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OfflineLarrythescaryrex
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substitute Petri Dishes
    #14122800 - 03/14/11 11:22 PM (12 years, 11 months ago)

so Doc T and I were discussing this in the pub like two weeks ago, and if I recall correctly he said he has just been using jars for petri dishes. Now, If I were to attempt this, would I be best served by using Parafilm to cover them, or simple a sterilize jar lid? Also, assuming sufficient agar content, how long of a storage period, in the fridge, could one expect. At this point it would basically be a big slant right?


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RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011

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Invisibleschmoopy
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Re: substitute Petri Dishes [Re: Larrythescaryrex]
    #14123004 - 03/15/11 12:01 AM (12 years, 11 months ago)

I recently used this grocery store agar tek with success.
I omitted the tyvek and put jar lids upside down.

Left them covered with foil in the fridge for a week before using them.
I bet they would do fine with more long term storage as long as they are covered well.

They had some condensation which made viewing tricky, but were successfully sterile and workable for me. They just take up a lot of room.


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The observer is the observed.
-J. Krishnamurti

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InvisibleLeopardMan
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Re: substitute Petri Dishes [Re: Larrythescaryrex]
    #14123638 - 03/15/11 03:37 AM (12 years, 11 months ago)

You can use small jars, they work fine if you need to do some agar work. Obviously petri dishes work better though.
If you want to store cultures for more than a year, buy culture tubes and add a small piece of wood to your agar (RR uses tongue depressors).


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OfflineMicroppose
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Re: substitute Petri Dishes [Re: LeopardMan]
    #14123639 - 03/15/11 03:39 AM (12 years, 11 months ago)

I wonder if jello shot cups would work, they lack surface area, but if you can work around that I don't see why it would be a problem.

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Invisiblesandman420
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Re: substitute Petri Dishes [Re: Microppose]
    #14124512 - 03/15/11 10:45 AM (12 years, 11 months ago)

i use baby food jars. 2 jars fit inside a ziplock baggy so you dont need any parafilm. I pc the empty baby food jars in a spawn bag with the agar. Then I pour em and put 2 per ziplock in front of a flowhood. So easy.


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OfflineCloneufc
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Re: substitute Petri Dishes [Re: sandman420]
    #14124532 - 03/15/11 10:49 AM (12 years, 11 months ago)

Just drill a small hole in the lid and place micropore tape over the hole. PC the whole jar with the lid on it, pour the agar and then screw the lid back on. I do this when I run out of petri dishes or if I need to clean up a badly contaminated culture that will require many transfers.

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InvisibleDoc_T
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Re: substitute Petri Dishes [Re: Cloneufc]
    #14125168 - 03/15/11 02:19 PM (12 years, 11 months ago)

No need for holes. :smile:

An undrilled plastic lid, or an undrilled metal lid turned upside down, will work great for agar jars.

Also, pour agar into jars and then PC it. Easy.
Or you can store it cold in the fridge, slice pieces into the jars, and PC.


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OfflineLarrythescaryrex
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Re: substitute Petri Dishes [Re: LeopardMan]
    #14127544 - 03/15/11 09:45 PM (12 years, 11 months ago)

whats up with the wood thing?


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011

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Invisiblemaiko maiko
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Registered: 02/26/10
Posts: 332
Re: substitute Petri Dishes [Re: Doc_T]
    #14127611 - 03/15/11 09:54 PM (12 years, 11 months ago)

Quote:

Doc_T said:
pour agar into jars and then PC it. Easy.




:thumbup:

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