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Invisiblecyanara
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Registered: 12/22/09
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subtrate formulation and isolation
    #14103289 - 03/11/11 10:30 AM (13 years, 2 months ago)

is it better to keep your agar substrate the same to keep the mycelium running durring isolation and once completed then switch substrate types to develope other digestive enzymes?

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14158407 - 03/21/11 09:08 AM (13 years, 2 months ago)

I read a post where RR claimed he has never changed from using MEA for his cultures.

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Invisiblebiologys
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14158642 - 03/21/11 10:23 AM (13 years, 2 months ago)

rr also purchases pre made agar, it saves him time with his busy scedule..
\
http://www.shroomery.org/forums/showflat.php/Number/6253155#6253155

and another quote from RR

Quote:

You don't need to vary the agar recipe. I've used MEA for 20+ years on all my cultures, and they're fine. Eating the same food doesn't cause senescence, cell divisions do.

When working with wild prints, I'll often use antibiotic agar, and mix it with less malt. If you buy pre-mixed agar powder from fp or sporeworks, you can mix it a bit weaker than recommended. This will keep from feeding the mold and bacteria quite so much.

When you get the video back, watch the section on strain isolation. It demonstrates the best places to take transfers.
RR



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OfflineRogerRabbitM
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Re: subtrate formulation and isolation [Re: biologys]
    #14158932 - 03/21/11 11:49 AM (13 years, 2 months ago)

I've just not found evidence of the 'digestive enzyme' training phenomena.  Honestly, if I had I'd have switched agar formulas around or switched away from rye berries, but it just hasn't been a problem, at least with the species I grow.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: RogerRabbit]
    #14159036 - 03/21/11 12:21 PM (13 years, 2 months ago)

thanks RR.. I have noticed that certain of my strains perform better on MEA while others better on PDYA.
I bought some lab grade crap from a local supply store and it contains the following per liter,

15g agar
20g malt
20g dextros
6g pepton

way too much, noticed that it made the mycelium very lazy and seems to promote mutation factors..
I just made a batch of sweet potato agar and am hoping that the complex carbs will promote some good growth.

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Offlinesolumvita
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Re: subtrate formulation and isolation [Re: cyanara]
    #14159338 - 03/21/11 01:36 PM (13 years, 2 months ago)

it does depend on the species as well, I have not been able to keep an Agaricus spp on PDA for more than 4 transfers and it starts to show sectors and lose vigour.

But oysters do not seem to be bothered.


--------------------
One of these days all the answers will be revealed until then we learn from each other!

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: solumvita]
    #14160290 - 03/21/11 04:49 PM (13 years, 2 months ago)

ya oyster seems to like the PDA better but then the my cubies seem to like the MEA slightly better as long as the sugar content is lower.
Thats why I'm hoping that the cultures will responde well to the sweet potato agar that I made 2 days ago.

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14168916 - 03/23/11 09:33 AM (13 years, 2 months ago)

Sweet potato agar eh? Let us know how that goes. Im interested.

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14169396 - 03/23/11 11:14 AM (13 years, 2 months ago)

ok so two things I've noted. 1) so far the sweet potato agar is already showing signs of thick rhizomorphic activity.. the mycelium is gearing to explode.
2) what I've noticed with nutritionaly dense mediums growth is very slow and seems to promote mutation, or more so accentuate the different strains on the plate without allowing the mycelium to grow over a large area of the plate in a short time which would in theory keep the cell divisions down to a minimum.
So basicaly say my plate grew less than the size of a us quarter and showed def sign of separation, i would be able to pick and isolate my mycelium without it ever expanding significantly.. seems that alot of time when agar isn't as dense the mycelium tends to outrun the plate befor you are able to pick up on it's true nature, because its so franticaly searching for nutrients.. def something worth looking into, but anyway as of right now the sweet potato agar is rocking and showing alot of promise.

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14174935 - 03/24/11 10:05 AM (13 years, 2 months ago)

Thats what's up, you got some pics?

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14174951 - 03/24/11 10:09 AM (13 years, 2 months ago)

ya, i'll go take some and post em. hang ten

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: cyanara]
    #14180277 - 03/25/11 03:14 AM (13 years, 2 months ago)

here are a couple pics of the new growth on my SPDA.

also I noticed that the P.Cyanofriscosa is responding extremly well to it.

