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Invisiblecyanara
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1st backup slants attempt.
    #14119351 - 03/14/11 01:07 PM (13 years, 2 months ago)

Ok so I have never done this and am about to attempt my first back up tubes. So I have lab grade malt extract, should I use as directed or dilute it down?
Also if I'm doing the backups like RR says with wood in the tube, could I use those cool wooden coffee stir stix from starbucks intsead of buying medical tongue depressors? casue I'm pretty sure they are made from birch....
any advice would be greatly appreciated.

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Offlineclueless
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Re: 1st backup slants attempt. [Re: cyanara]
    #14120062 - 03/14/11 03:53 PM (13 years, 2 months ago)

The wooden swizzle sick will work. I'd mix a standard mea mix. Fast Fred's Media Cookbook is a good reference if you need recipes.

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: clueless]
    #14120444 - 03/14/11 05:05 PM (13 years, 2 months ago)

cool. I got this deliciouse lab grade MEA
says per liter of water.....
15g Agar
20g Malt
20g Dextrose
6g Pepton
and adjusts to 5.5 ph when done and at a nice 25C.
can't wait, this is gona be fun, i feel like a kid on xmas

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14120484 - 03/14/11 05:11 PM (13 years, 2 months ago)

That seems really nutritional for slants. I use just agar and water with a wooden stick in it. It acts almost the same way as suspending mycelium in distilled water for long term storage. The lack of nutrients causes the mycelium to slow down its processes and allow for a longer storage time.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14120558 - 03/14/11 05:22 PM (13 years, 2 months ago)

ya, I just got this 1lbs bottle of lab MEA, I know stamets recomends 1/2 the ammount of malt for tube storage, I know i've asked a few times and RR said just to use the regular stuff, so..
I't my first time so chances are I'll fuck up something..

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14120601 - 03/14/11 05:30 PM (13 years, 2 months ago)

Well if your looking for easy long term storage, you should just store in distilled water. I have recently started working with cryogenicly  freezing mycelium in a glycol solutions. I got my fingers crossed.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14120865 - 03/14/11 06:14 PM (13 years, 2 months ago)

long term storage, hopefuly. right now I just realy want to preserve my genetics. when I whent to the science store they always have this discount rack, and they had these pre-sterilized drahm like vials with screw cap and injector ports... should of gotten some or at least asked the guy if they where autoclavable.. perfect for LC and water suspension.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14136909 - 03/17/11 01:49 PM (13 years, 2 months ago)

Hi cyanara; that is an overly rich medium. A standard MEA is 30gr DME and 15gr agar in to a liter of water. The medium that RR uses is from Fungi Perfecti which is Paul Stamets company and is 20gr DME, 20gr agar, 2gr yeast extract and 1gr soy peptone. This is the one I would recommend using.

You might want to uses just 30gr of your MEA powder instead of 61gr plus 10gr of agar to a liter of water.

Edited by CheeWiz (03/17/11 04:19 PM)

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: CheeWiz]
    #14136983 - 03/17/11 02:11 PM (13 years, 2 months ago)

word...

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14167916 - 03/23/11 12:37 AM (13 years, 2 months ago)

Quote:

TheShroomJew23 said:
That seems really nutritional for slants. I use just agar and water with a wooden stick in it. It acts almost the same way as suspending mycelium in distilled water for long term storage. The lack of nutrients causes the mycelium to slow down its processes and allow for a longer storage time.



how much growth do you get on just agar with a wooden stick?

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Re: 1st backup slants attempt. [Re: cyanara]
    #14168969 - 03/23/11 09:48 AM (13 years, 2 months ago)

slants are tricky, and you will do better the more you try. RR's trick with the little piece of wood is the word, I tried that during last winters slant lab and I have never had better success. Stametes IN GGMM states that he doubles the agar for firmness. I tried that and found the result to be too firm. so I go with 15g of agar per 500 ml of solution. He also states that the agar mix can be essentially non nutritive, which I agree with. It encourages the myc to run the slant and then shut down. The little piece of wood is nut enough, and when I start pulling on last winters slants a year from now I will pull the wood, clip off a little piece from the end and drop onto a plate.

I have a stir table that I mix for a long period of time my agar solution to ensure a thorough dissolve of the agar. I usually make up my mix in a 1000ml beaker and then go to have breakfast and a shower before coming back, and transferring the agar into the slants and PCing

:goodluck:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14169072 - 03/23/11 10:14 AM (13 years, 2 months ago)

thanks man, I finaly have some good genetics and don't want to loose them.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14171133 - 03/23/11 04:20 PM (13 years, 2 months ago)

if my house caught on fire, once my dog was out I would go for the slant cooler in the fringe and my 2tgig external hard drive, which are in a comparable way the same thing. Genetics are Irreplaceable, once lost they can never be recovered in most cases. I have lost a few wild clones in the process of learning slants, but nothing that I cared for more than a second that I lost, some wild oysters, etc.

