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Shop: North Spore Bulk Substrate, Injection Grain Bag   Mushroom-Hut Liquid Cultures, Substrate Bags   Original Sensible Seeds High THC Strains

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OfflineSUMYUNGGUY
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Registered: 12/29/10
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Last seen: 12 years, 10 months
STOKEDD!
    #14119221 - 03/14/11 12:43 PM (12 years, 10 months ago)

My syringes came in today... i'll be innoculating 20 quart jars of organic rye berries. I have two questions... is an hour and a half long enough to pc at 15psi andd is it really necessary to wipe the needle with an alcohol pad after I flame sterilized it? Thankss


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InvisibleLuger0815
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Registered: 12/21/10
Posts: 1,677
Loc: @ Home
Re: STOKEDD! [Re: SUMYUNGGUY]
    #14119233 - 03/14/11 12:45 PM (12 years, 10 months ago)

90 minutes@15 psi is enough.
Dont wipe with alcohol after flame sterilize.


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OfflineAjaxx
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Registered: 03/06/11
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Re: STOKEDD! [Re: Luger0815]
    #14119264 - 03/14/11 12:50 PM (12 years, 10 months ago)

what strain are you workin with?

and also, hopefully you know this by now. wait for 24-48 hours after PCing to inoculate jars.


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Offlinecuriousgeorge91
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Registered: 02/10/11
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Re: STOKEDD! [Re: Ajaxx]
    #14119284 - 03/14/11 12:53 PM (12 years, 10 months ago)

dont wipe it after flame sterilize, do it before (right after inoc of jar) before flaming and doing the next.


--------------------
The shoe that fits one person pinches another; there is no recipe for living that suits all cases.

-  Carl Jung


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OfflineAjaxx
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Re: STOKEDD! [Re: curiousgeorge91]
    #14119307 - 03/14/11 12:57 PM (12 years, 10 months ago)

do you wipe the needle and flame sterilize before every jar?


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OfflineSUMYUNGGUY
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Registered: 12/29/10
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Re: STOKEDD! [Re: Ajaxx]
    #14119356 - 03/14/11 01:08 PM (12 years, 10 months ago)

ohhh... okay, just watched a rr video, you wipe with alcohol between jars to prevent cross contamination. got it! Yo ajaxx... gotta love the nectar brah! i'll be seeing him at starscape this year!


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OfflinePsilocyentist
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Registered: 11/04/10
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Re: STOKEDD! [Re: SUMYUNGGUY]
    #14119456 - 03/14/11 01:33 PM (12 years, 10 months ago)

Quote:

SUMYUNGGUY said:
ohhh... okay, just watched a rr video, you wipe with alcohol between jars to prevent cross contamination. got it! Yo ajaxx... gotta love the nectar brah! i'll be seeing him at starscape this year!



No you flame in between jars to prevent cross contamination. Alcohol wiping isn't necessary, you can do it to get the chunks of verm/substrate off after, but the flaming is whats doing the sterilizing. If you flame then wipe with alcohol, your needle is no longer sterile. :shrug:


--------------------
I know, I know you probably scream and cry

That your little world won't let you go



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OfflineAjaxx
Amateur Mycologist
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Registered: 03/06/11
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Re: STOKEDD! [Re: SUMYUNGGUY]
    #14119748 - 03/14/11 02:43 PM (12 years, 10 months ago)

Quote:

SUMYUNGGUY said:
ohhh... okay, just watched a rr video, you wipe with alcohol between jars to prevent cross contamination. got it! Yo ajaxx... gotta love the nectar brah! i'll be seeing him at starscape this year!




im seein him next month for my 5th time. then ill see him at wakarusa again :awecid:


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Offlinecuriousgeorge91
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Re: STOKEDD! [Re: Ajaxx]
    #14119805 - 03/14/11 02:57 PM (12 years, 10 months ago)

Quote:

Ajaxx said:
do you wipe the needle and flame sterilize before every jar?




yup.
no need to do it on every hole because if its contammed its contammed, but you wanna do it between each one to preven cross contamination.


--------------------
The shoe that fits one person pinches another; there is no recipe for living that suits all cases.

-  Carl Jung


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OfflineSUMYUNGGUY
Stranger

Registered: 12/29/10
Posts: 5
Last seen: 12 years, 10 months
Re: STOKEDD! [Re: curiousgeorge91]
    #14126946 - 03/15/11 07:56 PM (12 years, 10 months ago)

yahhh... its all about the womp womps!


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