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OfflineEastSidePsilocybe
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easiest lc lid? please help
    #14074465 - 03/06/11 07:15 AM (12 years, 10 months ago)

i want to start my first liquid culture very soon and would like to know the easiest way to make a lid that will suffice for an lc. ive gotten extremely frustrated at the lack of explanation of what certain pieces/products are and where i can get them. i would like to avoid buying anything online, unless i can get it at a


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Offlinek00laid
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Re: easiest lc lid? please help [Re: EastSidePsilocybe]
    #14074470 - 03/06/11 07:17 AM (12 years, 10 months ago)

take one lid.

drill or poke or stab or jab 2 holes.

stuff 1 hole with polyfil

stuff the other hole with silicone, RTV or otherwise.

let silicone dry/cure overnight. bam.

when tilting to get LC out be sure not to tilt on the polyfil side :3

but i am a proponent of GRAIN LIQUID CULTURES as opposed to sugar based.

you can find it on the search engine


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InvisibleDoc_T
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Re: easiest lc lid? please help [Re: k00laid]
    #14074494 - 03/06/11 07:30 AM (12 years, 10 months ago)

^^^ completely agree.


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InvisibleMorelman
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Re: easiest lc lid? please help *DELETED* [Re: EastSidePsilocybe]
    #14074545 - 03/06/11 07:58 AM (12 years, 10 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Invisiblevirus1824
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Re: easiest lc lid? please help [Re: Morelman]
    #14075043 - 03/06/11 10:43 AM (12 years, 10 months ago)

i do this with gasoline tubing and polyfil. Also works against getting wet poly



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InvisibleTranscendingLife
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Re: easiest lc lid? please help [Re: virus1824]
    #14075085 - 03/06/11 10:57 AM (12 years, 10 months ago)

I fucked w/ Morelman's no-tilt design:



These work just fine too, my standard grain lids:


You're making it way to difficult on yourself. A lid is a lid. All you need is an Inoculation point + a GE hole covered w/ some type of filter media...


--------------------
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InvisibleFrankHorrigan
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Re: easiest lc lid? please help [Re: TranscendingLife]
    #14075109 - 03/06/11 11:06 AM (12 years, 10 months ago)

i use a 1/4" hole in a mason jar lid covered with micropore tape and a self-healing port (http://www.labwarehouse.com/lw/category.cfm?category=50 item# 28991) on the other side for the needle.


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InvisibleTranscendingLife
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Re: easiest lc lid? please help [Re: FrankHorrigan]
    #14075148 - 03/06/11 11:16 AM (12 years, 10 months ago)

Quote:

yakwhacker said:
i use a 1/4" hole in a mason jar lid covered with micropore tape and a self-healing port (http://www.labwarehouse.com/lw/category.cfm?category=50 item# 28991) on the other side for the needle.




RTV silicone makes a self-healing port. Those self-healing injection ports are a waste of money IMHO. I bought 100 of the bitches & threw out all 3 that I experimented w/. They get used up real quick & then you have to fill then w/ RTV.

Skip the self-healing injection ports & just use RTV for injection ports :2cents:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleMorelman
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Re: easiest lc lid? please help *DELETED* [Re: TranscendingLife]
    #14075168 - 03/06/11 11:22 AM (12 years, 10 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Edited by Morelman (03/06/11 11:34 AM)


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InvisibleTranscendingLife
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Re: easiest lc lid? please help [Re: Morelman]
    #14075188 - 03/06/11 11:27 AM (12 years, 10 months ago)

yea, RTV does wear out as well, but It's a lot easier & cheaper than buying injection ports.

Besides, You should silicone in your injection ports too, so why not just save yourself the hassle?


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisiblevirus1824
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Re: easiest lc lid? please help [Re: TranscendingLife]
    #14075197 - 03/06/11 11:29 AM (12 years, 10 months ago)

Also he said the easiest, not which is the best with parts found on ebay.


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OfflineEastSidePsilocybe
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Re: easiest lc lid? please help [Re: Morelman]
    #14075286 - 03/06/11 11:56 AM (12 years, 10 months ago)

Quote:

Morelman said:
Quote:

EastSidePsilocybe said:
i want to start my first liquid culture very soon and would like to know the easiest way to make a lid that will suffice for an lc. ive gotten extremely frustrated at the lack of explanation of what certain pieces/products are and where i can get them. i would like to avoid buying anything online, unless i can get it at a




Example?

What was described will suffice and it is easy and cheap.




i couldnt seem to find any of the equipment named on all of the teks on amazon..im about to order a bunch of shit from there and i dont want to have to make multiple orders from mycology websites. im still pretty new so im not looking to spend a bunch of money on making the most fool proof lc possible..just something budget and easy.


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InvisibleTranscendingLife
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Re: easiest lc lid? please help [Re: EastSidePsilocybe]
    #14075296 - 03/06/11 11:59 AM (12 years, 10 months ago)

Go to wally world.
Pick up RTV silicone & E-Z-Felt
make your holes. Punch or Drill
Fill one w/ RTV
Cover the other w/ the felt sealed on the exterior of the lid w/ silicone.
Let cure over night.
Done.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineEastSidePsilocybe
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Re: easiest lc lid? please help [Re: TranscendingLife]
    #14075324 - 03/06/11 12:07 PM (12 years, 10 months ago)

Appreciate all the responses, guys.


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InvisibleTranscendingLife
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Re: easiest lc lid? please help [Re: EastSidePsilocybe]
    #14075326 - 03/06/11 12:08 PM (12 years, 10 months ago)

Morelman :pm: sent :awethumb:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisiblevirus1824
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Re: easiest lc lid? please help [Re: TranscendingLife]
    #14075335 - 03/06/11 12:10 PM (12 years, 10 months ago)

I'm a diabetic myself and have never seen those bottles.


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InvisibleMorelman
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Re: easiest lc lid? please help *DELETED* [Re: virus1824]
    #14075424 - 03/06/11 12:31 PM (12 years, 10 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Invisiblevirus1824
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Re: easiest lc lid? please help [Re: Morelman]
    #14075454 - 03/06/11 12:38 PM (12 years, 10 months ago)

http://www.diabeteseducationblog.org/voices/2009/05/insulin-pens-or-syringes.html

I see in America vials are more common. Im from Europe and i guess you from USA i use this with a needle screwed on of course.



Btw sorry for the threadjack.


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Offlineshroomtastic89
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Re: easiest lc lid? please help [Re: virus1824]
    #14075759 - 03/06/11 01:58 PM (12 years, 10 months ago)

You think i could stuff the GE hole with a cotton ball?


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Invisiblebiologys
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Re: easiest lc lid? please help [Re: shroomtastic89]
    #14075764 - 03/06/11 01:59 PM (12 years, 10 months ago)

no dont use cotton..if you cant get polyfil or tyvek cut open an old stuffed animal, or pillow and use the polyfil from that..


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Offlineshroomtastic89
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Re: easiest lc lid? please help [Re: biologys]
    #14075771 - 03/06/11 02:01 PM (12 years, 10 months ago)

I can get polyfill at walmart correct? I'll prolly just go pick some up...


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Invisiblebiologys
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Re: easiest lc lid? please help [Re: shroomtastic89]
    #14075780 - 03/06/11 02:03 PM (12 years, 10 months ago)

yes as long as they have a arts and crafts section..most walmarts around here does.. you can get a big bag for like 4 bucks


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Offlineshroomtastic89
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Re: easiest lc lid? please help [Re: biologys]
    #14075806 - 03/06/11 02:10 PM (12 years, 10 months ago)

tits and your money will be in paypal tonight for those PE... Thanks again


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