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Minty
Shroom Girl



Registered: 02/07/11
Posts: 17
Last seen: 12 years, 6 months
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Oh no! Trichoderma! *DELETED*
#14089632 - 03/08/11 10:29 PM (12 years, 10 months ago) |
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Post deleted by MintyReason for deletion: It wasn't trichoderma.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Oh no! Trichoderma! [Re: Minty]
#14089657 - 03/08/11 10:33 PM (12 years, 10 months ago) |
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How did you prepare your grains?
Vectors of Contamination: -Improper Sterilization -Contamination of Inoculate (Spore Syringe) -Improper Filtered Gas Exchange(What is your lid setup?)
Now that you know you have the contaminate you must trace your steps back and dis-assemble your procedures to find the vector of contamination.
If you properly sterilized your jars, and have a proper lid design for GE chances are you had/have a contaminated spore syringe.
If you want to save the jars PC them for 90 minutes at 15 PSI and then empty the contents into the trash. Wash the jars with soap and water and try again.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Oh no! Trichoderma! [Re: Minty]
#14089664 - 03/08/11 10:34 PM (12 years, 10 months ago) |
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Is is in the places you inoculated? If so, it's in the syringe. If the green does not follow the injection pattern, then it's probably your jar prep.
What kind of jars? What are you shooting? Where's you get it?
You can't save the jars. Empty them outdoors, wash them out and start new ones.
-------------------- You make it all possible. Doesn't it feel good?
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Oh no! Trichoderma! [Re: Doc_T]
#14089717 - 03/08/11 10:42 PM (12 years, 10 months ago) |
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"If the green does not follow the injection pattern, then it's probably your jar prep."
Excellent point Doc.
I have found syringes to be infected with bacteria often, and have gotten prints that seemed to be 100% trich (they were exotic wild finds, bought from, and generously replaced by, SW), but your point remains valid.
So, lets see what the materials and methods were.
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Oh no! Trichoderma! [Re: Javadog]
#14089788 - 03/08/11 10:52 PM (12 years, 10 months ago) |
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My ability to track contams is a leftover skill from being a computer programmer. 
But I'm betting on the syringe, there seems to be a rash of bad syringes out there.
-------------------- You make it all possible. Doesn't it feel good?
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Hitsuzen
Space Cowboy



Registered: 10/17/08
Posts: 399
Loc: Earth
Last seen: 3 years, 2 months
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Re: Oh no! Trichoderma! [Re: Doc_T]
#14089834 - 03/08/11 10:57 PM (12 years, 10 months ago) |
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Quote:
Doc_T said: Is is in the places you inoculated? If so, it's in the syringe. If the green does not follow the injection pattern, then it's probably your jar prep.
That is damned good reasoning...
-------------------- Hate never yet dispelled hate, Only love dispels hate. This is the law, Ancient and inexhaustible. -Buddha
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