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cybernoodle2000
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Registered: 02/11/11
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Last seen: 12 years, 5 months
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LC, Is this Panaeolus cinctulus mycelium?
#14100576 - 03/10/11 08:49 PM (13 years, 10 months ago) |
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Three weeks ago, a pint jar was prepared with honey and spores from a wild Panaeolus cinctulus print. Jar has been in the dark, 70-80F and only stirred a couple of times during the three weeks. So does this look like Panaeolus cinctulus mycelium?
Various stages of agitation.





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Doc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100592 - 03/10/11 08:51 PM (13 years, 10 months ago) |
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LC from a wild print is unlikely to work. You'll need to shoot a test jar to know anything. Nice pics though.
-------------------- You make it all possible. Doesn't it feel good?
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Stains Blue
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Registered: 01/17/11
Posts: 963
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100627 - 03/10/11 08:56 PM (13 years, 10 months ago) |
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If you put spores from a wild print straight into a LC with out isolating the mycelium from contamination I your HIGHLY likely to have contamination.
But here is a pic on Pan cyan Viets. To compare with.

But this looks contaminated.
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cybernoodle2000
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Doc_T]
#14100679 - 03/10/11 09:02 PM (13 years, 10 months ago) |
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Quote:
Doc_T said: LC from a wild print is unlikely to work. You'll need to shoot a test jar to know anything. Nice pics though.
Ya It is likely to produce con-tams from a wild print I know. But does what is growing look like Pan. Cin. mycelium?
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Microppose
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Stains Blue]
#14100685 - 03/10/11 09:03 PM (13 years, 10 months ago) |
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It's impossible to tell what species of mushroom just by the mycelium alone. Especially in an LC.
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cybernoodle2000
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Registered: 02/11/11
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Stains Blue]
#14100694 - 03/10/11 09:05 PM (13 years, 10 months ago) |
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Quote:
Stains Blue said: If you put spores from a wild print straight into a LC with out isolating the mycelium from contamination I your HIGHLY likely to have contamination.
But here is a pic on Pan cyan Viets. To compare with.

But this looks contaminated.

I noticed mine cleared up a lot compared to yours. What about it looks like cantams?
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cybernoodle2000
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Microppose]
#14100713 - 03/10/11 09:07 PM (13 years, 10 months ago) |
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Quote:
Azurascender said: It's impossible to tell what species of mushroom just by the mycelium alone. Especially in an LC.
The print was taken from a wild collected specimen and identified as Pan. Cin. on this site. I am just trying to figure out if what we see is mycelium..
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Stains Blue
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Registered: 01/17/11
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100743 - 03/10/11 09:12 PM (13 years, 10 months ago) |
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You have to inoculate a test jar to determine weather or not it has contamination. But that cloudy mess you have there looks contaminated to me. To be 100% certain shoot up a jar.
with my experiance with LC
cloudy to clear = a good sign & clear to cloudy = contamination.
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cybernoodle2000
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Stains Blue]
#14100773 - 03/10/11 09:16 PM (13 years, 10 months ago) |
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Quote:
Stains Blue said: You have to inoculate a test jar to determine weather or not it has contamination. But that cloudy mess you have there looks contaminated to me. To be 100% certain shoot up a jar.
with my experiance with LC
cloudy to clear = a good sign & clear to cloudy = contamination.
I'm not trying to find out if it is contaminated lol. I just want to know if whats growing on the bottom of the jar looks like pan mycelium. What ever it is, ther is alot of it, ofcourse it is going to make the solution cloudy.
Edited by cybernoodle2000 (03/10/11 09:20 PM)
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Microppose
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100796 - 03/10/11 09:19 PM (13 years, 10 months ago) |
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If it's contaminated, then what does it matter?  Isolate, then inoculate.
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cybernoodle2000
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Microppose]
#14100824 - 03/10/11 09:22 PM (13 years, 10 months ago) |
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Quote:
Azurascender said: If it's contaminated, then what does it matter?  Isolate, then inoculate.
I am aware of the proper procedures. However as this is a first time effort. I would just for now, like to know if that stuff is infact mycelium? Sheesh its a simple question
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Stains Blue
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100881 - 03/10/11 09:29 PM (13 years, 10 months ago) |
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Quote:
cybernoodle2000 said: I just want to know if whats growing on the bottom of the jar looks like pan mycelium.
Ohhh you think the blob crap is mycelium?

That is Caramelized Honey from the PC process. that is not mycelium.
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Doc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14100945 - 03/10/11 09:37 PM (13 years, 10 months ago) |
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Quote:
cybernoodle2000 said: I would just for now, like to know if that stuff is infact mycelium? Sheesh its a simple question 
How would we know? You're right there and you can't tell. Did it grow in the jar? Then it's something, isn't it? So shoot a test jar. But cloudy = bacteria usually.
-------------------- You make it all possible. Doesn't it feel good?
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Stains Blue
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Doc_T]
#14100975 - 03/10/11 09:40 PM (13 years, 10 months ago) |
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Quote:
Doc_T said: cloudy = bacteria usually.
yup yup
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cybernoodle2000
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Doc_T]
#14101024 - 03/10/11 09:48 PM (13 years, 10 months ago) |
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Quote:
Doc_T said:
Quote:
cybernoodle2000 said: I would just for now, like to know if that stuff is infact mycelium? Sheesh its a simple question 
How would we know? You're right there and you can't tell. Did it grow in the jar? Then it's something, isn't it? So shoot a test jar. But cloudy = bacteria usually.
After the jar cooled there was no sediment. So it would appear that stuff grew in the jar. I figured someone might be able to tell by the pics. I cant tell, because im a noob lol. As I understand Pan. mycielium is very fine and delicate. This likely only makes it harder to tell by looking, than say with cubes.
Plus honey is amber, that stuff in there is mostly white.
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Stains Blue
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14101066 - 03/10/11 09:55 PM (13 years, 10 months ago) |
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I never used Honey I use LME/Dextrose, So I cannot say anything about Honey. But I always get sediment that look EXACTLY like that on the bottom of my LC Jars after Pressure Cooking. From caramelization of the sugars.
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cybernoodle2000
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Registered: 02/11/11
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Last seen: 12 years, 5 months
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: Stains Blue]
#14101125 - 03/10/11 10:04 PM (13 years, 10 months ago) |
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Quote:
Stains Blue said: I never used Honey I use LME/Dextrose, So I cannot say anything about Honey. But I always get sediment that look EXACTLY like that on the bottom of my LC Jars after Pressure Cooking. From caramelization of the sugars.
Well, perhaps it took awhile before the sugars in the honey to carmelize? But then again the fact that my jar went from amber to almost clear suggests that something is using nutrients. Guess I will just have wait until I can test a jar.
BTW the same print was used to make these jars.
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Doc_T
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Registered: 03/06/09
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Loc: Colorado
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Re: LC, Is this Panaeolus cinctulus mycelium? [Re: cybernoodle2000]
#14102531 - 03/11/11 05:45 AM (13 years, 10 months ago) |
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Quote:
cybernoodle2000 said: After the jar cooled there was no sediment. So it would appear that stuff grew in the jar. I figured someone might be able to tell by the pics.
It's something growing in a jar. Shoot a test jar.
Also, go get a pack of agar for $1.69, it eliminates all this guesswork.
-------------------- You make it all possible. Doesn't it feel good?
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