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velvetmagician
Brewer



Registered: 03/01/11
Posts: 422
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Using a microwave for sterilization?
#14084405 - 03/07/11 11:41 PM (12 years, 10 months ago) |
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Does using the microwave to sterilize spore water and shot glass work?? Anyone ever use a microwave to sterilize anything?
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: Using a microwave for sterilization? [Re: velvetmagician]
#14084408 - 03/07/11 11:42 PM (12 years, 10 months ago) |
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microwaves have no place in mycology, and definately not in sterilization...
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3Cajun1Mo8
Fung Padawan



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Re: Using a microwave for sterilization? [Re: biologys]
#14084412 - 03/07/11 11:43 PM (12 years, 10 months ago) |
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I've used the microwave to make LC's, thats about it
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velvetmagician
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Re: Using a microwave for sterilization? [Re: 3Cajun1Mo8]
#14084415 - 03/07/11 11:44 PM (12 years, 10 months ago) |
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what was your method 3Cajun1Mo8
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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3Cajun1Mo8
Fung Padawan



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Re: Using a microwave for sterilization? [Re: velvetmagician]
#14084420 - 03/07/11 11:47 PM (12 years, 10 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/2321056/fpart/1/vc/1 Instead of Karo I used honey. And I would advise not to use seran wrap as its really annoying and can make a mess
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velvetmagician
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Re: Using a microwave for sterilization? [Re: 3Cajun1Mo8]
#14084437 - 03/07/11 11:51 PM (12 years, 10 months ago) |
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I have yet to learn and search the forum for LC. I know nothing about it.
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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Doc_T
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Loc: Colorado
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Re: Using a microwave for sterilization? [Re: velvetmagician]
#14084450 - 03/07/11 11:55 PM (12 years, 10 months ago) |
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Quote:
velvetmagician said: Anyone ever use a microwave to sterilize anything?
No, get a pressure cooker at a thrift store.
And what are you sterilizing? You don't have spores.
Also, forget about LC.
-------------------- You make it all possible. Doesn't it feel good?
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wow
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Re: Using a microwave for sterilization? [Re: Doc_T]
#14084485 - 03/08/11 12:05 AM (12 years, 10 months ago) |
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haters! i use a microwave all the time!! it sterilizezz water just fine!! just boil it harrd for a while! i have a pc too but im too lazy sometimes.. i have a new tek thats gonna make this real simple for a noob.. check my thread haterz.. http://www.shroomery.org/forums/showflat.php/Number/14049567
if i were you id sterilize the water in a tupperware with the lid cracked a lil bit.. be sterile and use a GB or still air room when you perform.. water boils VERY hot in the microwave if you let it.. just boil hard and youll be fine.. i make my spore syringes this way TOO!!
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Doc_T
Random Dude




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Re: Using a microwave for sterilization? [Re: wow]
#14084493 - 03/08/11 12:06 AM (12 years, 10 months ago) |
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Quote:
wow said: water boils VERY hot in the microwave if you let it..
No.
-------------------- You make it all possible. Doesn't it feel good?
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: wow]
#14084504 - 03/08/11 12:09 AM (12 years, 10 months ago) |
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also the biggest tip i could give you... figure things out for yourself.. seriously.. take risks.. if you have a limited number of spores be carefull.. other than that its easy...
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Doc_T
Random Dude




