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wickedgert
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Registered: 02/01/11
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what is the golden muck in the bottom of the jars?
#14094135 - 03/09/11 06:22 PM (12 years, 10 months ago) |
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my jars are about a month old and almost fully colonized but they have slowed a good deal and there is what looks like a very small amount of caramelized sugar in the bottom..........full pint jars not half.can anyone give me some advice please?
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RogerRabbit
Bans for Pleasure



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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14094146 - 03/09/11 06:24 PM (12 years, 10 months ago) |
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Sounds like bacteria from anaerobic conditions in the bottom of the jar. You were supposed to use 1/2 pint jars if using brown rice flour. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Mushroom Wisperer
Embrace the Martian



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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14094161 - 03/09/11 06:26 PM (12 years, 10 months ago) |
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^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Edited by Mushroom Wisperer (03/09/11 06:33 PM)
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wickedgert
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Registered: 02/01/11
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Re: what is the golden muck in the bottom of the jars? [Re: RogerRabbit]
#14100039 - 03/10/11 07:29 PM (12 years, 10 months ago) |
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so should i flip?
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phaseflux
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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14100346 - 03/10/11 08:15 PM (12 years, 10 months ago) |
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you should start over if they infected with bacteria. Chuck those ones and start anew.
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PsylioSynethesis
Experimentalist



Registered: 04/15/10
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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14100412 - 03/10/11 08:26 PM (12 years, 10 months ago) |
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Pint jars are fine, just follow the sterilization and incubation procedures closer next time. But yeh- time to toss 'em.
Was there any stage that you think you may have skipped or didn't do as well as you could. Knowing where to start next time is half the battle.
-------------------- P~S
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maynardsdick
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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14100416 - 03/10/11 08:27 PM (12 years, 10 months ago) |
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you can use pint jars but you have to increase the amount of spore solution/liquid culture to ensure the mycelium is able to engulf the substrate before anything else does. that is why most people use half-pint jars.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: what is the golden muck in the bottom of the jars? [Re: maynardsdick]
#14100439 - 03/10/11 08:31 PM (12 years, 10 months ago) |
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Quote:
maynardsdick said: you can use pint jars but you have to increase the amount of spore solution/liquid culture to ensure the mycelium is able to engulf the substrate before anything else does. that is why most people use half-pint jars.
they use half pints because the tall pint jars are known to stall, its too much substrate and packs down, making it hard for the mycelium to colonize it..and they stall out
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PsylioSynethesis
Experimentalist



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Re: what is the golden muck in the bottom of the jars? [Re: biologys]
#14100503 - 03/10/11 08:41 PM (12 years, 10 months ago) |
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Never had a problem with pints. I actually use 1/2's, full pints, and anything in between. Using 4 inoculation holes and more solution does the trick.
-------------------- P~S
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maynardsdick
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Re: what is the golden muck in the bottom of the jars? [Re: biologys]
#14100738 - 03/10/11 09:11 PM (12 years, 10 months ago) |
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Quote:
biologys said:
Quote:
maynardsdick said: you can use pint jars but you have to increase the amount of spore solution/liquid culture to ensure the mycelium is able to engulf the substrate before anything else does. that is why most people use half-pint jars.
they use half pints because the tall pint jars are known to stall, its too much substrate and packs down, making it hard for the mycelium to colonize it..and they stall out
tall pints are known to stall because they don't increase the amount of spore solution/liquid culture to compensate for the increase in volume.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: what is the golden muck in the bottom of the jars? [Re: maynardsdick]
#14100767 - 03/10/11 09:16 PM (12 years, 10 months ago) |
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it has nothing to do with increasing the amount of spores
i use 1cc in my grain quart jars i guess that means they are subject to contams since i use the same amount as a 1/2 pint jar is called for?
nope thats not how it works.
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i GrOw StUFF
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Re: what is the golden muck in the bottom of the jars? [Re: maynardsdick]
#14100795 - 03/10/11 09:19 PM (12 years, 10 months ago) |
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Quote:
maynardsdick said:
Quote:
biologys said:
Quote:
maynardsdick said: you can use pint jars but you have to increase the amount of spore solution/liquid culture to ensure the mycelium is able to engulf the substrate before anything else does. that is why most people use half-pint jars.
they use half pints because the tall pint jars are known to stall, its too much substrate and packs down, making it hard for the mycelium to colonize it..and they stall out
tall pints are known to stall because they don't increase the amount of spore solution/liquid culture to compensate for the increase in volume.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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maynardsdick
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Re: what is the golden muck in the bottom of the jars? [Re: biologys]
#14100834 - 03/10/11 09:23 PM (12 years, 10 months ago) |
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one cc in a grain jar is serviceable, but i like to add more to increase the colonization process. likewise with the pf tek when using pint rather than 1/2 pint jars. if the pf tek asks for 1cc for every injection port on 1/2 pint jars, you should double it for pint jars. not absolutely necessary, but it certainly helps with colonization times.
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wickedgert
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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14101595 - 03/10/11 11:24 PM (12 years, 10 months ago) |
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plenty of spore solution......pc may have been a little dry tho.i think its actually myc piss.
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Itheus
Tsigolocym emos



