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Offlinejenia1
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Yet another Incubator Heating problem
    #14108917 - 03/12/11 12:59 PM (12 years, 10 months ago)

hey there guys. after lots of delays ive finally completed my incubator
and on the 1st try the light bulb which is used for heating has melt down the buttom of the fridge and went kaBooM lol.
so now im using another bulb this time on a stone surface
the main reason why this this is happened  is because the bulb is located at the buttom and the heat sensor is at the top so the bulb reaches so called"critical temps" like the nuclear plant in japan <= lame jk.
i was wondering how do ppl compare the temps anywhere at the incubator?
lookin for some good advice tnx for help.


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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14108931 - 03/12/11 01:04 PM (12 years, 10 months ago)

what are you incubating..eggs:laugh2:


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: penhed]
    #14108944 - 03/12/11 01:07 PM (12 years, 10 months ago)

Quote:

penhed said:
what are you incubating..eggs:laugh2:




petri dishes atm, and spawn jars abit later(hopefully):cool:


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Invisiblepenhed
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14108963 - 03/12/11 01:12 PM (12 years, 10 months ago)

nice...no harm intended ,,be  careful with possible fire hazards:smile:


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InvisibleDr.Boomstik
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14108985 - 03/12/11 01:19 PM (12 years, 10 months ago)

I'd say that you can stop worrying about the incubator completely and trash it brotherman. No matter how well you regulate the temperature it's still going to be more hassle and cause you more failure than needed. Normal room temp. is all that's needed and is preferred by healthy mycelium. They're exothermic in nature, meaning they generate heat as they go so if you can stand your house in a t-shirt they'll be more than fine. Also, bottling them up in a stale air environment will encourage bacterial contamination to flourish. Yeah, I know, even if you sterilize your rye or use brf which doesn't (generally) harbor much bacterial endospores you still have a 2 week window and it will slow them down considerably. Remember sometimes less is more - so just put them on a shelf in your room with regular ambient airflow and enjoy.

If you insist on an incubator atleast put the heat source above the jars because you don't want that heat rising into your jars - esp. when it's 10-15 degrees warmer in them than outside temps anyway. Just tryin' to help you out my friend. learn from the failures of others (been there :frown: ) and don't forget the search engine. ~namaste~


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Expect poison from standing water.

The best wine is the oldest. The best water, the newest.


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: penhed]
    #14108992 - 03/12/11 01:20 PM (12 years, 10 months ago)

Quote:

penhed said:
nice...no harm intended ,,be  careful with possible fire hazards:)



yeah.. thats wat im afraid of.. im barely at home and this seems to be more dangerous then i thought


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: Dr.Boomstik]
    #14109022 - 03/12/11 01:27 PM (12 years, 10 months ago)

Quote:

Dr.Boomstik said:
I'd say that you can stop worrying about the incubator completely and trash it brotherman. No matter how well you regulate the temperature it's still going to be more hassle and cause you more failure than needed. Normal room temp. is all that's needed and is preferred by healthy mycelium. They're exothermic in nature, meaning they generate heat as they go so if you can stand your house in a t-shirt they'll be more than fine. Also, bottling them up in a stale air environment will encourage bacterial contamination to flourish. Yeah, I know, even if you sterilize your rye or use brf which doesn't (generally) harbor much bacterial endospores you still have a 2 week window and it will slow them down considerably. Remember sometimes less is more - so just put them on a shelf in your room with regular ambient airflow and enjoy.

If you insist on an incubator atleast put the heat source above the jars because you don't want that heat rising into your jars - esp. when it's 10-15 degrees warmer in them than outside temps anyway. Just tryin' to help you out my friend. learn from the failures of others (been there :frown: ) and don't forget the search engine. ~namaste~




frankly i put too much effort on the incubator and it will suck to see that work go down the drain.
but the main issue here is that my "normal room temp" isnt that normal
its around 10 celcius now and up to 44  in the summer picks..
so i think i have no choise but to use incubator


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InvisibleDr.Boomstik
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109096 - 03/12/11 01:42 PM (12 years, 10 months ago)

Quote:

frankly i put too much effort on the incubator and it will suck to see that work go down the drain.





^ My point exactly. So why would you want to continue the effort and have your success hampered over your pride? You're not the only one who put a lot of work in to find out it was a waste of time. It's all gravy - we've all done it but if we wished to succeed we changed our ways, quick. I know it sucks but you could use it to raise chickens :smile: if it's that beautiful. Not tryin' to be a dick but learn from those who have failed and let not they're work go in vain. just sayin.


10 degrees celsius isn't too far off - 15-20 degrees C will be fine so just get a space heater and heat the surrounding room to desired temps. They'll still grow at 10 degrees C just a lil slower. My first grows were in a room that never got over 50 degrees F.


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Expect poison from standing water.

The best wine is the oldest. The best water, the newest.


