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OfflineMidlosouthside
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Registered: 02/10/11
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Pf block tek problems *DELETED*
    #14083127 - 03/07/11 07:13 PM (12 years, 11 months ago)

Post deleted by Midlosouthside

Reason for deletion: Noice


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Invisiblesandman420
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Re: Pf block tek problems [Re: Midlosouthside]
    #14083602 - 03/07/11 08:49 PM (12 years, 11 months ago)

all 18 cups in one bag or what?


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Offlineesquaredx
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Re: Pf block tek problems [Re: sandman420]
    #14083645 - 03/07/11 08:55 PM (12 years, 11 months ago)

What temps is it kept at?
How was your liquid culture made?

I would just wait a few days. I made liquid cultures that
took a little bit to show growth for some reason ( bit faster then spores tho).
Just wait and see :shrug:
Get ready to make more bags just incase.


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OfflineMidlosouthside
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Re: Pf block tek problems [Re: sandman420]
    #14083728 - 03/07/11 09:09 PM (12 years, 11 months ago)

Yes all 18 cups went in one bag per the tek

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OfflineMidlosouthside
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Re: Pf block tek problems [Re: esquaredx]
    #14083748 - 03/07/11 09:12 PM (12 years, 11 months ago)

The bag is stored at 75 degrees and my lc was made per the baby bottle tek which i tested on a regular pf cake and it was fine

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OfflineMidlosouthside
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Re: Pf block tek problems [Re: Midlosouthside]
    #14083760 - 03/07/11 09:13 PM (12 years, 11 months ago)

Could it be the bob red mills 10 grain bread mix? I thought it would be fine but one of the ingriedients is salt. Maybe thats why?

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Offlineesquaredx
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Re: Pf block tek problems [Re: Midlosouthside]
    #14083767 - 03/07/11 09:14 PM (12 years, 11 months ago)

I would just let the bag chill and hope it does its thing soon.
In the mean time, go get more supplies and make a few bags this time.
Making just one sucks, if it fails it fails.


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InvisibleJavadog
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Re: Pf block tek problems [Re: Midlosouthside]
    #14083844 - 03/07/11 09:33 PM (12 years, 11 months ago)

"no water came out but it held its form"

OK, your substrate might have been a bit dry, but as far as initial colonization
goes this is not usually a problem. (I find that growth can stall from dryness though)

Wait it out, and take notes for the next attempt.

Good luck!

JD


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Invisiblehamloaf
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Re: Pf block tek problems [Re: Midlosouthside]
    #14090985 - 03/09/11 05:33 AM (12 years, 11 months ago)

Quote:

after the water was added seemed fine i grabbed a handfull and squeezed and no water came out but it held its form.



No, Field Capacity is a few drops to a small stream or rivulet of water coming out of the material when you squeeze the substrate in your hand.

7 days is too long for LC to not show signs of recovery.  75F is a high colonization temperature, as well as, 15psi for an hour and a half, too short of a sterilization cycle for a substrate bag that large.  Use agar instead of LC or at least use light malt extract as the nutrient source for your LC.  Colonize substrate bags larger than 3 quarts @ 70F or below.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineMidlosouthside
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Re: Pf block tek problems [Re: hamloaf]
    #14101305 - 03/10/11 10:28 PM (12 years, 11 months ago)

So how long would i need to pc the bags?

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Invisiblehamloaf
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Re: Pf block tek problems [Re: Midlosouthside]
    #14102414 - 03/11/11 04:23 AM (12 years, 11 months ago)

Depends on what psi your PC can reach.  If you can reach 20 psi's, I'd PC for 2 hours at 20 psi's. 

If all your PC'll reach is 15 psi, best bet on a substrate bag that large and dense would be to PC for 3 hours at 15 psi's to be sure the internal core of the substrate reaches and holds the proper temperature for the correct amount of time.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

Edited by liquidmyce (03/11/11 04:46 AM)

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