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OfflineAjaxx
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First LC - A couple questions
    #14082198 - 03/07/11 04:35 PM (12 years, 10 months ago)

getting ready to attempt my first LC. on hand i have karo, pure dextrose, and 2 cc of PF albino, not to mention a handful of jars.

question 1


if i use dextrose, am i also suppose to combine it with malt, or can i just use it alone?

question 2


since i have 2cc's of spore solution, should i use all of it for 1 jar of LC, or can i make 2 LCs with 1 cc per jar?


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Offlinecuriousgeorge91
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Re: First LC - A couple questions [Re: Ajaxx]
    #14082239 - 03/07/11 04:42 PM (12 years, 10 months ago)

For your first question, im not totally sure, but i think you need just one of them.
As for your second question, you can easily make 2 lc's with 2cc, you could even make four if u wanted.  More spores sometimes gives u faster growth, but less means less genetic variability. And less waste for that matter.  And given the likelihood of LC's failing, i would make at least 2 to increase your chances of success:shrug:

thats my 2 cents


--------------------
The shoe that fits one person pinches another; there is no recipe for living that suits all cases.

-  Carl Jung


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OfflineAjaxx
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Re: First LC - A couple questions [Re: curiousgeorge91]
    #14082257 - 03/07/11 04:45 PM (12 years, 10 months ago)

likelihood of LCs failing?

once i shoot the LC jars up, do i swirl them around a little bit to mix the spores throughout the solution?



Edited by Ajaxx (03/07/11 04:51 PM)


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Offlineesquaredx
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Re: First LC - A couple questions [Re: Ajaxx]
    #14082285 - 03/07/11 04:50 PM (12 years, 10 months ago)

Quote:

Ajaxx said:
likelihood of LCs failing?




Cant really say. But for someone who has never made them,
Hope for the best and expect the worse.
Goodluck.

What did you use the rest of your spores on?
rye, wbs, brf?


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Offlinecuriousgeorge91
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Re: First LC - A couple questions [Re: Ajaxx]
    #14082292 - 03/07/11 04:51 PM (12 years, 10 months ago)

Ive never personally tried one, i really want to because i like the sounds of them in theory, but most ppl say that they are very prone to failure. I guess its because u cant spot contams...?
Just follow your tek and test them on a brf cake or w.e your using. Basically, just dont go ahead and knock up a hundred jars with it before you know if its clean:thumbup:.
Good Luck


--------------------
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-  Carl Jung


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Offlineesquaredx
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Re: First LC - A couple questions [Re: curiousgeorge91]
    #14082301 - 03/07/11 04:53 PM (12 years, 10 months ago)

Yeah you cant tell if its contamed untill you shoot up a test jar.
Id make some myc water, that shit powns. :thumbup:


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OfflineAjaxx
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Re: First LC - A couple questions [Re: esquaredx]
    #14082302 - 03/07/11 04:53 PM (12 years, 10 months ago)

Quote:

esquaredx said:
Quote:

Ajaxx said:
likelihood of LCs failing?




Cant really say. But for someone who has never made them,
Hope for the best and expect the worse.
Goodluck.

What did you use the rest of your spores on?
rye, wbs, brf?




the spores are left over from my first (successful) grow from a year ago or so. i grew on BRF cakes and then cased.

im planning on going with rye berries this time, to monotubs.


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OfflineAjaxx
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Re: First LC - A couple questions [Re: esquaredx]
    #14082303 - 03/07/11 04:54 PM (12 years, 10 months ago)

Quote:

esquaredx said:
Yeah you cant tell if its contamed untill you shoot up a test jar.
Id make some myc water, that shit powns. :thumbup:




myc water?


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Offlinemushroomhunter10
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Re: First LC - A couple questions [Re: curiousgeorge91]
    #14082305 - 03/07/11 04:55 PM (12 years, 10 months ago)

I would make more than 2 jars, and also with LC's you can't tell if it's a contaminate or mycelium. So more jars is better just in case some end up being contaminants.

The other dude is right. You should try 4 jars. If your syringe is "thick" with lots of visible spores then you have more than enough for 4.


--------------------
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OfflineAjaxx
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Re: First LC - A couple questions [Re: mushroomhunter10]
    #14082330 - 03/07/11 05:00 PM (12 years, 10 months ago)

Quote:

mushroomhunter10 said:
I would make more than 2 jars, and also with LC's you can't tell if it's a contaminate or mycelium. So more jars is better just in case some end up being contaminants.

