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Offlinesomedaysoon
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questions for first grow
    #14066500 - 03/04/11 02:03 PM (13 years, 22 hours ago)

I've never grown anything before, and I have a tendency to overthink, so knowing very little to begin with and having so much available info can be a bad thing.  After spending many hours reading many, many threads, I went the lazy way and ordered a reasonably priced all-in-one kit with 12 pre-done cake jars, then ordered 3 syringes with B+ spores from a shroomery sponsor.  With all the threads I read, it seemed like building a full-blown glovebox may be overkill just to inoc a few jars, so I used the "over the edge of a surface" tek I saw a few different times (see avatar), the kitchen bar is a good height for me.  My orders arrived in the mail this week, so it was time to start this little experiment, this how I spent my afternoon......

1.  Bought 58qt sterilite container and bottle of 70% alcohol
2.  Put sprayer top on alcohol bottle, sprayed down inside of box, wiped it down, then let it air out while I put on long-sleeved shirt and latex gloves
3.  Sprayed and wiped down the counter top, then used alcohol-soaked paper towel to wipe down top, bottom, and side surfaces of 8 jars
4.  Flipped box onto counter, placed jars and syringe in box, taped the edges of the box to minimize airflow
5.  Assembled spore syringe (B+ strain), flamed needle until red hot, inoced jar 1 & 2
6.  Wiped needle with alcohol-soaked paper towel, reflamed needle, inoced jar 3 & 4.  Had trouble inocing #4, the needle tip was jammed
7.  By now noticed I had used almost 7cc's of spore solution, so I repeated step 6 for jars 5 & 6. Had trouble getting needle red hot again, looked like carbon build up, so I did what I could, finishing the syringe


At this point I noticed that I melted a small hole in the top of the box when flaming the needle, so between being generally nervous about everything and emptying the syringe, it seemed like a good time to stop.  I placed the inocced jars in a different, pre-heated tub for incubation, tore down the set up and started typing.  So now I've got a few questions that I'm hoping the experienced cultivators/mods wouldn't mind answering.

1.  How was my set up?  Any suggestions for improving?
2.  The two jars I wiped but didn't use, I re-wrapped the lids with foil and put them with the unopened jars in a dresser drawer.  How long should they be good for?
3.  With the build-up on the needle, is that a big risk for contamination?
4.  The unused syringes will be in the same dresser drawer as the unused jars, how long are they good for?

That's all the ?s for now, really depends on what happens over the next week or so to see how I did

thanks in advance for the inputs


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Offlinear1es
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Re: questions for first grow [Re: somedaysoon]
    #14067533 - 03/04/11 05:57 PM (13 years, 18 hours ago)

should flame needle in between each jar


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":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita

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InvisibleWise Toad
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Re: questions for first grow [Re: ar1es]
    #14067951 - 03/04/11 07:35 PM (13 years, 16 hours ago)

You don't even need alcohol to prepare your glovebox, its just a fire hazard. All glove boxes need is water to be sprayed inside and wiped around. You want the bottom to still be decently wet when your working in there as this will catch any contaminants that land there.

You don't need to tape the lid to the box, the airflow should be significantly impeded. It would be very tedious to do that every inoculation.

What temperature do you plan to incubate the jars at and what is your container?

If your having problems flaming needles you might want to either make an alcohol lamp or buy a butane powered microtorch which you could use quickly and efficiently inside of your GB.

2. The jars should last a week, maybe longer.

3. I get carbon deposits no matter what I flame with. Neither rusty lids nor carbon deposits on needles will cause any problems for mycelium.

4. I have spore syringes I got last summer that are still good today.

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Offlinemabachko
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Re: questions for first grow [Re: Wise Toad]
    #14068208 - 03/04/11 08:41 PM (13 years, 15 hours ago)

I'm pretty new at this, but if your syringe plugged then maybe ur needle is to small, try 16 gauge

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Offlinemabachko
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Re: questions for first grow [Re: mabachko]
    #14068333 - 03/04/11 09:16 PM (13 years, 14 hours ago)

Still working on my 1st grow. Bought 2 68 Qrt clear totes. the tops were white so I cut a hole in the top of them the size that a clear food wrap would cover, approx. 12 inches & duct tape the wrap to the openning.
For the glove box I cut 2 4inch holes for my arms to go in. That was it for the holes in the glove box.
I didn't have a PC at the time so I bought on line 2 spawn bags of rye. these were pre filled & sterilized.
Prior to inoculating I placed bags and syringe, bic lighter, a bottle of alcohol & paper towel in the glove box and sprayed the inside of the box with Lysol tile & bath spray. Then waited about 30 minutes.
After 30 or so minutes I flamed needle & wiped down with alcohol & inoculated bag 1. This is the scary part,, Seeing how the alcohol fumes had nowhere to escape to I was nervous about flaming the needle prior to inoculating bag 2, but I didn't blow up..
It has been 11 days and they are colonizing just fine without any contam's that I can see. I did wear long sleves & a pair of $1.25 dishwashing gloves washed down with alcohol prior to sticking my arms in the box.
My FC has about 20, 1/4 inch holes drilled about 3 inches up from the bottom & a 5/16 at the top where my air line will come in. U see I'm not a fan of venting so I am going to put an air line in that has a bubbler at the end of it into a glass of water. this will keep humidity up as well push out the bad air...Hope to have some good pics sonn. Good Luck