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Re: Using a microwave for sterilization? [Re: wow]
#14084547 - 03/08/11 12:22 AM (12 years, 10 months ago) |
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He lives in Cali, if he gets spores they'll be priceless.
Best, safest, most secure way to utilize them is with agar. Pressure cooker, some agar flakes and malt, dishes or jars... not very expensive.
-------------------- You make it all possible. Doesn't it feel good?
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wow
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Re: Using a microwave for sterilization? [Re: Doc_T]
#14084636 - 03/08/11 12:51 AM (12 years, 10 months ago) |
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Quote:
Doc_T said:
Quote:
wow said: water boils VERY hot in the microwave if you let it..
No.
im sorry.. im not hating and you know your shit doc.. but i must disagree with you!! theres a tek out there using KARO and a MICROWAVE to create a sterilized liquid culture.. if this is possible than the microwave syringe is possible! i personally put the syringe in a tupperware with water.. i suck up half water half air in the syringe.. support the syringe out of the water by leaning it on the side of the tupperware and shutting the lid on the end of the plunger and nuke the mofo for 6 minutes.... once its done push the plunger down.. suck up the hot water.. let it cool in a sterile enviromment.. than put the spores into a ziplock bag by putting the print in the bag and scraping it on the inside of the bag with my fingers (on the outside).. be careful not to let any part of the print that you touched with your fingers to touch the inside of the bag tho.. than i shoot the water into the bag to suspend the spores and suck it back into the syringe.. to each his own but thats what i do... its worked for me so far.. ALSO i make my karo LC's in the microwave too! the link i posted is to my current experiment using ONLY the microwave!! EVERYTHING involved including the LC was with the microwave!! i didnt use the stove for ANYTHING!! but if you only have a limited amount of spores and want to guarantee success.. maybe go the extra mile and spend the extra hour to do it "right".... its hard to get spores where im at too.. but you gotta go thru trial and error to develope a tek that works for you..
Edited by wow (03/08/11 12:52 AM)
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wow
Lurking master



Registered: 07/27/06
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Re: Using a microwave for sterilization? [Re: Doc_T]
#14084646 - 03/08/11 12:55 AM (12 years, 10 months ago) |
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Quote:
Doc_T said: He lives in Cali, if he gets spores they'll be priceless.
Best, safest, most secure way to utilize them is with agar. Pressure cooker, some agar flakes and malt, dishes or jars... not very expensive.
and i agree.. totally.. a solid culture can be had this way using very little spores..
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Doc_T
Random Dude




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Posts: 42,395
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Re: Using a microwave for sterilization? [Re: wow]
#14084697 - 03/08/11 01:10 AM (12 years, 10 months ago) |
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1) water boils at the same temp inside the microwave as outside 2) any boy scout can tell you boiling doesn't sterilize water.
It's that easy. Maybe you get away with it. But it's not sterilization. 
But a PC is like ten or fifteen bucks, so the whole conversation is hypothetical at best.
-------------------- You make it all possible. Doesn't it feel good?
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velvetmagician
Brewer



