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scriptlord666
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Registered: 06/16/10
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Last seen: 12 years, 3 months
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Strange happenings with pf tek jars
#14125703 - 03/15/11 04:12 PM (12 years, 10 months ago) |
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Ok maybe 2 months ago i did my first attempt using a pressure cooker and making my own jars. I made 4 jars one day, innoccd and the next day made 4 more. I used a good bit of vermiculite for the top layer, i'm thinking maybe too much. I also used about 5 layers of tin foil one on the top the other to help prevent contam. I normally use an incubator but couldn't this time. They were probably at an average of 70 degrees. After 15 days there was no noticeable growth in any. So i put them aside and gave up just never getting rid of them. 2 months or so later going through old things I look and see everyone of them has colonized and none have infections. The top tin foil came down to about half of the half pint jars ( i didn't use wide mouth btw). well when i open the jars the bottom half are beautifully colonized and a large portion of the top half are not colonized at all but not infected just wet and uncolonized tho the bottom is perfectly colonized. I birthed the jars and dunked them and used a sterile knife to cut off the uncolonized parts and put them in a shotgun fc and tho it was only a few days ago i don't see any problem with them being completely fine. i just wonder why the top halves didn't colonize. i also am glad to say out of 8 jars i had zero contaminations and this was my first attempt at pf tek thankyou, shroomery is awesome scriptlord
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: Strange happenings with pf tek jars [Re: scriptlord666]
#14125846 - 03/15/11 04:42 PM (12 years, 10 months ago) |
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You dont need that many layers of aluminum foil.. 2 is fine. The part thats not colonized, could that be your verm layer? (since you said you added a bit more then normal) Pics always help. And hit the enter key every now and then. 
Goodluck
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scriptlord666
Stranger

Registered: 06/16/10
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Last seen: 12 years, 3 months
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Re: Strange happenings with pf tek jars [Re: esquaredx]
#14126203 - 03/15/11 05:35 PM (12 years, 10 months ago) |
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it seems to be more than the verm layer. its like half the jar completely. but again no contams and its just fine like it didn't colonize. i cut it off and put it in the shotgun fc. i just can't figure out why the top half wouldn't colonize. and it was all the top half. would a 1 inch layer of verm possibly make like 2 inches not colonize? it also seemed to be like when the foil covered the top half, could that have any effect? i mean all and all i consider this a success and i should continue on my attempts right? anyone have any previous experiences of this happening?
thankyou again
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: Strange happenings with pf tek jars [Re: scriptlord666]
#14126219 - 03/15/11 05:40 PM (12 years, 10 months ago) |
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Could be a number of reasons it didnt finish. What kind of lids do you use?(do they have GE holes) Did you leave the foil on even after inoculation? Maybe your brf was packed down to tight.?
Use that enter button, it makes it alot easier to read.
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scriptlord666
Stranger

Registered: 06/16/10
Posts: 38
Last seen: 12 years, 3 months
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Re: Strange happenings with pf tek jars [Re: esquaredx]
#14130542 - 03/16/11 12:39 PM (12 years, 10 months ago) |
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i used the lids that came with the ball jars. btw so leaving tin foil on after inoculation could of caused it? should i leave any layers of tin foil on, like just one? i plan on putting like the five layers on during the time in the pressure cooker but so after that i should remove all but one layer? or remove them all?
thanks for any input
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: Strange happenings with pf tek jars [Re: scriptlord666]
#14130596 - 03/16/11 12:46 PM (12 years, 10 months ago) |
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Use two layers of foil while pressure cooking, no need at all for more then that. Do you have holes drilled in your lid? do you cover them with tape, RTV, polyfil? anything? After you take them out of the pressure cooker, leave the foil on until you inoculate the jars. Then keep the foil off.. You do have a dry verm layer correct?
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scriptlord666
Stranger

Registered: 06/16/10
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Last seen: 12 years, 3 months
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Re: Strange happenings with pf tek jars [Re: esquaredx]
#14130825 - 03/16/11 01:14 PM (12 years, 10 months ago) |
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yeah i have a dry verm layer. i don't use any tape at all i just used the tin foil. so use two layers for the pressure cooker, then innocc and take all the layers off and they will be fine with just the verm layer and the open hole? should i put any breathable tape over it or anything? i don't drill my holes i just prepunched them with a small nail just big enuf for the needle tip. i even did it after they had been innocd cuz the hole wasn't big enuf and i still had no contams. anyways any more advice or help is appreciated.
thankyou,
scriptlord
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: Strange happenings with pf tek jars [Re: scriptlord666]
#14130872 - 03/16/11 01:21 PM (12 years, 10 months ago) |
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Okkay. So if youd like, you can use micropore tape before pressure cooking (help with moisture that may get in). But theres no need for tape after you pc. Just take the foil off when your ready to shoot the jars up. Shoot em up and set them somewhere, and dont play with the jars. The holes will be fine, as long as the dry verm layer is intact and not wet or anything.
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Psilocyentist
Carbon based


Registered: 11/04/10
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Re: Strange happenings with pf tek jars [Re: esquaredx]
#14131277 - 03/16/11 02:11 PM (12 years, 10 months ago) |
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I'd bet it was the foil that stalled your jars, hindered Gas Exchange.
-------------------- I know, I know you probably scream and cry That your little world won't let you go
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: Strange happenings with pf tek jars [Re: Psilocyentist]
#14131689 - 03/16/11 03:18 PM (12 years, 10 months ago) |
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Quote:
Psilocyentist said: I'd bet it was the foil that stalled your jars, hindered Gas Exchange.
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scriptlord666
Stranger

Registered: 06/16/10
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Last seen: 12 years, 3 months
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Re: Strange happenings with pf tek jars [Re: esquaredx]
#14137547 - 03/17/11 04:11 PM (12 years, 10 months ago) |
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i have a strange habit of likeing to inject thru one or two layers of foil for some reason i thought it just helped more with avoiding contam. so basically, do everything the same, EXCEPT after innoc take the foil off (should i put any micropore tape on?) and just leave them be. i just i dunno feel a little paranoid with that exposed hole but i guess thats what faith and the dry layer of verm is there for. shroomery rules yet again thankyou so much....with the help of shroomery in a few months i went from presterilized jars that over 50 percent failed, to presterilized jars with 0 percent failure out of 50 or more, to making my own and 0 contams on my first two attempts. tho may not be the best success with only half colonization i'm still proud, and i believe they just started pinning today. oh they grow up so fast...
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