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: Dr.Boomstik]
    #14109097 - 03/12/11 01:42 PM (12 years, 10 months ago)

The process of colonization is indeed exothermic, but the change in temperature is negligible, a few degrees.  Colonization time is greatly decreased by utilizing an incubator (unless, as stated, the ambient temperature of your house is 82-86 degrees Fahrenheit).  I will generally set my incubator's submersible heater at 82 to factor in the exothermic reaction.

I have never once experienced a contaminant that was the result of using an incubator.  If you are using filtered jars, you are working with a closed, sterile system that would be VERY difficult to contaminate.  Rye, in my experience, is FULL of bacterial endospores that should be germinated before you PC your jars.  Also, contamination CAN occur much faster than two weeks.

I would highly suggest using a double-tub incubator as you can evenly distribute the temperature and maintain it at whatever level you choose (assuming, of course, that you have an adjustable, fully submersible heater).  This model runs the risk of combining electricity and water, but as long as you get a FULLY submersible fish-tank heater you shouldn't have any problems.

I'm sorry, I don't mean to come off as argumentative or rude, I am drawing from personal experience and a fair amount of research.  Don't misunderstand, I am certainly not implying that you aren't, but luckily, this is a fairly forgiving science and there are many routes to the same end.


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In the end, my friend, we will all be together again.


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14109326 - 03/12/11 02:32 PM (12 years, 10 months ago)

TIME IS OF AN ESSENCE my friends, and it would be awefull to waste it
i heared a drop of 10 degrees F slows down the colonization by 50%
thats why i want conditions to be idial.
p.s i burned another lamp after covering it with thin foil lolz,cuz i heared mycilium doesnt like light too much.
an idea just came to me, i think to add a small fan to destribute the heat faster and save the lamp from overheating, cross ur fingers:D


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Offlinek00laid
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109332 - 03/12/11 02:34 PM (12 years, 10 months ago)

you dont need an incubator.

mycelium likes light just fine.


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109366 - 03/12/11 02:44 PM (12 years, 10 months ago)

You don't want to drop the temperature while the mycelia is colonizing.  You drop the temperature AFTER it is done colonizing and is ready to be put into fruiting chambers.  A drop in temperature isn't actually essential with P. cubensis (certain species require a drop in temperature to set forth primordia), but I still do it, because, well, it works for me.

Yeah, as mentioned, mycelia likes light just fine.  It actually needs a certain amount of light to, essentially, know which way is "up". 

Using a fan should help to prevent overheating.  What kind of bulbs are you using?  They have bulbs that are designed (basically) for this purpose.  Heat lamps. 

As mentioned, an incubator is not an essential item of cubensis cultivation, but again, I use one because it works well for me and is a bit more incognito.

Good luck.


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Offlinek00laid
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14109405 - 03/12/11 02:57 PM (12 years, 10 months ago)

Quote:

DiMiTriSouljah said:
It actually needs a certain amount of light to, essentially, know which way is "up".




then why do mushrooms grow in total darkness up?


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14109411 - 03/12/11 02:58 PM (12 years, 10 months ago)

im using simplest lamps on the market
1st one was 100W and it caused the fridge buttom to melt before it exploded lol.. and the last lamp i sent to the grave was around 60W


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Offlinek00laid
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109419 - 03/12/11 03:00 PM (12 years, 10 months ago)

why are you incubating >.>


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: k00laid]
    #14109430 - 03/12/11 03:02 PM (12 years, 10 months ago)

Quote:

k00laid said:
why are you incubating >.>




im incubating the agar cultures to see if theres any contams be4 inoculating


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Offlinek00laid
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109444 - 03/12/11 03:05 PM (12 years, 10 months ago)

you dont need to incubate your agar cultures to check them for contams.

you can do that in room temperature


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: k00laid]
    #14109451 - 03/12/11 03:07 PM (12 years, 10 months ago)

Quote:

k00laid said:
you dont need to incubate your agar cultures to check them for contams.

you can do that in room temperature




wont it take longer?


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Offlinek00laid
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14109588 - 03/12/11 03:38 PM (12 years, 10 months ago)

to check for contams?

no


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: k00laid]
    #14113489 - 03/13/11 10:10 AM (12 years, 10 months ago)

btw is it possible to REuse petri dishes if i clean them with 99% alcohol or somethin?


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OfflinePerun
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14113638 - 03/13/11 10:54 AM (12 years, 10 months ago)

If they r REusable petris then yeah,u PC them...if not...u dont REuse them! :laugh:


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First:                                                        Then:


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: Perun]
    #14114142 - 03/13/11 12:56 PM (12 years, 10 months ago)

crap they r disposable.. guess i cant PC them ..
u sure 99% alcohol wont do the trick?:laugh:


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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: jenia1]
    #14114271 - 03/13/11 01:34 PM (12 years, 10 months ago)

Maybe this will help you decide, some bacteria thrive at boiling temps in volcanic sulfur springs with chemicals that are deadly to almost any living organism.