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InvisibleDoc_T
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Re: questions for first grow [Re: mabachko]
    #14068348 - 03/04/11 09:18 PM (13 years, 14 hours ago)

No flame in the glovebox. For reals.


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Offlinemabachko
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Re: questions for first grow [Re: Doc_T]
    #14068400 - 03/04/11 09:28 PM (13 years, 14 hours ago)

Thanks Doc T. as soon as I went to flame the needle the 2nd time I knew I messed up so I stood there for a few extra minutes held my face back & sparked the lighter. I thought that I had wrote it so that they wouldn't do a dumb thing like I did, but now we have both clearly stated that a flame source & alcohol liquid or vapor, SHOULD NOT BE NEAR EACH OTHER OR USED INSIDE A GLOVE BOX. Thanks Again, Newb's such as myself learn somthing new everyday hopefully.

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InvisibleDoc_T
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Re: questions for first grow [Re: mabachko]
    #14068491 - 03/04/11 09:46 PM (13 years, 14 hours ago)

Also, you don't need alcohol. Wash out the glovebox with warm soapy water, rinse, air dry.


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Offlinemabachko
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Re: questions for first grow [Re: Doc_T]
    #14068885 - 03/04/11 11:04 PM (13 years, 13 hours ago)

Hey Doc T I used Lysol Bath & tile cleaner in my glove box. it says 99.9% & it seemed to do the job as I have not seen any contams. that is a lot easier than soapy water. What do u think okay or is this asking for problems with my box down the road.

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Offlineafrosheen
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Re: questions for first grow [Re: mabachko]
    #14068905 - 03/04/11 11:08 PM (13 years, 12 hours ago)

I think Lysol is great as a household cleaner but don't see much value in mycology. Soap and water, alcohol swabs, and diluted vinegar water in a spray bottle are all very good at killing contams or keeping them choked down. Bonus is that all 3 are environmentally friendly and are cheap.


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Offlinesomedaysoon
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Re: questions for first grow [Re: afrosheen]
    #14082820 - 03/07/11 06:26 PM (12 years, 11 months ago)

Thanks for all the inputs, makes me a little less nervous, at least for now

I understand the comments about no alcohol and flame, the container did air out for about 5 minutes before I flipped it to inoc the jars, next time I'll probably go with soapy water just to be sure

Will remember to flame the needle for every jar next time, as long as it's ok to flame the needle just outside the container then quickly bring it inside, this and no alcohol should stop melting the top

The taping wasn't bad, three strips took < two minutes, the edge of the container was not flush with the counter top so it made me a little more at ease knowing that air could not flow up over the top of the jars

I've been checking the incubation container morning and night, temperature has been holding at a steady 80-82 degrees, 70% humidity

looking forward to the next couple of days to see what sprouts, will post pictures as soon as there's something to show, good or bad

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InvisibleFrankHorrigan
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Re: questions for first grow [Re: somedaysoon]
    #14083506 - 03/07/11 08:29 PM (12 years, 11 months ago)

Quote:

somedaysoon said:
1.  How was my set up?  Any suggestions for improving?





Get rid of your incubator.  70-75 degrees colonizes faster and is less likely to contam.

How much spore solution did you use per jar??  .5-1cc is good for each 1/2 pint.  It slows colonization when it gets overly wet.

Quote:

2.  The two jars I wiped but didn't use, I re-wrapped the lids with foil and put them with the unopened jars in a dresser drawer.  How long should they be good for?




I would use them asap or toss them.  Keep the glass tho.  It's cheaper and easier to just make your own 2-1-1 verm/BRF/water mix anyways.

Quote:

3.  With the build-up on the needle, is that a big risk for contamination?




No.  Squirt a tiny bit of solution through the tip after each flame to cool it and remove burned debris.  It was most likely a little BRF that stuck to the needle because it was hot when you inoculated.

Quote:

4.  The unused syringes will be in the same dresser drawer as the unused jars, how long are they good for?





A while.  I dont know the exact time, but i bet it would be longer in the fridge.

Don't worry so much and follow the teks.  They work great that way.

