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OfflineiSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 11 years, 27 days
Q About flame sterilization
    #14085854 - 03/08/11 09:44 AM (12 years, 11 months ago)

Im about to noc up some jars and i only have a bic lighter to flame my needle.

the last time i did this i notice the needle tip turning black. So id flame, inject and then wipe with a cloth.

Then flame again for new jar.

Is that black stuff going to damage my babies!?!?!


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Pain is just weakness leaving the body

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InvisibleEvilMushroom666
Heretic
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Folding@home Statistics
Registered: 11/18/09
Posts: 10,289
Loc: Canada Flag
Trusted Cultivator
Re: Q About flame sterilization [Re: iSHACKA]
    #14085867 - 03/08/11 09:49 AM (12 years, 11 months ago)

Flame sterilization with a BIC lighter is just fine.

If you do not want to deal with soot I would suggest purchasing a
propane torch with a fatboy tank(For added stability). I spent under
$20 and got the adapter and a 17Oz(?) tank. So far it has been months
and it is still going strong.

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Offlinekr0n1x
Stranger

Registered: 03/08/11
Posts: 4
Last seen: 10 months, 2 days
Re: Q About flame sterilization [Re: EvilMushroom666]
    #14085879 - 03/08/11 09:53 AM (12 years, 11 months ago)

another option would be a denatured alcohol lamp.  10 bucks on ebay.

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Invisiblebootster
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Registered: 02/22/11
Posts: 1,531
Re: Q About flame sterilization [Re: EvilMushroom666]
    #14085880 - 03/08/11 09:54 AM (12 years, 11 months ago)

These are the best IMO

Mini Torch

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OfflineCynosure
allow me to be your guide.
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Registered: 10/06/09
Posts: 4,228
Last seen: 1 year, 20 days
Re: Q About flame sterilization [Re: iSHACKA]
    #14085882 - 03/08/11 09:54 AM (12 years, 11 months ago)

I get the same effect when using a lighter to sterilize my needles. 

All of my jars have colonized perfectly fine with no contaminants or trace of soot in the substrate.

Through experimenting, I found that wiping the needle down with an alcohol pad before the next flame sterilization greatly reduces the amount of buildup on the needle.  I even wipe it off sometimes after I flame sterilize and let it dry quickly (using 99% alcohol) before sticking it in an inoculation point.  This keeps the suit from getting stuck on the micropore tape.

I'm planning on upgrading to an alcohol lamp for my next inoculation, as they are cheap and hands-free.


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"You can peel it [language] off the ceiling and make it dance in front of you" - McKenna

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Offlinemagnumzero
Soon-to-be Mycologist
Male


Registered: 01/30/08
Posts: 513
Last seen: 10 years, 9 months
Re: Q About flame sterilization [Re: Cynosure]
    #14085902 - 03/08/11 10:00 AM (12 years, 11 months ago)

Quote:

(using 99% alcohol)



Believe it or not 70% is more effective.


--------------------

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Offlinemycobeginner
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Registered: 08/23/10
Posts: 831
Loc: Mushroom land
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Re: Q About flame sterilization [Re: magnumzero]
    #14085919 - 03/08/11 10:05 AM (12 years, 11 months ago)

70% is more effective because it does'nt dry nearly as fast as 99 or 90%


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OfflineCryogenicz
what?
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Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 9 months
Re: Q About flame sterilization [Re: mycobeginner]
    #14086023 - 03/08/11 10:37 AM (12 years, 11 months ago)

70% alcohol's effectivness is on its direct use on microbes, not as a flame.


The higher 30% water content helps alcohols action on killing microbes as a disinfectant.

-Graham


--------------------
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OfflineCryogenicz
what?
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Registered: 07/01/04
Posts: 2,421
Loc: Oregon
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Re: Q About flame sterilization [Re: Cryogenicz]
    #14086031 - 03/08/11 10:40 AM (12 years, 11 months ago)

On a side note, alcohol is not effective against fungal and bacterial spores...

-Graham


--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

www.FungiForum.com

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