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Offlinerandy420rhoads
Male

Registered: 02/24/07
Posts: 535
Last seen: 11 years, 6 months
Agar spots
    #14072266 - 03/05/11 06:39 PM (13 years, 11 hours ago)

This is on all my plates, are they trash?


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InvisiblePony
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Registered: 11/01/09
Posts: 287
Loc: Oklahoma
Re: Agar spots [Re: randy420rhoads]
    #14072272 - 03/05/11 06:40 PM (13 years, 11 hours ago)

it's a little blurry, did you put any spawn on it yet, or is that just unused prepared agar?


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InvisibleOzzy
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Posts: 1,067
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Re: Agar spots [Re: Pony]
    #14072278 - 03/05/11 06:41 PM (13 years, 11 hours ago)

That looks like some nasty bacteria. toss it

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OfflineRogerRabbitM
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Registered: 03/26/03
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OG Cultivator
Re: Agar spots [Re: Pony]
    #14072280 - 03/05/11 06:42 PM (13 years, 11 hours ago)

Bacteria colonies, most likely from your own breath.  You'll never get anything useful to grow on those.  Next time, follow sterile procedure.
RR


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Offlinerandy420rhoads
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Re: Agar spots [Re: RogerRabbit]
    #14072295 - 03/05/11 06:46 PM (13 years, 11 hours ago)

Why's it always my bad breath with you ? lol

They never left my glovebox untill I took out for the picture, and my glovebox is completely sealed.

Edited by randy420rhoads (03/05/11 06:46 PM)

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InvisibleOzzy
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Re: Agar spots [Re: randy420rhoads]
    #14072312 - 03/05/11 06:48 PM (13 years, 11 hours ago)

then your sleeve was no longer sterile when you poured.
I had it happen to me on a sleeve that came from a very old pharmacy, every plate triched out even when poured in front of my flowhood.

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Offlinerandy420rhoads
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Re: Agar spots [Re: Ozzy]
    #14072317 - 03/05/11 06:50 PM (13 years, 10 hours ago)

Hmm well I still have some unused agar in a quart jar is there anyway to restrilize it or do I need to order more?

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InvisibleOzzy
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Re: Agar spots [Re: randy420rhoads]
    #14072336 - 03/05/11 06:56 PM (13 years, 10 hours ago)

yup, should still be good. Go for it

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Offlinerandy420rhoads
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Registered: 02/24/07
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Re: Agar spots [Re: Ozzy]
    #14072344 - 03/05/11 06:58 PM (13 years, 10 hours ago)

Think i'm going to use 1/2pint jars just to insure a little more sterility so I know weather it was my fault or bad product.

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