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OfflineTerry M
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Registered: 06/18/10
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reserving some petri dishes from opened sleeve
    #14070682 - 03/05/11 12:20 PM (12 years, 10 months ago)

For some agar projects, I don't need a whole 25-plate sleeve of petri dishes. I'd like to just use 1/4 to 1/2 a sleeve to pour my agar. Is there any good way to keep the remainder in the opened sleeve sterile -- perhaps twist-locking the sleeve in the glove box, or quickly transfer to zip-lock bags and seal?

An alternative would be to pour all the dishes, reserve a portion of these, put in zip-lock bags and store in the frig for later use. This sounds iffy to me because of possible contaminants growing in the virgin agar dishes.

Anybody have any experience with this, or recommendations?


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Invisibleandymc
cocoa beetles from zanzibar
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Registered: 11/25/09
Posts: 2,395
Re: reserving some petri dishes from opened sleeve [Re: Terry M]
    #14070998 - 03/05/11 01:34 PM (12 years, 10 months ago)

I pour a couple of sleeves at a time and store in zip locs.  I go through 'em in 2-3 weeks usually.  Some dishes get bac, but I don't mind losing the odd one.

On occasions when I've used a partial sleeve, tying it up before removing from the glovebox seemed to work.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: reserving some petri dishes from opened sleeve [Re: andymc]
    #14071001 - 03/05/11 01:35 PM (12 years, 10 months ago)

Or, just take what you need and twist the sleeve up with what's left inside.
RR


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Offlinear1es
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Registered: 07/09/10
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Re: reserving some petri dishes from opened sleeve [Re: RogerRabbit]
    #14071149 - 03/05/11 02:19 PM (12 years, 10 months ago)

do you use parafilm to seal the petri dishes then place in zip locks and still get contams?


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