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Invisiblejokefox
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AB cardboard, Half tek (poor rich mans agar dish)
    #14068294 - 03/04/11 09:05 PM (12 years, 10 months ago)

The best way ive learnt to save AB ,
since we all know how much our agar offspring is worth,

Materials
  • Agar broth (AB)
  • Cardboard squares, (cut squares from a cardboard box, remove any tape glue or other crap)
  • 1/2 pint jars (i like these best)



Step 1. - place a few squares in jar,
step 2. - pour only enough AB/LC in jar to get the squares wet, (any extra liquid can be dumped out, or kept in
to make a liquid culture starter,

so pretty much like this



then PC for and hour let cool and drop spores,
best thing about this is the amount of agar you save


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InvisibleDoc_T
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox]
    #14068314 - 03/04/11 09:12 PM (12 years, 10 months ago)

Agar is $1.69 a pack.

Plus, why are you going from agar to agar? Why not agar->grains->g2g if you want to conserve materials?


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Invisiblejokefox
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: Doc_T]
    #14068350 - 03/04/11 09:19 PM (12 years, 10 months ago)

i use it to save on agar broth , it helps me ,


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InvisibleDoc_T
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox]
    #14068362 - 03/04/11 09:21 PM (12 years, 10 months ago)

Agar broth? Is that like agar? Or like LC? Why are you using more than a tiny bit of either?


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Invisiblejokefox
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: Doc_T]
    #14068380 - 03/04/11 09:24 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Agar broth? Is that like agar? Or like LC? Why are you using more than a tiny bit of either?




where have you been

my agar broth is A lc type deal with agar in it

i add this to cardboard and it makes transferring alot easier for me when im in a glovebox


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Invisible13shroomsM
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox]
    #14068389 - 03/04/11 09:26 PM (12 years, 10 months ago)

:stoner:


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Invisiblebiologys
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: 13shrooms]
    #14068581 - 03/04/11 10:05 PM (12 years, 10 months ago)

i never really saw the point for an 'agar broth' myself, but hey to each his own :peace:


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Invisiblejokefox
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: biologys]
    #14068823 - 03/04/11 10:50 PM (12 years, 10 months ago)

when agar broth is used as a liquid culture base, when shaken , the air bubbles stay suspended
and i noticed the mycelium loves this, also when inoculated creates a nice surface for the mycelium
to speed across, kinda like a boost,

when done on cardboard like this with some agar broth to moisten the cardboard,
you get the mycelium witch grows over the cardboard fast and can be used to innoc, bags, or jars or whatever

i use a glove box, and its a pain to have to cut a wedge and then put it in the bag, its alot easier
to grab a square of cardboard with tweezers and put that into a bag,

i also buy disposable scalpels since i work in a glove box and cant use a flame,
i dont like to take caches and a little extra money on supplies goes a long way
even more so when you use other ideas to make it last,

be as close minded as you want, but i was right about the agar broth ,

that is all


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Invisiblebiologys
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox]
    #14068853 - 03/04/11 10:58 PM (12 years, 10 months ago)

im not being closed minded at all, I could of easily said "i dont see any point in this, its fucking stupid" or something along those lines...I simply made a statement and said everyone has their own opinions, which is try...it works for you, and you like it so good for you, i myself find agar, or normal LC's to be just as simple so thats my choice, just like the 'agar broth' is yours..


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Invisiblejokefox
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: biologys]
    #14068861 - 03/04/11 11:00 PM (12 years, 10 months ago)

i never ment your being closed minded, i just mean a general statement,
and i never invented agar broth ,

its practiced in labs,

some things catch on i guess, and some people are scared to move onto new paths,

but cardboard is already known to be good for spores to germinate on since it holds off some contams


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Offlineafrosheen
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox] * 1
    #14068879 - 03/04/11 11:03 PM (12 years, 10 months ago)

Jokefox, next time you do that, throw some dry coir in there with it instead of cardboard. I bet that'd work also since the myc can dig in and coir is hard to contaminate.


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InvisibleModularMind
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: jokefox]
    #14068962 - 03/04/11 11:21 PM (12 years, 10 months ago)

Do you expect the mycelium to eat that hair?
Haha. Gross.


