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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
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Re: Window for my Mono Tub, PLEASE HELP URGENT!!!! Running out of time,leaving soon! [Re: biologys]
#14088810 - 03/08/11 08:19 PM (12 years, 11 months ago) |
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Made my lid tonight from the bottom of an identical stacking tote and tested my PC, stayed at 15PSI 160F for 90 minutes no problem. Tomorrow I rinse add gypsum, soak rye,finish my self healing lids, add polyfil vent and finish my mono tub. Next day I boil and shake mix with sub and then fill jars and PC them, if I get done soon enough I will inoc. Who am I kidding I am staying up till they are prego. 10 more days after tomorrow I will be able to peek at them.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 9 months
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Progress [Re: 3n1gm4]
#14091590 - 03/09/11 09:54 AM (12 years, 11 months ago) |
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Rinsed Rye and it is soaking now with gypsum. I finished my monotub except for the trash bag at the bottom, I got plenty of time before I need that. Started making some self healing lids that I am going to use polyfil as a GE filter, drilled the hole pretty big for the GE hole, scratched the area around the noc hole so the high temp rtv would stick. I guess tomorrow I will be PCing and nocing six rye jars. I just hope I have enough verm left to do both spawn and sub.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Progress [Re: 3n1gm4]
#14091745 - 03/09/11 10:32 AM (12 years, 11 months ago) |
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Quote:
3n1gm4 said: Rinsed Rye and it is soaking now with gypsum. I finished my monotub except for the trash bag at the bottom, I got plenty of time before I need that. Started making some self healing lids that I am going to use polyfil as a GE filter, drilled the hole pretty big for the GE hole, scratched the area around the noc hole so the high temp rtv would stick. I guess tomorrow I will be PCing and nocing six rye jars. I just hope I have enough verm left to do both spawn and sub.
Polyfil is NOT a contam barrier. Use tyvek or SFD or E-Z-Felt.
I've never scratched my lids to get the RTV to adhere. It does so w/out scratching, just tryin' to save ya some time brotha.
How big is your GE hole?
Also, you don't HAVE to put a trash bag in a mono, it just helps w/ side pins.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Quote:
todlow said:
Quote:
3n1gm4 said: Rinsed Rye and it is soaking now with gypsum. I finished my monotub except for the trash bag at the bottom, I got plenty of time before I need that. Started making some self healing lids that I am going to use polyfil as a GE filter, drilled the hole pretty big for the GE hole, scratched the area around the noc hole so the high temp rtv would stick. I guess tomorrow I will be PCing and nocing six rye jars. I just hope I have enough verm left to do both spawn and sub.
Polyfil is NOT a contam barrier. Use tyvek or SFD or E-Z-Felt.
I've never scratched my lids to get the RTV to adhere. It does so w/out scratching, just tryin' to save ya some time brotha.
How big is your GE hole?
Also, you don't HAVE to put a trash bag in a mono, it just helps w/ side pins.
alot of ppl use polyfil as a filter in their grain jars....with great success
I used to as well, just found tyvek to be alot easier and simplier..
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
biologys said:
Quote:
todlow said:
Quote:
3n1gm4 said: Rinsed Rye and it is soaking now with gypsum. I finished my monotub except for the trash bag at the bottom, I got plenty of time before I need that. Started making some self healing lids that I am going to use polyfil as a GE filter, drilled the hole pretty big for the GE hole, scratched the area around the noc hole so the high temp rtv would stick. I guess tomorrow I will be PCing and nocing six rye jars. I just hope I have enough verm left to do both spawn and sub.
Polyfil is NOT a contam barrier. Use tyvek or SFD or E-Z-Felt.
I've never scratched my lids to get the RTV to adhere. It does so w/out scratching, just tryin' to save ya some time brotha.
How big is your GE hole?
Also, you don't HAVE to put a trash bag in a mono, it just helps w/ side pins.
alot of ppl use polyfil as a filter in their grain jars....with great success
I used to as well, just found tyvek to be alot easier and simplier..
Just because a lot of people use it doesn't make it true. Polyfil is not a filtering media. It is a AE restricting media. https://www.shroomery.org/forums/showflat.php/Number/12543108 I just got into this same discussion on another thread. People do use it as a filter media, but it's not meant for such use. Those people that do use it & don't get contams are extremely lucky.
I don't know why everyone wants to take short cuts on things that you shouldn't skimp on. Sterile issues are something I never skip on. &, in all honesty, $12 for 12 100mm SFD is a good deal considering that's 60-72 jars...that the filter media will never go bad on...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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it's a debate that I myself am not getting into...i use tyvek so im not going to argue about it lol
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
biologys said: it's a debate that I myself am not getting into...i use tyvek so im not going to argue about it lol
I don't want to get into it either. It's going on in the other thread...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 9 months
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My GE hole is 3/8 inch, my noc hole is 1/4 inch.
