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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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RR ????
#14091707 - 03/09/11 10:25 AM (13 years, 10 months ago) |
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when i shake @ 20-30% on my grains...i seem to see the contams soon after..is it my spawn,old lids,bad prep??? i still have 75% do fine.i use a glove box..no hood. i have one more?..if i get a single piece of grass growing on my bulk w/straw..does that mean i screwed up on my pasteurize tek?? only a few contams so far.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: RR ???? [Re: penhed]
#14091719 - 03/09/11 10:27 AM (13 years, 10 months ago) |
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if you have a contam in the jar, not long after you shake is when its going to show..
it could be your sterilization proces, or your syringes or your lids...
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shroomtastic89
Dolla Dolla Bills Y'all



Registered: 11/28/10
Posts: 573
Loc: USA
Last seen: 7 years, 10 months
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Yea hard telling where it came from... Carefully retrace your steps and look at each little detail and improve from what you did this time for your next grow...
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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its g2g and seems if i do not shake..they do better..same master with 1-2 bad jars???sort of just got used to it.but still....why??
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: RR ???? [Re: penhed]
#14091763 - 03/09/11 10:35 AM (13 years, 10 months ago) |
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when you shake, you are 'weakening' the myc, and in its recovering time it allows the trich to take over.
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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so the jars that i did not shake have contams,but the myc has the upper hand?? just a matter of time before it shows?? thanks biologys
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: RR ???? [Re: penhed]
#14091798 - 03/09/11 10:42 AM (13 years, 10 months ago) |
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Exactly.
How are you inoculating your grain masters?
-------------------- You make it all possible. Doesn't it feel good?
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shroomtastic89
Dolla Dolla Bills Y'all



Registered: 11/28/10
Posts: 573
Loc: USA
Last seen: 7 years, 10 months
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Re: RR ???? [Re: penhed]
#14091799 - 03/09/11 10:43 AM (13 years, 10 months ago) |
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Theres no real way to tell if they are contamed until you notice some mold... It's nothing to sweat about it happens to all of us... Just try to read up a little more on sterile inoculation and give it another shot...
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LightShedder
Cultivator



Registered: 08/30/05
Posts: 3,283
Loc: Shroomecca
Last seen: 27 days, 21 hours
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Since it's g2g I'd bet on it being your inoculation process. I use lc and syringes in a glovebox and while that works 100%, I have little luck with g2g in the same glovebox. It seems like you're not seeing what is probably a tiny amount of contam somewhere in there prior to shaking. Maybe during the grain transfer the lid becomes contaminated. Or it could be in the jar on the grain, either way, I'd suggest finding a different inoculation method if you wanna stick with the glovebox. Agar to LC is a simple way to generate tons of culture and believe me in a glovebox, lc works amazingly.
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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whats up DOC i first did a ms to karo lc in dec. then lc to grains, g2g since...i started some new masters a few days ago. a will have agar soon..as i know you dont care for lcs . it has been good to me so far...i think my refriged lc are good..just like my g2gs..thankx
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: RR ???? [Re: penhed]
#14091907 - 03/09/11 11:05 AM (13 years, 10 months ago) |
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LC 
You have a small subtle contam in an otherwise nearly-good LC. Toss it, start over with spores on agar.
I love LC, but I'm ruthless in controlling it. If you fall into the trap of thinking they are good without proof, you'll fail. And even one that seems good can be bad, as you've shown us all.
Don't believe it's the LC? You can prove it. Do side-by-side g2g, one from a suspect master, one from a known-good source. Your LC-based ones will contam while the others do not.
-------------------- You make it all possible. Doesn't it feel good?
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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Re: RR ???? [Re: Doc_T]
#14091955 - 03/09/11 11:15 AM (13 years, 10 months ago) |
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i use brf cakes to test my lcs,but even if the wife flips out..i'm order'n some agar right now...no more play time...we will take turns eating next week..priorities are agar
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: RR ???? [Re: penhed]
#14091960 - 03/09/11 11:16 AM (13 years, 10 months ago) |
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Don't order agar, go get it at the asian market for $1.69... in three colors.
-------------------- You make it all possible. Doesn't it feel good?
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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Re: RR ???? [Re: Doc_T]
#14091997 - 03/09/11 11:22 AM (13 years, 10 months ago) |
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ok ...i also know the local beer supply has most stuff to mix up..just been content with my grows so far..but tired of throw'n out grains once a week,,even though the birds love me
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: RR ???? [Re: penhed]
#14092016 - 03/09/11 11:26 AM (13 years, 10 months ago) |
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Quote:
penhed said: when i shake @ 20-30% on my grains...i seem to see the contams soon after..is it my spawn,old lids,bad prep??? i still have 75% do fine.i use a glove box..no hood. i have one more?..if i get a single piece of grass growing on my bulk w/straw..does that mean i screwed up on my pasteurize tek?? only a few contams so far.
Shaking does not cause contamination, but it will let you know if you have it in your grains or not, because if your grain is contaminated with molds or bacteria it won't recover.
Quote:
penhed said: i first did a ms to karo lc in dec. then lc to grains, g2g since...
I've posted here a few hundred times about what a waste of time making LC is. If you wish to use outdated and proved inferior procedures, you're on your own. Here it is mid-March and you made that silly LC in December. If you had used reliable procedures you'd have harvested mushrooms by now. Sorry I can't be any more help, but you'll have to follow proper procedure if you wish to have a high probability of success.
There's a very good reason why there is no liquid culture tek on Let's Grow Mushrooms.
If you get a single blade of grass growing on straw, it means you didn't pasteurize properly. Seeds are killed at a temperature of less than 140F/60C. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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i have harvested approx. three lbs since jan. if the info for lc was not here ...i would not had my success .i will move on to better methods ..thankx
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