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Invisiblecyanara
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my petri is fruiting
    #14070164 - 03/05/11 10:06 AM (13 years, 18 hours ago)

Ok so I'm a little confused and aprehensive as to my culture fruiting in it's plate. Now I know its a sign of vitality, but it's also a sign that it wan't to end its life cycle.. what should or can I do?
this is only the first transfer from the ms master plate, I don't want to loose my genetics.:shrug:

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InvisibleDoc_T
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Re: my petri is fruiting [Re: cyanara]
    #14070177 - 03/05/11 10:10 AM (13 years, 18 hours ago)

You get to jump right to tissue cloning- move the sterile pin to a fresh plate.


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Invisiblecyanara
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Re: my petri is fruiting [Re: Doc_T]
    #14070219 - 03/05/11 10:20 AM (13 years, 17 hours ago)

ya I already did that, I took the pin from the master plate and after carfuly cuting in half I moved it to two seperate plates. you can check out the pics in the mycelium on agar thread.:super:
this is my second generation plates or the P-1's.
Plus correct me if i'm wrong, but can't you expand mycelium alot further if it hasn't fruited yet vs. mycelium that came from a clone seing how it has entered its sexual phase and is conciderably aged?

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OfflineRogerRabbitM
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Re: my petri is fruiting [Re: Doc_T]
    #14070224 - 03/05/11 10:21 AM (13 years, 17 hours ago)

Quote:

Doc_T said:
You get to jump right to tissue cloning- move the sterile pin to a fresh plate.




---> 7 days later
RR


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OfflineRogerRabbitM
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Re: my petri is fruiting (moved) [Re: cyanara]
    #14070228 - 03/05/11 10:22 AM (13 years, 17 hours ago)

This thread was moved from Advanced Mycology.

Reason:
Ooops, just realized this is in advanced. 

Moved to a more appropriate forum.
RR

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Invisiblecyanara
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Re: my petri is fruiting (moved) [Re: RogerRabbit]
    #14070232 - 03/05/11 10:25 AM (13 years, 17 hours ago)

is there anyway to get the mycelium to slow down?

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OfflineRogerRabbitM
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Re: my petri is fruiting (moved) [Re: cyanara]
    #14070256 - 03/05/11 10:31 AM (13 years, 17 hours ago)

Quote:

cyanara said:
is there anyway to get the mycelium to slow down?




Refrigeration.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblecyanara
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Re: my petri is fruiting (moved) [Re: RogerRabbit]
    #14070432 - 03/05/11 11:11 AM (13 years, 17 hours ago)

should i store the plate upside down to prevent contamination.

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InvisibleTranscendingLife
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Re: my petri is fruiting (moved) [Re: cyanara]
    #14070472 - 03/05/11 11:23 AM (13 years, 16 hours ago)

Quote:

cyanara said:
should i store the plate upside down to prevent contamination.




Nope, They should be in a sterile bag or sealed w/ parafilm...


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Re: my petri is fruiting (moved) [Re: RogerRabbit]
    #14070783 - 03/05/11 12:43 PM (13 years, 15 hours ago)

Quote:

RogerRabbit said:
Quote:

cyanara said:
is there anyway to get the mycelium to slow down?




Refrigeration.
RR




Whats the lowest temperature you should expose live mycelium to? Will it die if its below a certain temp?


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OfflineRogerRabbitM
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Re: my petri is fruiting (moved) [Re: esquaredx]
    #14070970 - 03/05/11 01:29 PM (13 years, 14 hours ago)

I have several shiitake and oyster cultures that have been in the deep freezer for several years.  They're still viable.  Keep cubensis in the 34F to 36F range.

Petri dishes should never be stored upside down.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineProfessorPinHead
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Re: my petri is fruiting (moved) [Re: esquaredx]
    #14070973 - 03/05/11 01:29 PM (13 years, 14 hours ago)

Just don't let it freeze and it will be ok. Sometimes even if it freezes you can bring it back from the dead.

But be careful! If it bites you, you may turn into a zombie :zombie:

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Offlineesquaredx
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Re: my petri is fruiting (moved) [Re: RogerRabbit]
    #14071178 - 03/05/11 02:24 PM (13 years, 13 hours ago)

Quote:

RogerRabbit said:
I have several shiitake and oyster cultures that have been in the deep freezer for several years.  They're still viable.  Keep cubensis in the 34F to 36F range.

Petri dishes should never be stored upside down.
RR




Sounds good. :thumbup:


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