want to make a new thread since this is already a "first LC" thread
i did 700ml distilled water to a little under an ounce of karo. 4 jars total, and split up what little PF albino spores i had into each one. theyre doing great. clouds of mycelium are floatin around the bottom, all 4 have about the same amount in them.
is this too big of an LC? hopefully thats not a stupid question... im guessing theres nothign wrong with doing an LC that big, it just takes longer for it to fully colonize. and do i wait until the entire jar is a cloud of mycelium before testing out for contaminants? (im guessing i do)
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Matty_Rulez
Lurker, explorer



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Re: First LC attemp [Re: Ajaxx]
#14142484 - 03/18/11 10:29 AM (12 years, 10 months ago) |
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Quote:
Ajaxx said:
is this too big of an LC? hopefully thats not a stupid question... im guessing theres nothign wrong with doing an LC that big, it just takes longer for it to fully colonize. and do i wait until the entire jar is a cloud of mycelium before testing out for contaminants? (im guessing i do)
Hey there... as long as there's a good enough cloud of myc, you can test it... I have a Cambodian honey LC that i tested a week after dropping some spores into it... the LC is crystal clear now, so I have it in the fridge now...
Not too big at all...
Here's a pic of the Cambodian test jar I nocc'd up about a week and a half ago...
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
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iSHACKA
Sick boy


Registered: 03/01/11
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Hey guys
quick question,
My Jar is extreamly clear, all the pictures of jars I see posted are always yellowish? Could it be that my honey was just very very clear?
I used 3/4 of a table spoon, which i estimate was 4 grams. Into a 250ml half pint, filled half way.
thanks,
-------------------- Pain is just weakness leaving the body
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quebus
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Re: First LC attempt [Re: iSHACKA]
#14144000 - 03/18/11 04:07 PM (12 years, 10 months ago) |
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I posted a pic of mine elsewhere - clear as a bell with honey after a week. The again it was always clear. My honey container had a pour spout so no spoon or needle. Here it is again.
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iSHACKA
Sick boy


Registered: 03/01/11
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Re: First LC attempt [Re: quebus]
#14144030 - 03/18/11 04:14 PM (12 years, 10 months ago) |
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Ok i guess some honey simply is very clear.
-------------------- Pain is just weakness leaving the body
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Matty_Rulez
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Re: First LC attempt [Re: iSHACKA]
#14144179 - 03/18/11 04:39 PM (12 years, 10 months ago) |
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My honey LC had a slight "honeyish?" color when i innoc'd, but a week and a bit later... it was crystal clear... I've read this is the myc eating up all of the sugars...
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
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iSHACKA
Sick boy


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How much honey did you use?
-------------------- Pain is just weakness leaving the body
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Matty_Rulez
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Re: First LC attempt [Re: iSHACKA]
#14144200 - 03/18/11 04:42 PM (12 years, 10 months ago) |
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I used 4ml to 250ml tap water..
-------------------- Life would be tragic if it weren't funny. - Stephen Hawking
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Phoenix Crash
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I just started using LCs a few months ago, and I must say that the honey has been the best, by far. It's never really 'clear', just kinda looks like honey tinted water. I use a hair under a teaspoon of honey into 100mL of water that has been in the microwave for 1 minute. Stir it up, seal it up, and steam sterilize for 35 minutes. Leave the pot covered and noc it up in the morning, or after it's completely, 100% cool. Store it at 80-82F and you should see myc in less than a week. It seems to grow better in honey for some reason. The growth in Karo is half as fast as honey, and the ELME clouds up like a bitch. Just my though.
-------------------- Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks
Phoenix Crash LC Tek DIY Magnetic Stir Bars Tek "Not all drugs are good, all right? Some of them… are fuckin' great. Just gotta know your way around them, is all."
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iSHACKA
Sick boy


Registered: 03/01/11
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When is a good benchmark to assume to see growth? It seems like growth should happen quick if its to be done in only 7 days. So far I dont see anything.
-------------------- Pain is just weakness leaving the body
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: First LC attempt [Re: iSHACKA]
#14155040 - 03/20/11 05:39 PM (12 years, 10 months ago) |
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depending on what you use to noc up the LC
if using an agar wedge, or a section of a mushroom you can see growth in 2-3 days, if from spores it can take a week or longer to see first signs of growth.
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iSHACKA
Sick boy


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Re: First LC attempt [Re: biologys]
#14155258 - 03/20/11 06:19 PM (12 years, 10 months ago) |
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oh, it was from spores. That makes sense then, that I have not seen anything happen.
-------------------- Pain is just weakness leaving the body
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