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OfflineMinty
Shroom Girl
Female


Registered: 02/07/11
Posts: 17
Last seen: 12 years, 6 months
Oh no! Trichoderma! *DELETED*
    #14089632 - 03/08/11 10:29 PM (12 years, 10 months ago)

Post deleted by Minty

Reason for deletion: It wasn't trichoderma.



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InvisibleEvilMushroom666
Heretic
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Posts: 10,289
Loc: Canada Flag
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Re: Oh no! Trichoderma! [Re: Minty]
    #14089657 - 03/08/11 10:33 PM (12 years, 10 months ago)

How did you prepare your grains?

Vectors of Contamination:
-Improper Sterilization
-Contamination of Inoculate (Spore Syringe)
-Improper Filtered Gas Exchange(What is your lid setup?)

Now that you know you have the contaminate you must trace your steps
back and dis-assemble your procedures to find the vector of contamination.

If you properly sterilized your jars, and have a proper lid design
for GE chances are you had/have a contaminated spore syringe.


If you want to save the jars PC them for 90 minutes at 15 PSI and then
empty the contents into the trash. Wash the jars with soap and water
and try again.


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InvisibleDoc_T
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Re: Oh no! Trichoderma! [Re: Minty]
    #14089664 - 03/08/11 10:34 PM (12 years, 10 months ago)

Is is in the places you inoculated?
If so, it's in the syringe.
If the green does not follow the injection pattern, then it's probably your jar prep.

What kind of jars? What are you shooting? Where's you get it?

You can't save the jars. Empty them outdoors, wash them out and start new ones.


--------------------
You make it all possible. Doesn't it feel good?


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InvisibleJavadog
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Re: Oh no! Trichoderma! [Re: Doc_T]
    #14089717 - 03/08/11 10:42 PM (12 years, 10 months ago)

"If the green does not follow the injection pattern, then it's probably your jar prep."

Excellent point Doc.

I have found syringes to be infected with bacteria often, and have gotten
prints that seemed to be 100% trich (they were exotic wild finds,
bought from, and generously replaced by, SW), but your point remains valid.

So, lets see what the materials and methods were.

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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InvisibleDoc_T
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Re: Oh no! Trichoderma! [Re: Javadog]
    #14089788 - 03/08/11 10:52 PM (12 years, 10 months ago)

My ability to track contams is a leftover skill from being a computer programmer. :wink:

But I'm betting on the syringe, there seems to be a rash of bad syringes out there.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineHitsuzen
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Re: Oh no! Trichoderma! [Re: Doc_T]
    #14089834 - 03/08/11 10:57 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Is is in the places you inoculated?
If so, it's in the syringe.
If the green does not follow the injection pattern, then it's probably your jar prep.





That is damned good reasoning... :thumbup:


--------------------
Hate never yet dispelled hate,
Only love dispels hate.
This is the law,
Ancient and inexhaustible.
-Buddha


                           


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