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InvisibleDynGBreeD
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Registered: 01/15/11
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Inoculation Jars. In Shower???
    #14070506 - 03/05/11 11:33 AM (12 years, 10 months ago)

So I was wondering. I have a stand up shower. The Door Goes from floor to ceiling. Im the only one who uses it. If it sterilize the shit out of it. Think i could sit in there And Inject my spores into My BRF jars. AND YES I KNOW WHAT THE TEK SAYS TO USE! Please dont post the Videos. Im simply just asking a Question for inquiring minds. Thanks Guys! And Gal Haha


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InvisibleTranscendingLife
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Re: Inoculation Jars. In Shower??? [Re: DynGBreeD]
    #14070527 - 03/05/11 11:38 AM (12 years, 10 months ago)

You're still inoculating jars in open air. How are you going to stop the contams that fall off of you in the shower?

The key thing to remember, is that we are the biggest carriers of contams that our mushrooms will be exposed to. I've read of people literally inoculating jars immediately after they got outta the shower, yes they were naked & still got contams. Why? Because they weren't covering themselves & inoculating BRF jars in open air...

Try it, but I doubt it will work :2cents:


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OfflineCynosure
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Re: Inoculation Jars. In Shower??? [Re: TranscendingLife]
    #14070570 - 03/05/11 11:49 AM (12 years, 10 months ago)

I know it wasn't the best of ideas, but my first two sets of jars (9 jars and 12 jars) were both inoculated in bathrooms that I cleaned the hell out of.  I wiped everything down with bleach.. let it air out for a day, returned with disinfectant, closed the door, disinfected it again, then inoculated.

I used a different bathroom each time and neither of my sets have contaminants. 

I say that you can probably get away with it as long as you clean the bathroom thoroughly.

I am going to be doing my next set, as well as syringe making, and LC construction in a proper glove-box.


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InvisibleDJYoshaBYD
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Re: Inoculation Jars. In Shower??? [Re: Cynosure]
    #14070601 - 03/05/11 12:00 PM (12 years, 10 months ago)

Quote:

Inject my spores into My BRF jars




yes, you totally can, and it will work.. if you are injecting them through a proper lid.. if its PF style jars, then it wont really be exposing the grow medium to the outside world..

granted, doing ANY sterile work inside of a glovebox will give you wayyyy more consistent results, but you dont NEED one.. especially not for PF jars.. you dont even have to open the lid.. just inject through the top.. obviously, be clean, and sterilize the needle first, but there is no reason you cannot just do it on the counter top in the bathroom, with no fan or ac or anything on.. no moving air..

i dont think you will have any issues if you prepped right..


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InvisibleDJYoshaBYD
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Re: Inoculation Jars. In Shower??? [Re: DJYoshaBYD]
    #14070608 - 03/05/11 12:01 PM (12 years, 10 months ago)

Quote:


I am going to be doing my next set, as well as syringe making, and LC construction in a proper glove-box.




giggity.. now THOSE are some good examples of glovebox use.. especially for making syringes..


Edited by DJYoshaBYD (03/05/11 12:01 PM)


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InvisibleDynGBreeD
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Re: Inoculation Jars. In Shower??? [Re: DJYoshaBYD]
    #14070639 - 03/05/11 12:09 PM (12 years, 10 months ago)

DJYoshaBYD These Are The Style Of Jars Im using.

Dumb ? BUT What does PF Stand For???


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InvisibleDJYoshaBYD
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Re: Inoculation Jars. In Shower??? [Re: DynGBreeD]
    #14070667 - 03/05/11 12:15 PM (12 years, 10 months ago)

PF = psylocybe fanaticus

ahh.. now i see what is meant by open air.. you can always make an inoculation port on those.. just a hole and some micropore tape..

if you have to open the lids to inoculate, then you will have better luck using even just a makeshift glovebox..


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OfflineCynosure
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Re: Inoculation Jars. In Shower??? [Re: DynGBreeD]
    #14070670 - 03/05/11 12:16 PM (12 years, 10 months ago)

Quote:

DJYoshaBYD said:
Quote:


I am going to be doing my next set, as well as syringe making, and LC construction in a proper glove-box.




giggity.. now THOSE are some good examples of glovebox use.. especially for making syringes..




=D

I hope it all works out fine... especially the LC.

Quote:

DynGBreeD said:
Dumb ? BUT What does PF Stand For???




Don't quote me on this, but I believe it stands for Psylocybe Fanaticus which is who (a team?) created the tek.


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InvisibleDynGBreeD
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Re: Inoculation Jars. In Shower??? [Re: DJYoshaBYD]
    #14070704 - 03/05/11 12:23 PM (12 years, 10 months ago)

Quote:

DJYoshaBYD said:
PF = psylocybe fanaticus

ahh.. now i see what is meant by open air.. you can always make an inoculation port on those.. just a hole and some micropore tape..

if you have to open the lids to inoculate, then you will have better luck using even just a makeshift glovebox..



I used a Dremel And Made 4 Holes Just Big Enough To fit the needle Through. So No Worries there. Thanks For all the help!