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Re: what is the golden muck in the bottom of the jars? [Re: wickedgert]
#14101628 - 03/10/11 11:35 PM (12 years, 10 months ago) |
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If you use well made lids with Tyvek and high temp. silicone, you can very safely use less than 1cc of good mycelium culture when inoculating quart jars, with only one inoculation port. I always thought multiple inoculations in one jar was silly, especially if you just want faster colonization. If you have 4 inoculation ports in one jar, your risk of contamination is quadrupled. But mostly it's a waste of time and effort, because one gets the job done just as well. If you want "faster" colonization times, you should just inoculate a few jars a week, or every few days. Plan ahead, so you always have spawn that is ready to use.
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PsylioSynethesis
Experimentalist



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Re: what is the golden muck in the bottom of the jars? [Re: Itheus]
#14101700 - 03/10/11 11:53 PM (12 years, 10 months ago) |
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Well, it doesn't get the job done "just as well with one". It is, in fact, faster to use more sites and more solution to inoculate. In my opinion and experience, having more holes and more solution counteracts the increased risk of contamination. If you are good on sterilization, the faster colonization rate will help outrace contams.
Just my 2 cents, but I've only once ever had a problem with contams pre-birthing(which is a crazy statistic if I do say so myself).
-------------------- P~S
Edited by PsylioSynethesis (03/10/11 11:54 PM)
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
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Re: what is the golden muck in the bottom of the jars? [Re: maynardsdick]
#14102190 - 03/11/11 02:24 AM (12 years, 10 months ago) |
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Quote:
maynardsdick said:
Quote:
biologys said:
Quote:
maynardsdick said: you can use pint jars but you have to increase the amount of spore solution/liquid culture to ensure the mycelium is able to engulf the substrate before anything else does. that is why most people use half-pint jars.
they use half pints because the tall pint jars are known to stall, its too much substrate and packs down, making it hard for the mycelium to colonize it..and they stall out
tall pints are known to stall because they don't increase the amount of spore solution/liquid culture to compensate for the increase in volume.
by this logic you have to use a whole syringe of spores for a bag of rye. lol
-------------------- AMU - AMU Q & A - MyVideo Teks!
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k00laid
NEMO


Registered: 05/03/10
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Re: what is the golden muck in the bottom of the jars? [Re: RogerRabbit]
#14102194 - 03/11/11 02:25 AM (12 years, 10 months ago) |
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Quote:
wickedgert said: i think its actually myc piss.
MYC PISS IS CAUSED BY BACTERIA
Quote:
RogerRabbit said: Sounds like bacteria from anaerobic conditions in the bottom of the jar. You were supposed to use 1/2 pint jars if using brown rice flour. RR
-------------------- AMU - AMU Q & A - MyVideo Teks!
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wickedgert
Stranger

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Re: what is the golden muck in the bottom of the jars? [Re: k00laid]
#14102291 - 03/11/11 03:13 AM (12 years, 10 months ago) |
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thanks guys i think ill just wait and see what happens.
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