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14186230 - 03/26/11 10:59 AM (13 years, 2 months ago)

Seems like the mycelium likes it enough. Its cool to see all the different variations in agar recipes that people come up with. Cool stuff

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14186556 - 03/26/11 12:20 PM (13 years, 2 months ago)

growth is average but remains extremly rhizo.
here are some new pics.

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14192242 - 03/27/11 01:14 PM (13 years, 2 months ago)

Yea, that's some healthy looking myc. Might be fun to try that with a few other types of roots/tubers... taro, yucca are a couple I can think of atm. Would be a fun little project

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14203677 - 03/29/11 01:08 PM (13 years, 2 months ago)

for sure, def worth the investigation. I'll post some more pics laterz as the growth now is very indicative of the sweet potato's massive potential.

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: cyanara]
    #14209601 - 03/30/11 01:28 PM (13 years, 2 months ago)

here you go my Red Boy and Brazillian 9 days later at a P-3 value.
Enjoy.

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Offlineesquaredx
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Re: subtrate formulation and isolation [Re: cyanara]
    #14210575 - 03/30/11 04:45 PM (13 years, 2 months ago)

Dayum! :awethumb:


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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: esquaredx]
    #14210661 - 03/30/11 05:01 PM (13 years, 2 months ago)

ya right? really thick separation in those rhizo's.. stuff is awesome. I have some other tricks up my sleeves for agar..

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OfflineJay Sav
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Re: subtrate formulation and isolation [Re: cyanara]
    #14213889 - 03/31/11 09:11 AM (13 years, 2 months ago)

That shiz is off the hook. Great pics man, that myc looks super rhizo

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: Jay Sav]
    #14214149 - 03/31/11 10:31 AM (13 years, 2 months ago)

you should give it a try. super easy to make.

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Invisiblearis
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Re: subtrate formulation and isolation [Re: cyanara]
    #14225108 - 04/02/11 08:54 AM (13 years, 1 month ago)

Could you post your sweet potato agar recipe or a link to one.

:confused:

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: aris]
    #14225172 - 04/02/11 09:18 AM (13 years, 1 month ago)

hi aris, I will make a write up and post my SPDA in the next hour or so as I will be doing that and making backups today.
Thank you for your intereste in my agar, stay tuned on this thread as I will be doing other cool agar stuff.
In the mean time here is the same P-3 isolate only on lab grad agar(MEA).

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Offlinetotal
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Re: subtrate formulation and isolation [Re: cyanara]
    #14225181 - 04/02/11 09:20 AM (13 years, 1 month ago)

Total different look there eh?
I can tell a difference between my pda and mea :super:

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: total]
    #14225201 - 04/02/11 09:28 AM (13 years, 1 month ago)

well the MEA was lab grade I bought from a supplier. the other is not PDA but SPDA (sweet potato agar) and that stuff rocks far more than anything else that I've tried so far..
Also it seems that slower growing cubensis and/or wood lovers like it better, as they digest slower. A quick variety like brazilians can perform better but seem to like the lab MEA because of its high sugar content, the nutrients are available to this explosive mycelial type.
This is just what I've observed in culture.
Also the mycelium usualy goes thru a resting phase as it colonizes a plate and during this time before it starts to grow again is when you see sectoring and/or when the mycelium will change in it;s apperance.. cultures that thrived in the SPDA where completely consistant all the way.

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Offlineshroomie_glen
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Re: subtrate formulation and isolation [Re: cyanara]
    #14226075 - 04/02/11 01:25 PM (13 years, 1 month ago)

Quote:

cyanara said:
here you go my Red Boy and Brazillian 9 days later at a P-3 value.
Enjoy.





:clap:
effing a man.... effing a (to quote lawrence)


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

Edited by shroomie_glen (04/02/11 01:25 PM)

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Invisiblecyanara
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Re: subtrate formulation and isolation [Re: shroomie_glen]
    #14227793 - 04/02/11 07:11 PM (13 years, 1 month ago)

:biggrin:
thanks man

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Invisiblefngbronco
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Re: subtrate formulation and isolation [Re: cyanara]
    #14227872 - 04/02/11 07:26 PM (13 years, 1 month ago)

That's awesome! Can't wait for the tek!


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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