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InvisibleDJYoshaBYD
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Re: Inoculation Jars. In Shower??? [Re: Cynosure]
    #14070708 - 03/05/11 12:24 PM (12 years, 10 months ago)

correct.. they were a spore vendor, as well


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OfflineRogerRabbitM
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Re: Inoculation Jars. In Shower??? [Re: DJYoshaBYD]
    #14070991 - 03/05/11 01:33 PM (12 years, 10 months ago)

Showers are one of the nastiest places in any house.  Bacteria grows rampant on the soap film left on the door/curtain, and the drain is a breeding ground for some of the worst shit you'll ever see.  If you doubt this, make a slide and look at it through a microscope.

The two worst possible places in any house for inoculation are bathrooms and kitchens.
RR


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OfflinePatlal
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Re: Inoculation Jars. In Shower??? [Re: RogerRabbit]
    #14071080 - 03/05/11 01:58 PM (12 years, 10 months ago)

Ya...If I were to do that, I would scrub the tub with bleach...

Pour pure bleach down the drain...

But... RR is most than likely right...


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Offlineyessir
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Re: Inoculation Jars. In Shower??? [Re: Patlal]
    #14071229 - 03/05/11 02:36 PM (12 years, 10 months ago)

Well bathrooms and kitchens have the most potential for being the dirtiest, simply because they have great sources for microbe growth. But I don't see why it should be an issue if you clean up.

Consider this. What places in your house get cleaned the most?

I think glove boxes are silly. Unless the inoculation zone is filthy, they are superfluous.


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OfflineRogerRabbitM
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Re: Inoculation Jars. In Shower??? [Re: yessir]
    #14071296 - 03/05/11 02:51 PM (12 years, 10 months ago)

Quote:

yessir said:
I think glove boxes are silly. Unless the inoculation zone is filthy, they are superfluous.




We all work based on our experience level.  Lots of noobs think gloveboxes and flowhoods are silly, but three months later they're all either asking how to get trich spores out of their house or they go to a different hobby.
RR


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Offlineyessir
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Re: Inoculation Jars. In Shower??? [Re: RogerRabbit]
    #14071349 - 03/05/11 03:06 PM (12 years, 10 months ago)

Quote:

RogerRabbit said:
We all work based on our experience level.  Lots of noobs think gloveboxes and flowhoods are silly, but three months later they're all either asking how to get trich spores out of their house or they go to a different hobby.
RR




Well flow hoods are different. I would never bash the use of one of those. Glove boxes depend on the cleanliness of the box, correct? I see relatively little advantage to using a glove box over cleaning the work area.


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InvisibleDynGBreeD
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Re: Inoculation Jars. In Shower??? [Re: yessir]
    #14071368 - 03/05/11 03:10 PM (12 years, 10 months ago)

:popcorn:


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OfflinePsilocyentist
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Re: Inoculation Jars. In Shower??? [Re: yessir]
    #14071430 - 03/05/11 03:28 PM (12 years, 10 months ago)

Quote:

yessir said:
Quote:

RogerRabbit said:
We all work based on our experience level.  Lots of noobs think gloveboxes and flowhoods are silly, but three months later they're all either asking how to get trich spores out of their house or they go to a different hobby.
RR




Well flow hoods are different. I would never bash the use of one of those. Glove boxes depend on the cleanliness of the box, correct? I see relatively little advantage to using a glove box over cleaning the work area.




The advantage being still air? You can clean the hell out of your work surfaces but the air will never be as still as inside a glovebox, which will then help with contams falling onto your jars. How do you not see the advantage?:awewtf:


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Invisiblelipa

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Re: Inoculation Jars. In Shower??? [Re: yessir]
    #14071443 - 03/05/11 03:31 PM (12 years, 10 months ago)

Quote:

yessir said:
Quote:

RogerRabbit said:
We all work based on our experience level.  Lots of noobs think gloveboxes and flowhoods are silly, but three months later they're all either asking how to get trich spores out of their house or they go to a different hobby.
RR




Well flow hoods are different. I would never bash the use of one of those. Glove boxes depend on the cleanliness of the box, correct? I see relatively little advantage to using a glove box over cleaning the work area.





No.... still air boxes and glove boxes rely on still air to prevent airborne contaminates. We "always" clean our work surface no matter what we use. The moment you open a petri dish in the open air no matter how clean your surface area is you cause a vacuum and that vacuum pulls in airborne contaminates. There are gobs of contaminates floating around in the air in any room.  I laugh at these lab guys that say "Oh, I am good enough I do all my work in the open air". I would never take any of these guys serious. We culturist base our success on proper procedures. Not by chance.

Lipa


Edited by lipa (03/05/11 04:44 PM)


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InvisibleFungal growth
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Re: Inoculation Jars. In Shower??? [Re: lipa]
    #14071534 - 03/05/11 03:57 PM (12 years, 10 months ago)

yeah, i would say its near impossible to have zero air currents in an open room.
the currents coming frm your clothes and hands moving is like a gale to contaminates.
i desparately despised working in my glovebox, but with no flowhood it was a necessity.


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Offlinek00laid
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Re: Inoculation Jars. In Shower??? [Re: Fungal growth]
    #14071554 - 03/05/11 04:00 PM (12 years, 10 months ago)

make silicone injector ports.

then open air inoculation works a-ok.

a tube of clear silicone (you can use rtv if you want) costs like 5$ i think.


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