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Re: Using a microwave for sterilization? [Re: wow]
#14084794 - 03/08/11 01:38 AM (12 years, 10 months ago) |
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Quote:
wow said:
Quote:
Doc_T said:
Quote:
wow said: water boils VERY hot in the microwave if you let it..
No.
im sorry.. im not hating and you know your shit doc.. but i must disagree with you!! theres a tek out there using KARO and a MICROWAVE to create a sterilized liquid culture.. if this is possible than the microwave syringe is possible! i personally put the syringe in a tupperware with water.. i suck up half water half air in the syringe.. support the syringe out of the water by leaning it on the side of the tupperware and shutting the lid on the end of the plunger and nuke the mofo for 6 minutes.... once its done push the plunger down.. suck up the hot water.. let it cool in a sterile enviromment.. than put the spores into a ziplock bag by putting the print in the bag and scraping it on the inside of the bag with my fingers (on the outside).. be careful not to let any part of the print that you touched with your fingers to touch the inside of the bag tho.. than i shoot the water into the bag to suspend the spores and suck it back into the syringe.. to each his own but thats what i do... its worked for me so far.. ALSO i make my karo LC's in the microwave too! the link i posted is to my current experiment using ONLY the microwave!! EVERYTHING involved including the LC was with the microwave!! i didnt use the stove for ANYTHING!! but if you only have a limited amount of spores and want to guarantee success.. maybe go the extra mile and spend the extra hour to do it "right".... its hard to get spores where im at too.. but you gotta go thru trial and error to develope a tek that works for you..
here is where you probably read about this : http://www.shroomery.org/9145/Liquid-Culture-Basics
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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German Kahuna
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Re: Using a microwave for sterilization? [Re: wow]
#14084823 - 03/08/11 01:48 AM (12 years, 10 months ago) |
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Quote:
wow said:
haters! i use a microwave all the time!! it sterilizezz water just fine!! just boil it harrd for a while! i have a pc too but im too lazy sometimes.. i have a new tek thats gonna make this real simple for a noob.. check my thread haterz.. http://www.shroomery.org/forums/showflat.php/Number/14049567
if i were you id sterilize the water in a tupperware with the lid cracked a lil bit.. be sterile and use a GB or still air room when you perform.. water boils VERY hot in the microwave if you let it.. just boil hard and youll be fine
Your claims are in defiance of the laws of nature. If you want to raise the boiling point of water you gotta up the pressure. Works the same way the other way around. The higher up in the mountains you go, the lower the boiling point of water will be. Physics... it's amazing!
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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wow
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Re: Using a microwave for sterilization? [Re: German Kahuna]
#14084883 - 03/08/11 02:16 AM (12 years, 10 months ago) |
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whatever......... to each his own......... just stating what works for me.. and click that link.. its mine... you may be surprised....
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RogerRabbit
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Re: Using a microwave for sterilization? [Re: wow]
#14085473 - 03/08/11 07:33 AM (12 years, 10 months ago) |
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It works because you can make a karo LC without sterilizing at all. Ever seen mold growing in a bottle of karo, even when it's been in a nasty kitchen for years? 
A car without an engine can go really fast down a hill, but it wouldn't be right to call it a fast car.
Don't take the above to mean I think anyone should make LC without sterilizing. In fact, I wouldn't suggest making LC at all, regardless of preparation method. There's better and faster ways of growing. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: RogerRabbit]
#14085638 - 03/08/11 08:27 AM (12 years, 10 months ago) |
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Quote:
RogerRabbit said: It works because you can make a karo LC without sterilizing at all. Ever seen mold growing in a bottle of karo, even when it's been in a nasty kitchen for years? 
A car without an engine can go really fast down a hill, but it wouldn't be right to call it a fast car.
Don't take the above to mean I think anyone should make LC without sterilizing. In fact, I wouldn't suggest making LC at all, regardless of preparation method. There's better and faster ways of growing. RR
i HAD a karo lc with the green in it a while ago!! everything ive shot up with it has gotten green.. i think i introduced it for other reasons (plain laziness) because it worked before.. which goes to say.. a karo lc can be contam'd.. also i have karo lc's (all microwaved) that arent contaminated.. and are proven.. which also goes to say.. they can be sterilized in the microwave.. id personally say g2g is the best way.. and lc is just short of that in colonization times... i know you hate lc rr.. but in reality were talking about the water here.. so do you think that vigorously boiled water in the microwave is spore syringe worthy?? i sure as hell do.. thats what i been doin since day one.. now.. if im gonna get the pc out and fire it up ill go ahead and sterilize me some syringes that way.. its truly a pain in the ass for me to get it all out and do it just for a syringe.. plus you can boil the thing on high for 15 min if you want.. or longer.. id even bet you could get away with a pf jar microwaved.. which im also gonna try and prove as soon as i pick up some verm.. the pf tek can be performed at 212'f (100'c) and the water doesnt get any hotter than that at normal atmospheric pressure.. which is why we pressure cook.. id think the water thats boiling would be far cleaner than the jars contents right? i understand the concept of steam and all but the water would be the most steamed part of the mix really.. and closest to the heat source on the stove which is hotter than boiling.. also the microwave is known to superheat water in some instances exploding in the users face! this happens with smooth containers and clean water.. if you can see it boiling in the microwave your safe.. if its just chillin and not boiling and you know it should be.. its probably alot hotter than boiling point.. you should let it cool inside the MW so you dont get blown up on however in this instance it would be a favorable condition.. am i somewhat correct or what?
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: wow]
#14085678 - 03/08/11 08:42 AM (12 years, 10 months ago) |
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Earth Child


