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OfflineD Dawg
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first mono tub contaminated
    #14076730 - 03/06/11 05:32 PM (12 years, 11 months ago)

so my first tub was a learning experience. I used WBS and spawned to coir. however the tub seemed to be colonizing nicely and then contaminated with trich before it was done.

what could have caused this?

i have read that contaminated spawn is usually to blame when this happens but i was fairly certain the spawn was fine as it was a nice white color and smelt good. I did however use too much water in the coir and also left the entire tub in a garbage bag to colonize which i now know is the incorrect thing to do. is it possible these factors gave me the trich contamination or is it for certain contaminated spawn?

thanks for any help.

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InvisibleDoc_T
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Re: first mono tub contaminated [Re: D Dawg]
    #14076737 - 03/06/11 05:33 PM (12 years, 11 months ago)

Plain coir? Or what? How did you prep it?


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Invisiblepenhed
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Re: first mono tub contaminated [Re: Doc_T]
    #14076813 - 03/06/11 05:46 PM (12 years, 11 months ago)

to wet+ a bag =:sad:

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OfflineSmirnoff
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Re: first mono tub contaminated [Re: penhed]
    #14076864 - 03/06/11 05:55 PM (12 years, 11 months ago)

Quote:

penhed said:
to wet+ a bag =:sad:




yea I've found with time that too dry is better than too wet....


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Re: first mono tub contaminated [Re: Smirnoff]
    #14076893 - 03/06/11 05:59 PM (12 years, 11 months ago)

Too wet is bad- but the contam doesn't come from water. Bad spawn is the most likely source.


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OfflineD Dawg
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Re: first mono tub contaminated [Re: Doc_T]
    #14077040 - 03/06/11 06:26 PM (12 years, 11 months ago)

yes just plain coir, and i only prepped it by hydrating it with boiling water. i also added a light casing layer on top. sounds like i should pasteurize and go a little drier with the coir next time.

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Re: first mono tub contaminated [Re: D Dawg]
    #14077050 - 03/06/11 06:27 PM (12 years, 11 months ago)

Light casing layer of... what? Prepped how?


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OfflineD Dawg
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Re: first mono tub contaminated [Re: Doc_T]
    #14077065 - 03/06/11 06:30 PM (12 years, 11 months ago)

i just cased with more coir on top. and all i did to prep any of this was pour boiling water into a bucket containing the coir. i have seen this work for others

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Re: first mono tub contaminated [Re: D Dawg]
    #14077072 - 03/06/11 06:32 PM (12 years, 11 months ago)

And the trich- where did it show up? On top? All over? Just a spot? Massive?

How'd you prep your grain jars, btw? And how did you inoculate?


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OfflineD Dawg
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Re: first mono tub contaminated [Re: Doc_T]
    #14077096 - 03/06/11 06:36 PM (12 years, 11 months ago)

i was able to identify the trich at around day 6 (it looked too white to be mush), and it appeared in a single spot on one side of the tub. The grain was pressure cooked for maybe and hour and ten minutes and drained. i inoculated with a MS syringe through a hole that was taped after wards with tap that had already been in place through the pressure cooking. It did take about a month to colonize but i figured this was normal as i have always had slow colonization times here.

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Offlinei GrOw StUFF
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Re: first mono tub contaminated [Re: Doc_T]
    #14077105 - 03/06/11 06:37 PM (12 years, 11 months ago)

Im guessing that due to being in a bag it didn't get enough GE...When I use bags I make sure to open the bag for 1 second and then close it back up once a day....The tub has to be able to breath somewhat....


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Re: first mono tub contaminated [Re: D Dawg]
    #14077113 - 03/06/11 06:38 PM (12 years, 11 months ago)

PC'd for 70 minutes then drained? what do you mean by that statement...


also PC at 90 mins at 15 PSI

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OfflineD Dawg
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Re: first mono tub contaminated [Re: biologys]
    #14077119 - 03/06/11 06:39 PM (12 years, 11 months ago)

sorry i ment soaked drained and pressure cooked.

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Re: first mono tub contaminated [Re: i GrOw StUFF]
    #14077128 - 03/06/11 06:41 PM (12 years, 11 months ago)

Bad spawn. There's a small subtle contam in your syringe.

Yeah, wet and stifled is bad.
But the trich has to come from somewhere.
If the coir is clean and the spawn is clean, then there can be no contamination.

But that's if it's trich- you didn't wait to see green? Just tossed it?


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Offlinei GrOw StUFF
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Re: first mono tub contaminated [Re: biologys]
    #14077130 - 03/06/11 06:41 PM (12 years, 11 months ago)

Pasteurizing should be for 90 minutes.  Use  RR's tek for pasteurzing in jars....


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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OfflineD Dawg
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Re: first mono tub contaminated [Re: Doc_T]
    #14077174 - 03/06/11 06:46 PM (12 years, 11 months ago)

i didnt want to let it get green but im sure it was trich


thats what i had when i threw it out

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Offlinei GrOw StUFF
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Re: first mono tub contaminated [Re: D Dawg]
    #14077198 - 03/06/11 06:51 PM (12 years, 11 months ago)

yup, trich...In my opinion I think it was your pasteurization technique.  Did you seal it in a bag?  Was the bag able to breath?


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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Re: first mono tub contaminated [Re: i GrOw StUFF]
    #14077200 - 03/06/11 06:52 PM (12 years, 11 months ago)

and was the tub able to breath?


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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OfflineD Dawg
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Re: first mono tub contaminated [Re: i GrOw StUFF]
    #14077212 - 03/06/11 06:54 PM (12 years, 11 months ago)

no, i think sealing it in the plastic bag pretty much eliminated the GE unfortunately. the only hole in the tub would have been the gap between the lid and tub

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Offlinei GrOw StUFF
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Re: first mono tub contaminated [Re: D Dawg]
    #14077227 - 03/06/11 06:58 PM (12 years, 11 months ago)

So there is the problem.  Alot of times people use contaminated grains but in your case I'm pretty sure it was sealing the tub....Next time you should use polyfill or micropore tape for a couple holes on the tub itself to allow for GE.  If you use a bag make sure to air out the bag once a day only for a few seconds...


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

Edited by i GrOw StUFF (03/06/11 06:58 PM)

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