RR or someone said yesterday that the tyvek from envelopes is crap because it is so thin, I dont have the time or money to order filter disks, all of the paint suits looked more like cotton, they said that they block up to .3 microns dry but they looked like they would hold moisture, which would be bad.
Bio do you know any alternative contam barrier/GE filter I could use that you can find locally pretty easy and cheap, like some kind of medical disc from a drug store or something? Could I just use bandaids doubled up in an X pattern. Or would the paint suits that aren't tyvek work? Otherwise, if I can't find something local and cheap in the next few days then polyfil will be my only option.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
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Last seen: 12 years, 9 months
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Re: Progress [Re: 3n1gm4]
#14091965 - 03/09/11 11:18 AM (12 years, 11 months ago) |
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Quote:
RogerRabbit said: I doubt they (non tyvek paint suits)would retain that .3 micron rating after autoclaving. In fact, tyvek often opens up the pores, or closes them off in the sterilizer. I no longer use it for jar filters for that reason. Tyvek envelopes are even worse because they're so thin.
Order some synthetic filter disks, or use polyfill. RR
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Quote:
todlow said:
Quote:
biologys said: it's a debate that I myself am not getting into...i use tyvek so im not going to argue about it lol
I don't want to get into it either. It's going on in the other thread...
Iv made at least 100+ 1L jars using polyfil and I have not had a single contaminate because of it.
That goes beyond being lucky, when used properly polyfil is an effective method for GE in your jars.
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
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Is it really that important? I just don't see any contam spores landing on my polyfil filter on a shelf in my closet and working it's way down through it.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Progress [Re: 3n1gm4]
#14092041 - 03/09/11 11:31 AM (12 years, 11 months ago) |
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I used polyfil for ALL my jars up until last week when a generous member sent me some SFD to try out. Like I said above I have never had a contaminate attributed to the polyfil filter.
If you have the extra $$ spring for some SFD for sure, but for you to say(todlow) that anyone who uses polyfil without contaminates is lucky does not hold any water with me. When used properly and snugged into a hole using a pair of pliers it has worked 100% of the time for me. That is a pretty high percentage for luck.
I must say I am hooked on SFD now for ease of use and re-usability, but polyfil will always hold a place in my heart.
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k00laid
NEMO


Registered: 05/03/10
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Last seen: 7 months, 6 days
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ye im a polyfil kinda guy.
and 100% success rate.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Progress [Re: 3n1gm4]
#14092076 - 03/09/11 11:38 AM (12 years, 11 months ago) |
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Quote:
3n1gm4 said:
Bio do you know any alternative contam barrier/GE filter I could use that you can find locally pretty easy and cheap, like some kind of medical disc from a drug store or something? .
E-Z-Felt from a local hobby store cut to size...3/8" is fine. That's what I use. I've got a couple of jars w/ 1/2" & others w/ (2) 1/4" holes. I've noticed no difference between the 3 styles.
Evil - I understand people use it as filter material, I just don't feel as though the benefits outweigh the risks. Besides, w/ SFD's they're done once they're siliconed to the lid. Never mess w/ them again & no worries about contams. How those Whatmans turn out for ya?
& whatever on the polyfil, if it works for you guys, it works, but I'm not going to tell anyone to use it as filter media...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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all of my agar to LC are clean so far, and i use polyfil in them 
i just like the ease of tyvek(envelopes) for grain jars, and have great success with it..
but just as you said evil i got some SFD in a trade and im going to make about 20 jars tonight with them and silicon to test them out
i have some nice shiitake and p. mexicana LC's that i need to get started using in some jars ;p
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Quote:
todlow said:
Evil - I understand people use it as filter material, I just don't feel as though the benefits outweigh the risks. Besides, w/ SFD's they're done once they're siliconed to the lid. Never mess w/ them again & no worries about contams. How those Whatmans turn out for ya?
& whatever on the polyfil, if it works for you guys, it works, but I'm not going to tell anyone to use it as filter media...
I just personally have not seen these risks you speak of, to each there own but IMO there is more then one way to skin a cat.
As for the whatmans, I actually have gifted them out as bonus's in a lot of my trades so only have a few left to play with. Next time I make an LC I will let you know how it goes, I am sure it will be good as I have heard nothing but good things about the whatman filters.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
biologys said: all of my agar to LC are clean so far, and i use polyfil in them 
i just like the ease of tyvek(envelopes) for grain jars, and have great success with it..
but just as you said evil i got some SFD in a trade and im going to make about 20 jars tonight with them and silicon to test them out
i have some nice shiitake and p. mexicana LC's that i need to get started using in some jars ;p
Cut them to size...You will be very happy. I use my cutting head for my dremel tool to trace a perfect circle that covers my 3/8".