Buy some autoclaveable petris and you can just PC them to make sure.

You could try alcohol and it might work but why go to all that trouble for maybe nothing.


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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InvisibleDr.Boomstik
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14114513 - 03/13/11 02:39 PM (12 years, 10 months ago)

Quote:

btw is it possible to REuse petri dishes if i clean them with 99% alcohol or somethin?




If they're glass you could def. PC them but if they're plastic I believe the normal procedure is to just throw them away because they're so cheap. However, if using antibiotic agar I see no reason why you couldn't give them a good wipedown with 70-80% alcohol* under sterile conditions, pour piping hot agar in and hope for the best...I could be wrong but it's worth a try, the worst that will happen is germination of mold spores which could be isolated away from. best of Luck!

* I too used to believe that 99% alcohol was the best for cleaning but it's just not true...the water is added to the alcohool for a reason, to allow germ cells to accept the alcohol into the cell wall where it kills by evaporation (search it for more accurate explanation but you get the point). The cells are "intelligent" enough to know somethin' is up but by adding water you can 'trick' them into accepting it and thus killing them. So if you have that alcohol mix it w/ water or 70% alcohol to dilute it a bit and make it a much more effective sanitizer.


--------------------
Expect poison from standing water.

The best wine is the oldest. The best water, the newest.


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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: Dr.Boomstik]
    #14114540 - 03/13/11 02:48 PM (12 years, 10 months ago)

70% also evaporates slower giving it more time to disinfect...


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14118508 - 03/14/11 09:04 AM (12 years, 10 months ago)

Nice to learn about alcohol. Always thought that the 99% was the best


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Offlinejenia1
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Re: Yet another Incubator Heating problem [Re: chevpor]
    #14119588 - 03/14/11 02:04 PM (12 years, 10 months ago)

yeah, i learned something new as well. tnx guys :laugh:


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: k00laid]
    #14127774 - 03/15/11 10:21 PM (12 years, 10 months ago)

Hmm.  I could be incorrect about this, but I was always under the impression that P. cubensis was a phototropic species.


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In the end, my friend, we will all be together again.


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Offline3n1gm4
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14127802 - 03/15/11 10:27 PM (12 years, 10 months ago)

A wise man once told me that they grow towards the fresh air supply, and I have seen many pictures where they do just that.

He also said that they get their energy from the light somehow, not telling why but just that they did it somehow.



Maybe it is storing energy to grow in the dark.


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14127973 - 03/15/11 10:54 PM (12 years, 10 months ago)

Yeah, the tendency to grow toward fresh air supplies is known as anemotropism.  There are various other known tropisms (stimuli that cause directional growth). 

If anyone is interested to learn more about the tropisms, here are the ones that I can remember off the top of my head:
1. Chemotropism (introduction/presence of specific chemicals)
2. Anemotropism (mentioned above)
3. Positive and/or Negative Gravitropism (I believe that some species of fungus have mycelia that is driven by negative gravitropism whereas the stipe/cap is effected by positive gravitropism...not 100% on that though)
4. Thigmotropism (physical contact with a...usually...external object)
5. Phototropism (light stimulus)
6. Galvanotropism (this one I know the least about, I know that it deals with electrical potentials and is generally seen more often in the realm of neuroscience, although I believe that the action overlaps into mycological physiology, as well as others)

I know that there are more, but I'm sure a simple google search would provide you with plenty of information on these categories as well as the remaining tropisms that are not listed here.

I am going to see if I can pull some information off of my archives about the physiology of P. cubensis.  If I run into anything that seems to be valid information, I will post it here.


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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14128765 - 03/16/11 01:39 AM (12 years, 10 months ago)

If I blindfold myself and hit you with a stick will candy fall out of you?
Or would you just take the stick from me?
LOL locked in a skin pinata...


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: 3n1gm4]
    #14130059 - 03/16/11 10:53 AM (12 years, 10 months ago)

Yup, but the candy usually falls out of my pockets and it's not the kind that you give to your kids, and the only sticks I take from people come from Thailand.  Hehehe.


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In the end, my friend, we will all be together again.


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OfflineDiMiTriSouljah
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14165358 - 03/22/11 03:46 PM (12 years, 10 months ago)

I ran into a source that lists Psilocybe cubensis as anemotropic, phototropic and gravitropic.  I believe it's mentioned in the cultivation book by Ogame and Nicholas.


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In the end, my friend, we will all be together again.


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Offlinebilbobaggins
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Re: Yet another Incubator Heating problem [Re: DiMiTriSouljah]
    #14549588 - 06/02/11 06:20 AM (12 years, 7 months ago)

Could anyone please help with my problem, if Jars that have been too hot on the bottom half, causing mycelium to disappear leaving wet grains will recover? The top half of all the jars look fine, due to heat from the jars and first time with so many jars the temperature was 2 or 3 degrees Celsius different from the top of the incubator to the bottom. With lowering the temperature and leaving alone will they recover fully?


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