Registered: 12/16/10
Posts: 3,473
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Re: Using a microwave for sterilization? [Re: wow]
#14267881 - 04/10/11 04:43 PM (12 years, 9 months ago) |
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So, you shouldn't use a microwave to sterilize cakes in a jar, as opposed to boiling? (Sorry to reopen old thread, but I was actually going to post something pretty similar, so just thought this might be better)
-------------------- Life is too short for this sorrow. You may be here today and gone tomorrow.
My Poetry · Stay Informed · Recipes · Nature Wants Us to Trip
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SomeGuy
I feel better now :)


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Re: Using a microwave for sterilization? [Re: Earth Child]
#14267914 - 04/10/11 04:50 PM (12 years, 9 months ago) |
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I sterilize al of my grows on a campfire I'm just kidding
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3Cajun1Mo8
Fung Padawan



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Re: Using a microwave for sterilization? [Re: Earth Child]
#14269133 - 04/10/11 08:48 PM (12 years, 9 months ago) |
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Quote:
Psybe said: So, you shouldn't use a microwave to sterilize cakes in a jar, as opposed to boiling? (Sorry to reopen old thread, but I was actually going to post something pretty similar, so just thought this might be better)
If your gonna do cakes either steam in a boiling pot of water or pressure cook them.
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mycofever
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Re: Using a microwave for sterilization? [Re: wow]
#17534847 - 01/11/13 08:59 PM (11 years, 20 days ago) |
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I know that this thread is 675 days old but I must add to the information in here that... At standard pressure, water boils at 212 degrees F (Or 100 Celsius). Above 212, water becomes steam- and may be MUCH hotter than 212. But boiling water will never be hotter than 212. Having said that your sterilization should be done elevated out of the water. which is not logical in a microwave.
-------------------- Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.
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PussyFart
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Re: Using a microwave for sterilization? [Re: mycofever]
#17536103 - 01/12/13 03:00 AM (11 years, 20 days ago) |
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Microwave ovens do not sterilize.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Mr Newbitall
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Re: Using a microwave for sterilization? [Re: velvetmagician]
#17536137 - 01/12/13 03:24 AM (11 years, 20 days ago) |
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although im new i do know that they use microwaves to sterilize food products im just not sure if a normal everyday microwave would work
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Kizzle
Misanthrope


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Re: Using a microwave for sterilization? [Re: mycofever]
#17536145 - 01/12/13 03:30 AM (11 years, 20 days ago) |
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Quote:
Above 212, water becomes steam- and may be MUCH hotter than 212.
Nah, the steam won't rise above 212F either without increasing the pressure and that can only be done with a PC.
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: Kizzle]
#17539895 - 01/12/13 09:42 PM (11 years, 19 days ago) |
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i laugh everytime 
http://www.shroomery.org/forums/showflat.php/Number/14049567/fpart/1/vc/1
microwave lc's with nothing more than 600ml tap water and <10ml karo corn syrup in the microwave for 5 minutes twice works awesome too!
a veteran grower dissed on a MW a long time ago being cool and now dissing the microwave is the cool thing to do!
i am absolutely sure i can duplicate this again! also.. keep in mind i went from spore to spore here using nothing more than a damn microwave!!
it depends what your doing.. but it works if done to the right things the right way obviously in my experience!
MICROWAVE FTW!!
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: Kizzle]
#17539952 - 01/12/13 09:55 PM (11 years, 19 days ago) |
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Quote:
Kizzle said:
Quote:
Above 212, water becomes steam- and may be MUCH hotter than 212.
Nah, the steam won't rise above 212F either without increasing the pressure and that can only be done with a PC.
"It is not true that water can only get up to 212 degrees and as cold as 32 degrees. After water changes from a liquid to a gas (at 212 degrees Fahrenheit) it can actually heat up much hotter than that. In the gas form, water molecules are spread out and have a lot of room to move and get much hotter than the other two phases (liquid and ice). And water freezes at 32 degrees Fahrenheit. But it can actually get colder than that, all the way toward what we call absolute zero. This value is equivalent to about -459 degrees Fahrenheit. This is when the water molecules are basically not moving."
http://van.physics.illinois.edu/qa/listing.php?id=1799
in fact, steam can be up to 700'F hotter than boiling water!!
the issue i found was more of a problem was the fact that if you heat something for too long it dries out! if you get it just right something can be sterilized to a degree that myc can grow on it! in my case it didn't take long for the lc to colonize my small cake which allowed for full colonization in the short window before any contams germinated..
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gimp
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Re: Using a microwave for sterilization? [Re: wow]
#17540495 - 01/13/13 12:13 AM (11 years, 19 days ago) |
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I have kit that says to microwave the casing substrate to sterilize it, is that wrong ?
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cubes4cancer
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Re: Using a microwave for sterilization? [Re: gimp]
#17540515 - 01/13/13 12:17 AM (11 years, 19 days ago) |
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Quote:
gimp said: I have kit that says to microwave the casing substrate to sterilize it, is that wrong ?
you have a "kit"...that sir is wrong!!
-------------------- $$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
  ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc. In other words,it's hard to fuck up a cube grow. RR
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PussyFart
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Re: Using a microwave for sterilization? [Re: gimp]
#17540538 - 01/13/13 12:24 AM (11 years, 19 days ago) |
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Quote:
gimp said: I have kit that says to microwave the casing substrate to sterilize it, is that wrong ?
First of all there is no such thing as a casing substrate.
There is a casing layer, then there is a bulk substrate.
Yes that is wrong, microwave ovens do not sterilize.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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wow
Lurking master