Evil - me if ya need more, I haven't forgotten what originally happened . I still have a few hundred extra (haven't even opened my second box, actually). I have absolutely NO problem w/ them in my LC's. Actually, I just made up some 1/4 pint agar dishes for clones the other day w/ the Whatmans too. I've PC'd some of the Whatmans twice & they're fine...if you were worried about that
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 9 months
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Quote:
todlow said:
Quote:
3n1gm4 said:
Bio do you know any alternative contam barrier/GE filter I could use that you can find locally pretty easy and cheap, like some kind of medical disc from a drug store or something? .
E-Z-Felt from a local hobby store cut to size...3/8" is fine. That's what I use. I've got a couple of jars w/ 1/2" & others w/ (2) 1/4" holes. I've noticed no difference between the 3 styles.
Evil - I understand people use it as filter material, I just don't feel as though the benefits outweigh the risks. Besides, w/ SFD's they're done once they're siliconed to the lid. Never mess w/ them again & no worries about contams. How those Whatmans turn out for ya?
& whatever on the polyfil, if it works for you guys, it works, but I'm not going to tell anyone to use it as filter media...
Does the E-Z felt protect rating protect to smaller than .6 microns, or do you even know. Thank you BTW, I didn't have an alternative because my town hates tyvek or something but now I do.
Also can someone link me that thread where they are discussing this so I can kill this thread.
Quote:
EvilMushroom666 said:
Quote:
todlow said:
Evil - I understand people use it as filter material, I just don't feel as though the benefits outweigh the risks. Besides, w/ SFD's they're done once they're siliconed to the lid. Never mess w/ them again & no worries about contams. How those Whatmans turn out for ya?
& whatever on the polyfil, if it works for you guys, it works, but I'm not going to tell anyone to use it as filter media...
I just personally have not seen these risks you speak of, to each there own but IMO there is more then one way to skin a cat.
As for the whatmans, I actually have gifted them out as bonus's in a lot of my trades so only have a few left to play with. Next time I make an LC I will let you know how it goes, I am sure it will be good as I have heard nothing but good things about the whatman filters.
As for the filter disks I couldn't tell if they were the right kind but they were lab disks, I know the ones at everything mushroom, but I will be cooking the grains and PCing them tomorrow, SO, if I just use a glovebox and sterile procedures, I should be okay with the polyfil I think.
The worst thing is that I have never done this and if I did read how tight to pack it I think it said medium tight"use your judgment". But my polyfil is from a new pillow and it is very hard to get it to stuff in tight. I got it to look like the bubble shape on the inside but it still felt loose, I wish there was a way to roll it in your fingers and tell if the polyfil jar filter was the perfect tightness before you put it in there. Can you Twist it? I don't wana go fondle a brand new pillow.
I know I that I have a thread on this I posted the original question on wrong thread or it just got changed in the middle or something, don't feel like reading it. I will just make a question post and a Progress post and change the name of the first post so that I am not double and multi posting.
Quote:
todlow said:
Quote:
biologys said: all of my agar to LC are clean so far, and i use polyfil in them 
i just like the ease of tyvek(envelopes) for grain jars, and have great success with it..
but just as you said evil i got some SFD in a trade and im going to make about 20 jars tonight with them and silicon to test them out
i have some nice shiitake and p. mexicana LC's that i need to get started using in some jars ;p
Cut them to size...You will be very happy. I use my cutting head for my dremel tool to trace a perfect circle that covers my 3/8".
Evil - me if ya need more, I haven't forgotten what originally happened . I still have a few hundred extra (haven't even opened my second box, actually). I have absolutely NO problem w/ them in my LC's. Actually, I just made up some 1/4 pint agar dishes for clones the other day w/ the Whatmans too. I've PC'd some of the Whatmans twice & they're fine...if you were worried about that
Could I use a bandaid? WIll that EZ stuff keep out bacteria, and mold spores?
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Progress [Re: 3n1gm4]
#14092315 - 03/09/11 12:30 PM (12 years, 11 months ago) |
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Just because it came out of a pillow, does not mean it is polyfil first off.
Goto a craft store and get a big bag of polyfil for $2-5. Then you KNOW it is polyfil and not some other material/cotton.
IMO band aids will not work.
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 9 months
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Re: Progress [Re: 3n1gm4]
#14092357 - 03/09/11 12:40 PM (12 years, 11 months ago) |
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" You just squeeze THIS HARD." LOL I wish I gould thing of a way to compare the tightness to something, (like maybe a foam stress ball or a pair of folded socks), how tight you should have it at on my holes during colonization and then about how loose to make it for my monotub holes.
I don't even know to judge how tight I should make the GE hole I need to make a test hole and practice it or something, guess I could practice the mono's holes too.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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