Good luck with your process.


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14171293 - 03/23/11 04:49 PM (13 years, 2 months ago)

thanks man. I'm guessing you have alot of good music on that external?
Do you have experience with bags.

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Invisiblemycoelf
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Re: 1st backup slants attempt. [Re: cyanara]
    #14171913 - 03/23/11 06:44 PM (13 years, 2 months ago)

tons of music, myco data, etc. Irreplaceable notes and research. Lots of pics of my work.


Yes, bags is all that I do, essentially for everything, IMHO they are the only way to fly unless you are doing something really exotic like cordyceps


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14171943 - 03/23/11 06:48 PM (13 years, 2 months ago)

can you explain your procedure on prepping grains for bags, cause I'm getting into that as well as upgrading my equipment and getting an AA 75X, so I'm trying to educate myself before I transition.
Ya, I would loose my shit if I lost all of my music. I spin digital, and besides my wife and this, it's everything to me.. lucky for me I have a cat so I can grab my laptop and cultures while she makes it out ok.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14172079 - 03/23/11 07:10 PM (13 years, 2 months ago)

I prefer to use both white and red millet, just cause it is cheap and available, white millet I like for G1 because the color makes contams easy to spot. White millet must be simmered + soaked to get the very tough seed hulls to open up, facilitating the myc's entry. I usually simmer to almost a boil and let sit overnight, followed by a severe tap water rinse. I usually mix in a little verm to facilitate H20 balance, then you mix your gypsum and load into your jars/bags.

I favor red millet for sawdust supplementation, you can cold soak over night and the grains swell easily. The water turns red, again a severe rinse in the tap water and then I load my grain into the tops of the bag so it is closer to the steam. Every thing mixes in after inoculation. I triple seal my bags then "spank" the bag on the clean table to ensure a good seal has been achieved.

I have a two part clean room, the second room joining the first and reserved exclusively for culture storage. I also keep blank petri sleeves and gloves, alcohol paper towels etc. This room is lined with shelves and I keep (when I am working) the shelves lined with bags and g1 jars. I find maintaining cultures in a "clean" environment really facilitates my contam rate, which is hovering somewhere around infinity.

Bags are the figggin word. I can pc more media in my 1941 modified, loading six 5lb sawdust bags in a shot. The bottom line in cultivation is the higher the spawn rate at each transfer the faster and more vigorously the myc captures the sub and that always terminates in a spectacular first flush. I think of it like slow moving myco-fireworks, the end always being worth the wait.


may the myc be with you, may it be with us all :dancer:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14174929 - 03/24/11 10:03 AM (13 years, 2 months ago)

right on.. i'm gons get me a 941 75x here in a week, this will def, help my PC'ing tasks, cause my 921 can't handle my work load.
What setting do you set your sealer on?
and do you just hold it down, till the light/heating element goes off and wait an additional 5 seconds? or do you press down in quick rapide successions? not being able to seel proper is keeping me back.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14175941 - 03/24/11 01:48 PM (13 years, 2 months ago)

I have the sealer that stametes sells. I tend to buy from FP on equipment because I trust their opinion on what is good equipment, and have yet to be disappointed. I use a 3.5 setting, and fold my bags so there is no pleat at top straight across, does my seal go, I seal and hold down for a few sec so the weld has time to cool with out being disturbed. Then I do two more usually above the first which is about two inches from the top.

Sealing is another thing that takes practice. I do an inoculation lab on six bags in under 20 min, and that includes cleaning.

Remember to never touch the bag with in 2 inches from the top, if you are going to get a contam it is (probably ) going to come from the wrong touch too close to the top.


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14177078 - 03/24/11 04:51 PM (13 years, 2 months ago)

ya trying to learn how to handle bags.. especialy since I really want to get into edibles, it's pretty much a must.
My hood messures 18x24 so I can do bags just a little akward.

