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OfflineCocaCody
Aspiring Mycologist
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Registered: 03/07/11
Posts: 7
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Last seen: 12 years, 10 months
Injection Port
    #14084128 - 03/07/11 10:28 PM (12 years, 10 months ago)

I ordered a bag from Mycofactory and to my dissatisfaction found there to be no injection port even though they are all supposed to come with one.

Anyways I don't want to pay any extra shipping fees, they already pretty much ripped me off on the shipping to get it to me as it cost more than the bag itself. This is my first time growing and I need to know how to make my own port so that I won't risk contamination.

I was reading and seen suggestions of silicone, does that really work? If so how long do I let it set up and do I need to take any other measures after I inoculate the bag through the silicone? If this won't work I'm open for other suggestions I just need an easy way to inoculate this bag.


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I plan on doing some good for the world with the help of mycology!


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InvisibleCH HELL
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Re: Injection Port [Re: CocaCody]
    #14084259 - 03/07/11 11:04 PM (12 years, 10 months ago)

Just use duct tape.  Clean the bag with alcohol the put duct tape over that spot,  inject through the duct tape and put another piece over the hole.
CH


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OfflinePsilocyentist
Carbon based


Registered: 11/04/10
Posts: 858
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Last seen: 5 years, 6 months
Re: Injection Port [Re: CH HELL]
    #14084299 - 03/07/11 11:14 PM (12 years, 10 months ago)

Or swirl some silicone and inject through that, self healing.


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I know, I know you probably scream and cry

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Invisiblebiologys
Mycologist in Trainning
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Re: Injection Port [Re: Psilocyentist]
    #14084321 - 03/07/11 11:19 PM (12 years, 10 months ago)

silicone / rtv will peel off most plastic surfaces unless ya take peice of sandpaper and ruff it up a bit..


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OfflineCocaCody
Aspiring Mycologist
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Registered: 03/07/11
Posts: 7
Loc: United States Flag
Last seen: 12 years, 10 months
Re: Injection Port [Re: biologys]
    #14084495 - 03/08/11 12:07 AM (12 years, 10 months ago)

Yeah I wasn't sure if silicone would stick to the bags (not that I've ever even used the stuff before)

If I use duct tape the first layer I lay down I should probably wipe down with alcohol, or would that ruin the tape?


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I plan on doing some good for the world with the help of mycology!


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OfflinePsilocyentist
Carbon based


Registered: 11/04/10
Posts: 858
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Last seen: 5 years, 6 months
Re: Injection Port [Re: biologys]
    #14084503 - 03/08/11 12:09 AM (12 years, 10 months ago)

Quote:

biologys said:
silicone / rtv will peel off most plastic surfaces unless ya take peice of sandpaper and ruff it up a bit..



damn, o well, I could have sworn I've seen it in someones bag tek... There's also these


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I know, I know you probably scream and cry

That your little world won't let you go



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InvisibleDoc_T
Random Dude
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Re: Injection Port [Re: CH HELL]
    #14084555 - 03/08/11 12:23 AM (12 years, 10 months ago)

Quote:

CH HELL said:
Just use duct tape.  Clean the bag with alcohol the put duct tape over that spot,  inject through the duct tape and put another piece over the hole.
CH




I bet you could use plain scotch tape in a pinch. It's just as sterile, though not as flexible.


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InvisibleCH HELL
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Re: Injection Port [Re: CocaCody]
    #14084648 - 03/08/11 12:56 AM (12 years, 10 months ago)

Quote:

CocaCody said:
Yeah I wasn't sure if silicone would stick to the bags (not that I've ever even used the stuff before)

If I use duct tape the first layer I lay down I should probably wipe down with alcohol, or would that ruin the tape?




Just wipe the bag with alcohol where you are going to inject.Get your duct tape and tear the first inch or two off and toss it,  the adhesive side should be sterile now place the tape where you wiped.  The tape should adhere nicely if it doesn't your alcohol didn't evaporate enough.  Once the tape is on the bag you can inject through it quickly and place another piece over the hole.
Or use a flow hood or GB and open the bag and squirt your spores in.

CH


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Offline3n1gm4
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Re: Injection Port [Re: CH HELL]
    #14084850 - 03/08/11 01:56 AM (12 years, 10 months ago)

You could always use the sticky part of a bandaid after cleaning the inoc point, they are already sterile, then just put another sticky part on top of hole.(tear sticky off with clean hands)Don't get it wet though, you know how those things are always slipping off when someone is tossing a salad...


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OfflineCocaCody
Aspiring Mycologist
Male


Registered: 03/07/11
Posts: 7
Loc: United States Flag
Last seen: 12 years, 10 months
Re: Injection Port [Re: 3n1gm4]
    #14085751 - 03/08/11 09:10 AM (12 years, 10 months ago)

Alright thanks everyone for your input I'm going to inoculate this bag today, probably with duct tape. In the future I'm just going to buy some injector ports in case this ever happens again -.-


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I plan on doing some good for the world with the help of mycology!


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