Edited by ModularMind (03/04/11 11:22 PM)


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Invisibleslapphappypill
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: afrosheen]
    #14068964 - 03/04/11 11:21 PM (12 years, 10 months ago)

Wouldn't the coir be a better idea? that way the myc already is introduced to coir before it even jumps into the grain? That soudns like it'd give many more innoc points than cardboard.

Afro you're a smart guy :hamsterdance:


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FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
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Offlinefaceyneck
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: afrosheen]
    #14080080 - 03/07/11 08:56 AM (12 years, 10 months ago)

Quote:

afrosheen said:
Jokefox, next time you do that, throw some dry coir in there with it instead of cardboard. I bet that'd work also since the myc can dig in and coir is hard to contaminate.





I would guess that's a pretty good way to get bacterial contamination.

The reason why wet grains are bad is because that swampy solid-liquid-bubbly mess that's created by pooling water, and nooks and crannies created by the grains.

If one were to add coir to agar broth - seems that would cause very much the same environment. :shrug: Probably even more conducive to bacterial growth, actually.


Hey, Joke-fox - I may have missed something, but - what do you do with the pieces of cardboard? As in - where do you place them for colonization?

EDIT_ do you just PC several pieces of cardboard with the broth stuff in there? That seems risky to me - nowhere near as bad as coir, but - still, a rather large impedance for airflow/ probable trapped air. :justdontknow:

If interested, I highly recommend this thread. It really opened my eyes to how and why certain environments breed bacteria. :kenthumbup:


Edited by faceyneck (03/07/11 09:03 AM)


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Offlineafrosheen
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: faceyneck]
    #14082509 - 03/07/11 05:40 PM (12 years, 10 months ago)

Enormous difference between wet unsterilized grain and coir. Coir comes out of the package washed a few times and probably practically sterile already. Put it through a PC cycle in the agar broth and I'd be amazed to find any critters surviving on it. They don't even like to grow on it when it's wet and sitting around in the open air.


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Offlinefaceyneck
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: afrosheen]
    #14082540 - 03/07/11 05:45 PM (12 years, 10 months ago)

Quote:

afrosheen said:
Enormous difference between wet unsterilized grain and coir. Coir comes out of the package washed a few times and probably practically sterile already. Put it through a PC cycle in the agar broth and I'd be amazed to find any critters surviving on it. They don't even like to grow on it when it's wet and sitting around in the open air.




I wasn't talking about unsterilized grain. I was talking about wet sterilized grain.

Whether or not the coir is cleaner than grains initially shouldn't make a difference on account of the sterilization process.

Also, the issue is in the impedance of airflow, not cleanliness of the substrate, as the risk of contamination would come from the inoculation process. Even inoculating in front of a flowhood is not a truly sterile process.


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OfflineRogerRabbitM
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: afrosheen]
    #14082599 - 03/07/11 05:55 PM (12 years, 10 months ago)

Quote:

afrosheen said:
Enormous difference between wet unsterilized grain and coir. Coir comes out of the package washed a few times and probably practically sterile already. Put it through a PC cycle in the agar broth and I'd be amazed to find any critters surviving on it. They don't even like to grow on it when it's wet and sitting around in the open air.




This is why it shouldn't be used, actually.

On agar, we want contaminant spores to germinate and grow so we can transfer healthy mycelium away from it.  If you have a medium that contaminant spores don't germinate, they're preserved intact until you transfer to grains, and then they ruin your day. 

Remember, agar isn't for expanding mycelium.  It's for cleaning up from contaminants to get a pure culture.
RR


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Offlinefaceyneck
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: RogerRabbit]
    #14082645 - 03/07/11 06:02 PM (12 years, 10 months ago)

That's a good point, about the coir.

I this instance, I believe the purpose of the cardboard agar snot is as an inoculant, not cleaning up a culture however. :justdontknow:


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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: faceyneck]
    #14082798 - 03/07/11 06:22 PM (12 years, 10 months ago)

Good stuff, learned something new today. :thumbup:


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Offlinefaceyneck
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Re: AB cardboard, Half tek (poor rich mans agar dish) [Re: afrosheen]
    #14082912 - 03/07/11 06:40 PM (12 years, 10 months ago)

Indeed. :kenthumbup:


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My Meyers-Briggs Personality: INTJ
New growers, or anyone else just needing help; I'm always glad to help right here. :grin:


We give cultivation advice here. :super:


AMU Q & A - We're glad to help
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