:mushdance:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (03/07/11 10:06 PM)

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Offlinesomedaysoon
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Re: questions for first grow [Re: FrankHorrigan]
    #14125717 - 03/15/11 04:15 PM (12 years, 11 months ago)

UPDATE
____________________
Checked jars on day 5, not much going on, temp still around 82

Checked again on day 8, 1/2" white spots all 4 injection sites on all 6  jars, not too sure, sort of looked like cobweb mold I've seen people talk about

Day 10 did not check, saw other teks about temps being higher, going to raise temp to about 85

Checked today (day 12), and there's good growth!!  4 of the 6 are at least 50% covered, the other two are not far behind, rearranged jars to give slower jars a chance to catch up.

Looking back, jars have followed the pattern I saw under the one the the 5 stars teks that I saw and cannot find now

I keep seeing posts that make me think the jars are not going to last so I'm going to set up hood and inoc the other 6 jars, hope I haven't waited too long.......

Inoccing jars was easier this time, still used 1 whole syringe for only 6 jars, better too much than too little, numbered jars to keep track of batch 1 and batch 2

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InvisibleFrankHorrigan
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Re: questions for first grow [Re: somedaysoon]
    #14126312 - 03/15/11 06:01 PM (12 years, 11 months ago)

seriously man, ditch the incubator and give them a little indirect light and room temps each day.

they will colonize super super fast and be far less likely to contam.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlineafrosheen
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Re: questions for first grow [Re: FrankHorrigan]
    #14127411 - 03/15/11 09:21 PM (12 years, 11 months ago)

Quote:

Inoccing jars was easier this time, still used 1 whole syringe for only 6 jars, better too much than too little,




Wrong, with spores, especially in BRF jars, you want maybe 1/2CC per injection hole. This is a few drops barely dribbling out. The more you add, the slower it will be to grow initially, and you run the risk of throwing your water content off. A drier sub is preferable to a wet sub, and less is more when it comes to spores.


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Offlinesomedaysoon
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Re: questions for first grow [Re: afrosheen]
    #14197617 - 03/28/11 12:26 PM (12 years, 11 months ago)

did not mean to use a whole for syringe for 6 jars, especially the second time, I was originally hoping to do all twelve jars with only one syringe.  Not good with a needle I guess, practice makes perfect........

day 24 update
Second set of six jars are coming along nicely so far, then I looked at first 6 jars, thought they were fully rooted so I set up fruiting chamber.  Problem is I left the foil loosely on top of the jars while they incubated so I didn't notice the top 3/4" of jar still did not have roots.  So obviously I had problems when I opened up first jar and tried to remove the cake to put in fruiting chamber.  I put the top back on, put jar back in place with remaining jars, removed the foil off all the jars.  Now I'm even more nervous I f'd things up, contamination is what I'm most worried about.  All jars are now out of incubator, at room temp (about 76), waiting for roots to completely take over cake.

BTW, I really appreciate everyone's comments, I've learned a lot, I was wondering how to get I get direct dialogue with a moderator.  I'm here not only to learn from everyone but also to learn from the best, so I'd like to know who I get them to review/comment on my post.

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Offlineafrosheen
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Re: questions for first grow [Re: somedaysoon]
    #14198840 - 03/28/11 04:56 PM (12 years, 11 months ago)

The mods stay out of discussions 99% of the time. The people to look for accurate help have high post counts and trusted cultivator tags. Some people with high post counts have just been bullshitting in the public forum for 5 years so that's not always an accurate gauge.


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Offlinesomedaysoon
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Re: questions for first grow [Re: afrosheen]
    #14268311 - 04/10/11 06:14 PM (12 years, 10 months ago)

starting to get unhappy.....its been over a week since I put the first six jars in the fruiting chamber, temp/humidity has been steady around 80 degrees and 90% humidity, getting plenty of light as chamber is in front of a window (tinted so nosy people cannot see in).  Still don't seem to have any growth, now it looks like I'm getting some white mold growing on top of mycelium.  How can I tell if its positive growth or contamination?!?

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Offlineafrosheen
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Re: questions for first grow [Re: somedaysoon]
    #14268318 - 04/10/11 06:16 PM (12 years, 10 months ago)

You'll know if it's bad in 2-3 days. In the meantime, have patience. Mist and fan a few times a day.


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Offlinesomedaysoon
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Re: questions for first grow [Re: afrosheen]
    #14277354 - 04/12/11 10:49 AM (12 years, 10 months ago)

definitely mold, one is starting to turn from white to green.  Now what?  I know I have to clean/dry vermiculite, wipe down terrarium, then re-moisten vermiculite, this time with some hydrogen peroxide.  I'm sort of in a hurry, I had one last jar of the first six , it now has a mushroom growing and it's not out of the jar, so there's still hope for the second batch.....And any tips on preventing the same mold the second time

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