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Offlinegroovydan
Popcorn teknewbie

Registered: 03/27/03
Posts: 11
Last seen: 13 years, 8 months
popcorn tek- healthy kit grain to 'corn innoc, need help!
    #1413189 - 03/27/03 05:36 AM (13 years, 8 months ago)

ive prepared the popcorn tek and followed all processes, instead of boiling i pc'd the corn, the pc'd two of the three jars.

ive innoculated the jars using donor grain from a very healthy and sucessful grow kit (that is pinheading).

the two jars i pc'd dont seem to be doing too well. (no 1 and 2)
the jar i didnt pc (because it huge) seems to be doing better. (no 3)

this morning no3 seemed to be doing well with good myce growth so ive shook it and left.

no1 and 2 didnt seem to be doing that well compartively and have added extra grain to them. i also added about two teaspoons of water to no2 because it seemed like it needed some extra hydration.

in 1 and 2 on some of the grain (that was pure white) it seems to have gone a dark green.

the corn in both doesnt seem as bright yellow as no3.

its only been two days since innoc and fear the worst situation- contams.

im a relative newbie but have read TMC over and over and really do firmly believe i understand the process of the mushroom cycle fully.

can someone shed some light? many thanks people
Dan


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: groovydan]
    #1413244 - 03/27/03 06:04 AM (13 years, 8 months ago)

did you pc the jars after you loaded corn into them?

did you add non-sterile grains to a sterile jar?

did you open the jars in a non-sterile environment while adding grain?


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Offlinegroovydan
Popcorn teknewbie

Registered: 03/27/03
Posts: 11
Last seen: 13 years, 8 months
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: Bilge]
    #1413299 - 03/27/03 06:30 AM (13 years, 8 months ago)

1) yes for 60mins at 15psi
2) potentially yeas, they were from the kit
3) kind of- in the tank im using to grow my kits. however i wore a clean beanie, rubber gloves, safety specs, mask and washed my upperarms, didnt breath over or into tank- breathed out over my shoulder away from everything


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Offlinegroovydan
Popcorn teknewbie

Registered: 03/27/03
Posts: 11
Last seen: 13 years, 8 months
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: groovydan]
    #1413361 - 03/27/03 07:06 AM (13 years, 8 months ago)

<a href="https://files.shroomery.org/files/032303-8/19641-popcorn_tek,_poss_contam_rye2.jpg" target="_blank"><img src="/forums/thumbs/032303-8/19641-thumb_popcorn_tek,_poss_contam_rye2.jpg"></a>

contam or bruising from shaking?

<a href="https://files.shroomery.org/files/032303-8/59160-popcorn_tek,_healthy_mycelium2.jpg" target="_blank"><img src="/forums/thumbs/032303-8/59160-thumb_popcorn_tek,_healthy_mycelium2.jpg"></a>

good mycelium, same jar


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: groovydan]
    #1413409 - 03/27/03 07:30 AM (13 years, 8 months ago)

hm, i'd wait and watch. some people have gone to boiling the corn a bit first but i'm not aware of anyone who pc'd it first. don't know what effect that would have except i would expect much more water content in the kernels after pc'ing.

but all in all it may be a little too early to make a contam determination. give it another few more days or a week?


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Offlinesi1988crx
Beep beep byebye

Registered: 03/08/03
Posts: 267
Last seen: 4 years, 11 days
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: groovydan]
    #1413466 - 03/27/03 07:49 AM (13 years, 8 months ago)

You most likely have contams. If you added non sterile grain to a jar and didnt pc after wards, no matter how clean you are their will be mold spores introduced into the jars. Next time, follow the directions as they are written and you wont have these problems.

You should let the corn soak overnight, then drain and load into the jars and pc for 60-90 min at 15 psi. After that let them cool down and innoculate. DO NOT open the jars for any reason whatsoever after that. It will take a FEW days before you will see growth of mycelium on all the jars. BE PATIENT. Also when you are doing g2g (grain to grain) transfers, you need to be extremely sterile and it is a good idea to use a flow hood.


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Offlinegroovydan
Popcorn teknewbie

Registered: 03/27/03
Posts: 11
Last seen: 13 years, 8 months
Re: popcorn tek- healthy kit grain to 'corn innoc, need help! [Re: si1988crx]
    #1413570 - 03/27/03 08:25 AM (13 years, 8 months ago)

the grain i added was colonised with mycelium from a very healthy grow kit i have. this is due to the lack of mushrooms to get a spore print to innoculate the corn that way.

if i pc'd the jars with grain i would have killed the mycelium.....


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