The other dude is right. You should try 4 jars. If your syringe is "thick" with lots of visible spores then you have more than enough for 4.




bad thing about albino mushrooms is that the spores are clear...

ill go ahead and make 4 LCs and cross my fingers.


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Offlineesquaredx
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Re: First LC - A couple questions [Re: Ajaxx]
    #14082334 - 03/07/11 05:00 PM (12 years, 10 months ago)

Quote:

Ajaxx said:
Quote:

esquaredx said:
Yeah you cant tell if its contamed untill you shoot up a test jar.
Id make some myc water, that shit powns. :thumbup:




myc water?




Grain LC.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0

Way better in my book.


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Offlinecuriousgeorge91
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Re: First LC - A couple questions [Re: esquaredx]
    #14082365 - 03/07/11 05:06 PM (12 years, 10 months ago)

Isn't myc water LC??? pretty sure. Lets not cause necessary confusion here come on lol:stoned:


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Offlineesquaredx
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Re: First LC - A couple questions [Re: curiousgeorge91]
    #14082376 - 03/07/11 05:08 PM (12 years, 10 months ago)

Im pretty sure they are all different types of LC's.
The better thing about myc water, is you already know its not contamed if your jar is good. :shrug:
When I hear lc I always think of people using karo or honey. Ewww.


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Invisiblebiologys
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Re: First LC - A couple questions [Re: curiousgeorge91]
    #14082379 - 03/07/11 05:08 PM (12 years, 10 months ago)

Myc water is GLC pretty much.


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Offlineesquaredx
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Re: First LC - A couple questions [Re: biologys]
    #14082395 - 03/07/11 05:11 PM (12 years, 10 months ago)

I was just recommending he make some myc water, I think its easier to do then a regular honey or karo lc. Less failure rate.
Go ahead and make your LC, shoot up a jar of grains, and turn that into some myc water. Wont be mad at all. :thumbup:
Goodluck. Let us know how it goes.


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Re: First LC - A couple questions [Re: esquaredx]
    #14082630 - 03/07/11 06:00 PM (12 years, 10 months ago)

Mycelium water begins with grains. LC begins with all liquid, or solids that dissolve into liquid. Either way it ends up as a liquid.

Grain LC all the way. I will never ever do liquid LC once I saw the power of Grain LC. :smile:


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES


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InvisibleStains Blue
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Re: First LC - A couple questions [Re: Ajaxx]
    #14082756 - 03/07/11 06:16 PM (12 years, 10 months ago)

Quote:

Ajaxx said:
question 1

if i use dextrose, am i also suppose to combine it with malt, or can i just use it alone?


Never tried it.


Quote:

Ajaxx said:
question 2

since i have 2cc's of spore solution, should i use all of it for 1 jar of LC, or can i make 2 LCs with 1 cc per jar?




I have made LC's with as little as 2 drops of a spore syringe.

And I don't swirl mine at first. I wait 4 or 5 days when I see the first signs on mycelium.
Then I swirl them. Or put them on my magnetic stir plate.


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OfflineAjaxx
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Re: First LC - A couple questions [Re: esquaredx]
    #14083788 - 03/07/11 09:21 PM (12 years, 10 months ago)

Quote:

esquaredx said:
Quote:

Ajaxx said:
Quote:

esquaredx said:
Yeah you cant tell if its contamed untill you shoot up a test jar.
Id make some myc water, that shit powns. :thumbup:




myc water?




Grain LC.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0

Way better in my book.




well since im a new member i cant view that page. i talked to a friend of mine earlier who grows edibles on and off, and he explained it to me, and now it seems pointless to even do an LC now.

so say i have a fully colonized jar, and i put X amount of sterile water in it, mix up, then pull the water back out, when drawn up in a syringe, how much will it take to inoculate a new jar? a couple drops or 1 cc?

and

when i extract all of the mycelium water, can i still use that colonized substrate to spawn to a monotub? and if i can, does it still colonize at the same speed, or does it slow it down at all from taking a bunch of mycelium away from it?


Edited by Ajaxx (03/07/11 09:23 PM)


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Offlineesquaredx
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Re: First LC - A couple questions [Re: Ajaxx]
    #14083799 - 03/07/11 09:24 PM (12 years, 10 months ago)

You dont need alot of myc water at all for a jar.
That shit grows like wild fire! haha.
And the jar you used to make the myc water can be grown with.
Just need to watch it since there may be excess water in the jar after doing this.


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OfflineAjaxx
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Re: First LC - A couple questions [Re: esquaredx]
    #14083838 - 03/07/11 09:32 PM (12 years, 10 months ago)

sweet. thanks for all the help guys :smile: i cant wait to get this project started!


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