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14177638 - 03/24/11 06:21 PM (13 years, 2 months ago)

Don't worry about it man. I have a 12x24 hood and I do 20 bags at a time in about 45 min.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14179349 - 03/24/11 11:15 PM (13 years, 2 months ago)

Quote:

TheShroomJew23 said:
Don't worry about it man. I have a 12x24 hood and I do 20 bags at a time in about 45 min.



nice one.:super:
So how do you prep your grains for bags? I finaly surmounted the biggest challange which was grains and got the quart thing down, but know that bags require special attention.
what size PC do you have?
I plan on getting an AA 75X soon but right now I'm using the AA921 which you can only do 7-10 quart jars. I did two bags with 12 cups of rye each for 2.5 hours at 15 psi.. Could I put more bags in my pc or Not?
was 2.5 for two bags too much?
Thanks to you as well as mycoelf for all your help.

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14179559 - 03/24/11 11:55 PM (13 years, 2 months ago)

I use a presto 22 quart pc for my bags. I can fit 6- 3 pound bags or even 4 pound bags. I use 5 gallon buckets to soak my grain, then I cook it in a large drum, then I use buckets with holes cut to drain the grain.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14179846 - 03/25/11 01:02 AM (13 years, 2 months ago)

how many cups would be equal to 3lbs?
also how long do ya pc them for?

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14185047 - 03/26/11 12:55 AM (13 years, 2 months ago)

I use a scale and that is 3 pounds wet. I pc them for an 1 hour 45 mins, let it depress totally on its own, then I wait like another 30 mins, finally I let them sit on the shelfs. The cooling creates pressure and refills my bags, they usually blow up to their full potential in a few days. They are as simple as smoking a bowl once you have your rhythm  down.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14186537 - 03/26/11 12:15 PM (13 years, 2 months ago)

so 1h45m is enough to successfuly sterilize 6-3lbs bags of RYE?:strokebeard:
Also do you seal them before you PC them?
If so it sounds like you do LC if I'm not mistaken?
If so do you add the injector port after you pc and how much lc do you use per bag?

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Invisiblemycoelf
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Re: 1st backup slants attempt. [Re: cyanara]
    #14186633 - 03/26/11 12:38 PM (13 years, 2 months ago)

the way you pack your pc has a correlated effect on pc times. Everyone has a little different take on substrate prep, so a thorough rinse after the simmer/soak phase irrigates and washes tons of bacteria/contams away, you can get indicator strips to place in your most buried location in your pc to give you an idea if your time and temp is in the zone. You could also pc some sub and set it aside as a control to find out if an unopened un inoculated bag develops any contams
:lolocaust:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14186675 - 03/26/11 12:49 PM (13 years, 2 months ago)

I soak longer than the 24 hrs normally recommended. It helps to germinate more bacteria.


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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14186681 - 03/26/11 12:51 PM (13 years, 2 months ago)

I do use LC also.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14188509 - 03/26/11 07:22 PM (13 years, 2 months ago)

ya last time I did the more than 24h soak I had a gelatin substance on the surface of the water..
I might of asked this before but what is your take on senences?
I've heard it can happen quikly and some say it might never happen as long as you keep the mycelium running?
Other than using a back up can I just purpetualy keep transfering to plates? especialy if it's an iso VS. a clone?

Edited by cyanara (03/26/11 07:33 PM)

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: cyanara]
    #14188773 - 03/26/11 08:02 PM (13 years, 2 months ago)

If you isolate from a print, just store a master and you will always have something to go back. When I do my LC, I make a quart of LC, then I store 10ml in as many vials as I will need. I use these to start my half gallon or quart batches of LC. When I run out of vials, I go back to the master slant, start another plate, and make more LC. That way by the time my mycelium hits grains, it has only touched  one dish, a master LC, and the inoculating LC. I have never experienced a problem with senescence but I also store all my plates that I use to get from print to isolate just in case I need to re-isolate. I took an oyster print and got an isolate, then I took the leading edge of growth and did 20 plates in succession with the same agar, and 20 plates changing agar recipes every four plates. The first 20 had problems by about plate 14 at colonizing the whole dish. The second batch of 20 however never showed any problems. I still have a sample from the final plate of the second batch that I use from time to time with no problems.


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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14190013 - 03/27/11 12:59 AM (13 years, 2 months ago)

nice. I really appreciate you and mycoelf taking the time to answer my question and help me out, it means the world to me. I really like to be able to keep this thread going and talking to you guys and picking your brains. If you could please PM me your proceedure in detail I would be super stoked.
I know that you guys are way more advanced than me but I would like to gift you guys with an edible of your choice..
Let me know and I will get a master slant for you guys for all the help you are giving me. You probably already have these but if I can I would like to gift you guys as a token of appreciation.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14204993 - 03/29/11 05:46 PM (13 years, 2 months ago)

Good luck buddy.... I have been doing backups on all my masters since a few years back when I was reaaly inexperienced and senesced the living hell out of all my strains.... and was basically fucked. had to start over from the get go....

