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OfflinePerun
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Registered: 11/11/10
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14113638 - 03/13/11 10:54 AM (12 years, 11 months ago)

If they r REusable petris then yeah,u PC them...if not...u dont REuse them! :laugh:


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First:                                                        Then:

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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: Perun]
    #14114142 - 03/13/11 12:56 PM (12 years, 11 months ago)

crap they r disposable.. guess i cant PC them ..
u sure 99% alcohol wont do the trick?:laugh:


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Offline3n1gm4
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Registered: 01/13/11
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14114271 - 03/13/11 01:34 PM (12 years, 11 months ago)

Maybe this will help you decide, some bacteria thrive at boiling temps in volcanic sulfur springs with chemicals that are deadly to almost any living organism.

Buy some autoclaveable petris and you can just PC them to make sure.

You could try alcohol and it might work but why go to all that trouble for maybe nothing.


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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InvisibleDr.Boomstik
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Registered: 10/11/10
Posts: 128
Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14114513 - 03/13/11 02:39 PM (12 years, 11 months ago)

Quote:

btw is it possible to REuse petri dishes if i clean them with 99% alcohol or somethin?




If they're glass you could def. PC them but if they're plastic I believe the normal procedure is to just throw them away because they're so cheap. However, if using antibiotic agar I see no reason why you couldn't give them a good wipedown with 70-80% alcohol* under sterile conditions, pour piping hot agar in and hope for the best...I could be wrong but it's worth a try, the worst that will happen is germination of mold spores which could be isolated away from. best of Luck!

* I too used to believe that 99% alcohol was the best for cleaning but it's just not true...the water is added to the alcohool for a reason, to allow germ cells to accept the alcohol into the cell wall where it kills by evaporation (search it for more accurate explanation but you get the point). The cells are "intelligent" enough to know somethin' is up but by adding water you can 'trick' them into accepting it and thus killing them. So if you have that alcohol mix it w/ water or 70% alcohol to dilute it a bit and make it a much more effective sanitizer.


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Expect poison from standing water.

The best wine is the oldest. The best water, the newest.

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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: Dr.Boomstik]
    #14114540 - 03/13/11 02:48 PM (12 years, 11 months ago)

70% also evaporates slower giving it more time to disinfect...


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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Offlinechevpor
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Registered: 02/20/11
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14118508 - 03/14/11 09:04 AM (12 years, 11 months ago)

Nice to learn about alcohol. Always thought that the 99% was the best

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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: chevpor]
    #14119588 - 03/14/11 02:04 PM (12 years, 11 months ago)

yeah, i learned something new as well. tnx guys :laugh:


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: k00laid]
    #14127774 - 03/15/11 10:21 PM (12 years, 11 months ago)

Hmm.  I could be incorrect about this, but I was always under the impression that P. cubensis was a phototropic species.


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In the end, my friend, we will all be together again.

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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14127802 - 03/15/11 10:27 PM (12 years, 11 months ago)

A wise man once told me that they grow towards the fresh air supply, and I have seen many pictures where they do just that.

He also said that they get their energy from the light somehow, not telling why but just that they did it somehow.



Maybe it is storing energy to grow in the dark.


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14127973 - 03/15/11 10:54 PM (12 years, 11 months ago)

Yeah, the tendency to grow toward fresh air supplies is known as anemotropism.  There are various other known tropisms (stimuli that cause directional growth). 

If anyone is interested to learn more about the tropisms, here are the ones that I can remember off the top of my head:
1. Chemotropism (introduction/presence of specific chemicals)
2. Anemotropism (mentioned above)
3. Positive and/or Negative Gravitropism (I believe that some species of fungus have mycelia that is driven by negative gravitropism whereas the stipe/cap is effected by positive gravitropism...not 100% on that though)
4. Thigmotropism (physical contact with a...usually...external object)
5. Phototropism (light stimulus)
6. Galvanotropism (this one I know the least about, I know that it deals with electrical potentials and is generally seen more often in the realm of neuroscience, although I believe that the action overlaps into mycological physiology, as well as others)

I know that there are more, but I'm sure a simple google search would provide you with plenty of information on these categories as well as the remaining tropisms that are not listed here.

I am going to see if I can pull some information off of my archives about the physiology of P. cubensis.  If I run into anything that seems to be valid information, I will post it here.


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In the end, my friend, we will all be together again.

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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14128765 - 03/16/11 01:39 AM (12 years, 11 months ago)

If I blindfold myself and hit you with a stick will candy fall out of you?
Or would you just take the stick from me?
LOL locked in a skin pinata...


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14130059 - 03/16/11 10:53 AM (12 years, 11 months ago)

Yup, but the candy usually falls out of my pockets and it's not the kind that you give to your kids, and the only sticks I take from people come from Thailand.  Hehehe.


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In the end, my friend, we will all be together again.

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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14165358 - 03/22/11 03:46 PM (12 years, 11 months ago)

I ran into a source that lists Psilocybe cubensis as anemotropic, phototropic and gravitropic.  I believe it's mentioned in the cultivation book by Ogame and Nicholas.


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In the end, my friend, we will all be together again.

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Offlinebilbobaggins
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Registered: 07/11/10
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Last seen: 12 years, 11 days
Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14549588 - 06/02/11 06:20 AM (12 years, 8 months ago)

Could anyone please help with my problem, if Jars that have been too hot on the bottom half, causing mycelium to disappear leaving wet grains will recover? The top half of all the jars look fine, due to heat from the jars and first time with so many jars the temperature was 2 or 3 degrees Celsius different from the top of the incubator to the bottom. With lowering the temperature and leaving alone will they recover fully?

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