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Offlinehippyhater
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Registered: 02/25/11
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Last seen: 12 years, 11 months
Is it safe to inject liquid culture into pasteurized hpoo?
    #14076619 - 03/06/11 05:12 PM (12 years, 11 months ago)

The title pretty much says it all. If it is indeed safe, whats the best technique? Any help would be appreciated thanks.

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InvisibleDoc_T
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Re: Is it safe to inject liquid culture into pasteurized hpoo? [Re: hippyhater]
    #14076650 - 03/06/11 05:17 PM (12 years, 11 months ago)

Safe like will it explode?
Or safe like will it work to grow mushrooms?


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You make it all possible. Doesn't it feel good?

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Invisiblepenhed
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Re: Is it safe to inject liquid culture into pasteurized hpoo? [Re: Doc_T]
    #14076720 - 03/06/11 05:30 PM (12 years, 11 months ago)

:laugh:

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OfflineThe_Outsider
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Re: Is it safe to inject liquid culture into pasteurized hpoo? [Re: penhed]
    #14076729 - 03/06/11 05:31 PM (12 years, 11 months ago)

I have someone very strong spot you on this one for added saftey


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Offlinear1es
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Re: Is it safe to inject liquid culture into pasteurized hpoo? [Re: The_Outsider]
    #14078814 - 03/06/11 11:25 PM (12 years, 11 months ago)

inject into grains


wait until they are 100%

then mix with poooooo


but if you want to inject it right away its safe to do u just wont get any growth


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":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita

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Offlineshroomie_glen
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Re: Is it safe to inject liquid culture into pasteurized hpoo? [Re: ar1es]
    #14078976 - 03/07/11 12:00 AM (12 years, 11 months ago)

Here is the problem with what you have posed OP:
If you Inject an Lc DIRECTLY into any bulk substrate, it has a good chance of contamming for a couple reasons.

1.:There arent many inoculation points on a strand of liquid, so IF it even does begin to colonize, it will do so slooooooooowly.

2. Lc's have a tendency to contam even when perfect procedure is followed.  While other people have a lot of success witht hem, i see no need to even use them after moving to invitro petri work.  I guess people use Lcs because they are supposedly easier/simpler, but theyre not ideal for cubies just IMO.

Yes as someone aforementioned, inoculate grain, then use that to spawn to your bulk substrate.


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No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

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