Now I have backups in case I make stupid mistakes....


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: shroomie_glen]
    #14207352 - 03/30/11 12:56 AM (13 years, 2 months ago)

we will se what happens, been working hard on my genetics and managed to get two seperate iso's on seperate media at a P-3..

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Re: 1st backup slants attempt. [Re: cyanara]
    #14208634 - 03/30/11 10:11 AM (13 years, 2 months ago)

Quote:

cyanara said:
we will se what happens, been working hard on my genetics and managed to get two seperate iso's on seperate media at a P-3..





:thumbup::headbang:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14208715 - 03/30/11 10:28 AM (13 years, 2 months ago)

from the same P-2 dish..

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Re: 1st backup slants attempt. [Re: cyanara]
    #14209634 - 03/30/11 01:35 PM (13 years, 2 months ago)

here figured you guys would appreciate this.
It's my sweet potato dextros agar (SPDA), this was taken this morning 9 days after and are both P-3 values. Red Boy and Brazilian.

notice how thick the seperation is?

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Re: 1st backup slants attempt. [Re: cyanara]
    #14210262 - 03/30/11 03:53 PM (13 years, 2 months ago)

kick ass, mare sure to include these pics in the mycelium on agar thread, http://www.shroomery.org/forums/showflat.php/Number/8328896 and get the leading edge of your new isolates into slants.a.s.a.p. Remember RR's trick about putting he little piece of wood in the slant, I used toothpicks pre-soaked in a 10% ME water and PC'd with on the same run with agar.:rockon:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisiblecyanara
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Re: 1st backup slants attempt. [Re: mycoelf]
    #14210487 - 03/30/11 04:31 PM (13 years, 2 months ago)

I was gona use those stir stix from starbucks..
I'm assuming 10%ME is (malt extract)?
Do you soak the wood for 30 then just add to plain agar?
I was planning on doing it your way, water suspension, the shroomjew's way and experimenting with some agar types. like just boiling the sweet potato and adding that to the agar, no dextros or other additives. these seem to be pretty solid genes.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14210552 - 03/30/11 04:41 PM (13 years, 2 months ago)

I do both but I like using water suspension the best. I have had to re-do 150 slants because their time was up. I moved them to water, now I can stare at them and use them for around five years. Fuck now I am going to be bored, just kidding. :stoned:


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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14210674 - 03/30/11 05:03 PM (13 years, 2 months ago)

I'll probably sterilize my enoc loop and use that to carfuly scrap some mycelium into the tub of water.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14210715 - 03/30/11 05:11 PM (13 years, 2 months ago)

I take my scalpel, scrape some mycelium off the plate, JUST MYCELIUM, and put in the vial then shake it hard and store with parafilm around the cap.


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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14211420 - 03/30/11 07:14 PM (13 years, 2 months ago)

when you scrap it, do you hold the plate over the vial or grab up as much mycelium as possible and put it into the vial?
If you do number to I assume you don't have any part of the scalpel touch the interior?
Do you sterilize your scalpel along with everything else and then sanitize with alcohol and flame?

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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14211823 - 03/30/11 08:28 PM (13 years, 2 months ago)

Quote:

TheShroomJew23 said:
I take my scalpel, scrape some mycelium off the plate, JUST MYCELIUM, and put in the vial then shake it hard and store with parafilm around the cap.




Can you store all species like this?

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InvisibleTheShroomJew23
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Re: 1st backup slants attempt. [Re: Jay Sav]
    #14212266 - 03/30/11 09:54 PM (13 years, 2 months ago)

Quote:

Quote:

TheShroomJew23 said:
I take my scalpel, scrape some mycelium off the plate, JUST MYCELIUM, and put in the vial then shake it hard and store with parafilm around the cap.



Can you store all species like this?




I do not want to jump the gun and say yes, so maybe.


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Re: 1st backup slants attempt. [Re: TheShroomJew23]
    #14213874 - 03/31/11 09:06 AM (13 years, 2 months ago)

I am sorry to have been confusing. What I ment to say is that I soak the tooth picks in a malt extract solution in a small beaker and then pc on the same run, with a batch of agar. I would not run the pc just for toothpicks, I try to make my runs count as every one cost in overhead. The agar and the toothpicks are a separate media.