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Re: Using a microwave for sterilization? [Re: PussyFart]
#17540668 - 01/13/13 01:15 AM (11 years, 19 days ago) |
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Quote:
Notahacker420 said:
Quote:
gimp said: I have kit that says to microwave the casing substrate to sterilize it, is that wrong ?
First of all there is no such thing as a casing substrate.
There is a casing layer, then there is a bulk substrate.
Yes that is wrong, microwave ovens do not sterilize. 
i don't know about the casing layer being microwaved... but sterilization in microwaves AGAIN! has been PROVEN!
http://news.ufl.edu/2007/01/22/zap-the-bugs/
its not exactly as dependable as a pressure cooker, however it does work! i have prepared brown rice spawn, lc's, and sterilized syringes successfully MANY MANY times in the MICROWAVE! THAAAAAA MIIIIICCRROOOWAAAVE!! 
AGAIN check out one of my microwave experiments!!
http://www.shroomery.org/forums/showflat.php/Number/14049567/fpart/1/vc/1
Edited by wow (01/13/13 01:19 AM)
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PussyFart
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Re: Using a microwave for sterilization? [Re: wow]
#17540705 - 01/13/13 01:58 AM (11 years, 19 days ago) |
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Quote:
RogerRabbit said: I find it odd that only dumb stoners appear understand the difference between a cooked loaf of bread having the mold spores on its surface whacked, and wet grains with bacteria and/or bacterial endospores throughout the entire jar(where microwaves don't penetrate). It's two separate things. Mold spores are killed by temperature far below boiling. Bacteria needs 20 minutes at or above 100C, and you can't heat water above 100C in an unsealed vessel.
Naysayers have not held microwave sterilization back. Repeated failures and the laws of physics have. RR
http://www.shroomery.org/forums/showflat.php/Number/17330892#17330892
This is why we still have autoclaves beside the microwave oven (which has many other uses) in every microbiology lab. The best reason is that to really kill everything you need to heat the stuff around 120°C for 15 to 20 minutes. And even though some of the toughest spores may survive (rare, but a pain when it happens and you have to throw away liters of culture medium ). In the other hand your microwave will never heat anything over 100°C. Plus you may damage your microwave if there isn't enough water or anything to absorb the microwaves inside the oven. Definitely not a good thing to do.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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RogerRabbit
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Re: Using a microwave for sterilization? [Re: velvetmagician]
#17541143 - 01/13/13 01:58 AM (11 years, 19 days ago) |
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This thread has been closed.
Reason: This thread needs to die a horrible death before it steers even more noobs the wrong way. The members who boldly post ridiculous nonsense due to a total lack of basic 5th grade science education around here is embarrassing, quite frankly.
We can do better. RR
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