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Offlinevelvetmagician
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How long to flame sterilize syringe * 1
    #14126128 - 03/15/11 05:24 PM (12 years, 10 months ago)

I have went through the search function and could not find anything relating to my question. How long do I keep the syringe in flame to sterilize using a cigarette lighter? And, does the black soot matter?


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OfflineProfessorPinHead
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Re: How long to flame sterilize syringe [Re: velvetmagician]
    #14126155 - 03/15/11 05:27 PM (12 years, 10 months ago)

Until it is red hot. No the soot won't matter. We answer noob Q's here bro. In case you have any more that pop in your head.


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Offlinebakenast
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Re: How long to flame sterilize syringe [Re: velvetmagician]
    #14126168 - 03/15/11 05:28 PM (12 years, 10 months ago)

Quote:

velvetmagician said:
I have went through the search function and could not find anything relating to my question. How long do I keep the syringe in flame to sterilize using a cigarette lighter? And, does the black soot matter?




get it red hot if u can, if not its still fine,, and for black silt, I always get my syringe hot then swab with alchol soaked cotton ball before I poke needle in for inoculation, so it takes silt off, maybee just spend 9$ on a alchol lamp there soo handy!


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Offlinevelvetmagician
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Re: How long to flame sterilize syringe [Re: ProfessorPinHead]
    #14126169 - 03/15/11 05:28 PM (12 years, 10 months ago)

Nice. Thanks professor!


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Q: How many Micheal Jackson's does it take to screw in a light bulb?
A: NONE, he screws kids!


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Offlineshroomtastic89
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Re: How long to flame sterilize syringe [Re: velvetmagician]
    #14126215 - 03/15/11 05:39 PM (12 years, 10 months ago)

don't wipe it with a cotton ball after heating it up... This will just contaminate it again alcohol or not...


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Offlineunderfliptown
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Re: How long to flame sterilize syringe [Re: shroomtastic89]
    #14126227 - 03/15/11 05:41 PM (12 years, 10 months ago)

When I used my syringe i had already squeezed out the air bubble before sterilizing. I think there must have been some liquid inside the actual needle because it made a loud popping sound. At first i thought I had wrecked my syringe but it turned out to be ok.


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InvisibleShr00mZ
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Re: How long to flame sterilize syringe [Re: underfliptown]
    #14126301 - 03/15/11 05:58 PM (12 years, 10 months ago)

Quote:

underfliptown said:
When I used my syringe i had already squeezed out the air bubble before sterilizing. I think there must have been some liquid inside the actual needle because it made a loud popping sound. At first i thought I had wrecked my syringe but it turned out to be ok.





Anytime there is moisture in the needle; heating it up will cause a popping noise because its boiling the water. As long as your not burning the syringe itself your okay. Needles are metal... one can use them and re-use them many times.


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Offlinebakenast
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Re: How long to flame sterilize syringe [Re: shroomtastic89]
    #14126366 - 03/15/11 06:11 PM (12 years, 10 months ago)

Quote:

shroomtastic89 said:
don't wipe it with a cotton ball after heating it up... This will just contaminate it again alcohol or not...




disagree ive always does wiped with alchol after flame, cools tip down And sterilizes for quick innoc, sometimes i dont even bother with flame just straight alchol, and rarely have contams


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OfflinePsilocyentist
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Re: How long to flame sterilize syringe [Re: bakenast]
    #14127301 - 03/15/11 09:02 PM (12 years, 10 months ago)

Quote:

bakenast said:
Quote:

shroomtastic89 said:
don't wipe it with a cotton ball after heating it up... This will just contaminate it again alcohol or not...




disagree ive always does wiped with alchol after flame, cools tip down And sterilizes for quick innoc, sometimes i dont even bother with flame just straight alchol, and rarely have contams




This is really bad advice. You encourage sticking a non sterile object into a sterile environment? Alcohol does not sterilize, and any contams on your needle have a very good chance of taking hold of your sterile substrate before your mycelium. Just wanted to address this so no newbies get confused.


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Offlineshroomtastic89
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Re: How long to flame sterilize syringe [Re: bakenast]
    #14129866 - 03/16/11 10:06 AM (12 years, 10 months ago)

Quote:

bakenast said:
Quote:

shroomtastic89 said:
don't wipe it with a cotton ball after heating it up... This will just contaminate it again alcohol or not...




disagree ive always does wiped with alchol after flame, cools tip down And sterilizes for quick innoc, sometimes i dont even bother with flame just straight alchol, and rarely have contams





Well imagine how many contams you would have had if you didn't use the alcohol swab after you flame...


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OfflineRogerRabbitM
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Re: How long to flame sterilize syringe [Re: shroomtastic89] * 1
    #14129940 - 03/16/11 10:23 AM (12 years, 10 months ago)

Flame sterilize until red-hot, and NEVER wipe down with a towel afterward, whether it has been alcohol soaked or not.  Anyone who thinks alcohol will sterilize, need only dip their thumb in alcohol and then touch it to a petri dish.  within a few days, you'll see a perfect thumbprint of mold and bacteria growing.
RR


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Offlinebakenast
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Re: How long to flame sterilize syringe [Re: RogerRabbit]
    #14131389 - 03/16/11 02:27 PM (12 years, 10 months ago)

Quote:

RogerRabbit said:
Flame sterilize until red-hot, and NEVER wipe down with a towel afterward, whether it has been alcohol soaked or not.  Anyone who thinks alcohol will sterilize, need only dip their thumb in alcohol and then touch it to a petri dish.  within a few days, you'll see a perfect thumbprint of mold and bacteria growing.
RR




haha good point maybee none Idk I just always did that maybee Ill switch it up:smile:


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OfflineProfessorPinHead
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Re: How long to flame sterilize syringe [Re: bakenast]
    #14131848 - 03/16/11 03:47 PM (12 years, 10 months ago)

Quote:

bakenast said:
Quote:

RogerRabbit said:
Flame sterilize until red-hot, and NEVER wipe down with a towel afterward, whether it has been alcohol soaked or not.  Anyone who thinks alcohol will sterilize, need only dip their thumb in alcohol and then touch it to a petri dish.  within a few days, you'll see a perfect thumbprint of mold and bacteria growing.
RR




haha good point maybee none Idk I just always did that maybee Ill switch it up:)



One would hope so, :yoda:


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OfflineSolil8quy
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Re: How long to flame sterilize syringe [Re: ProfessorPinHead]
    #27829341 - 06/20/22 09:24 PM (1 year, 7 months ago)

Does this mean until the needle is visibly red????!??!
I have a frickin overpowered light, don't want to do overdo it.


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InvisibleBlazer420
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Re: How long to flame sterilize syringe [Re: Solil8quy] * 1
    #27829392 - 06/20/22 09:47 PM (1 year, 7 months ago)

yes when it glows red it is fine


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