I usually start the week with a "tissue day" extra clean lab, pour agar, make plates, make slants, sometimes g1, then the rest of the week is cooking wood for inoculation. I like to start my week with some agar work just so I easily keep up on making cultures, and it is sometimes a half day, but usually I do agar then go cook wood for the first batch of the week.:dancer:


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Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14214161 - 03/31/11 10:34 AM (13 years, 2 months ago)

so do you add the tooth picks that have been soaking and pc'd in the ME solution to the agar later? I'm just confused with the tooth picks being separate from the agar.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14215304 - 03/31/11 02:55 PM (13 years, 2 months ago)

tooth pics alone, in a small beaker submerged in 10% ME. covered in tinfoil. Drain water on flow bench, use tweezers to transfer into inoculated slant.

The agar has nothing to do with the procedure. I was just commenting that the toothpicks Can share an autoclave session with things like agar, or lc media etc. :grin:


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Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14215603 - 03/31/11 03:54 PM (13 years, 2 months ago)

ya I understood that you where saying that it could share a PC session, but wasn't really understanding the rest.
So you transfer these to a tube of myceliated agar and they grow on the tooth picks and then you can use them to enoculate am I correct?
I also heard of a method involving paper cat litter pellets for storage?

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Re: 1st backup slants attempt. [Re: cyanara]
    #14215738 - 03/31/11 04:23 PM (13 years, 2 months ago)

exactly :bigblunt: , I inoculate my tube and place the tooth pic with one end touching the wedge and the other just in tweezers reach of just inside the neck. The agar "adheres" the toothpick in place. They are kept horizontal to grow a little, and once your myc grabs the toothpick and the agar base, they go into the cold.


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Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14215797 - 03/31/11 04:38 PM (13 years, 2 months ago)

nice I'll have to try this.
Do you use tubs?
whats your initiation strategy?

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Re: 1st backup slants attempt. [Re: cyanara]
    #14219675 - 04/01/11 09:12 AM (13 years, 2 months ago)

your hood is bigger than mine, I lay the bag horizontal and open the top resting against the sealer. I inoculate fold the bag over and seal. I have done hundreds of bags and had very little contaminant


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Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14219689 - 04/01/11 09:20 AM (13 years, 2 months ago)

thanks... got everything ready to do my slants today, now I just got to get these damn tubs to fruit... been 3 days and no knotts yet. the rhizo's are completely vertical and so thick I could cut them?
Hope I get some mushies soon, i'm getting anxious. want to eat some

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Re: 1st backup slants attempt. [Re: cyanara]
    #14220689 - 04/01/11 12:46 PM (13 years, 2 months ago)

I figured since i just did some, and its relevant, ill post mine in this thread!
These are not the first i've done...
Did some in glass vacutainers:rolleyes:, but i dont consider them, as they have never been used past a trial...

Pictured below are the slants...Sorry for the horrible quality, my macro mode sucks...


Some information on the tubes...
I received some cheap pre-sterilized PP tubes and caps from cynmar.  They were advertised as all PP, but turns out the cap wasnt and melts during autoclaving... :shrug:
Anyways, to make use of a product other wise laying around in my lab, i sterilized some agar and filled them infront of the hood with a sterilized syringe...
Cooled them at a slant and then inoculated them with a few iso's around the house....
Wasnt hoping for great results, but got some pretty decent ones...:awesomenod:

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Re: 1st backup slants attempt. [Re: total]
    #14221136 - 04/01/11 01:58 PM (13 years, 2 months ago)

nice, thank you for your contribution.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14222323 - 04/01/11 05:41 PM (13 years, 2 months ago)

my pinning is strategy is OH :poop:  I left these in the bag too long. I have a clean room full of bags. I rack them like wine. I put them up and then forget about them, and then put them out or apply them when and where they will do the most good.

I maily produce spawn for other people. Most of my cultivation efforts are along the same lines. Make a bunch of spawn, put out a patch somewhere and forget about them until they show back up.


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Re: 1st backup slants attempt. [Re: mycoelf]
    #14222387 - 04/01/11 05:54 PM (13 years, 2 months ago)

Quote:

mycoelf said:
I maily produce spawn for other people.




My friend used to buy pre-colonized mycobags off this dude by the truckload for $50 a bag :rolleyes:

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Re: 1st backup slants attempt. [Re: total]
    #14225104 - 04/02/11 08:53 AM (13 years, 1 month ago)

dude thats a pretty fucking sweet racket.

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Re: 1st backup slants attempt. [Re: cyanara]
    #14225153 - 04/02/11 09:13 AM (13 years, 1 month ago)

Yup...
This was years ago, and he just fruited them straight out the bag...
If only he knew about spawning to bulk back then...:super:

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