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bdzsmkr365247
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Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
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Re: Q about temp for incubation [Re: Primal Call]
#14065964 - 03/04/11 12:00 PM (13 years, 1 day ago) |
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egh i am patient,, mostly.. i have done this before so i am very aware that this is a hobby of EXTREME patients!!
i am going to give them more time as previously stated!
temp is Currently 66F
i am impatient when i have to wait for my spores to get delivered though HAHA
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Quote:
i am very aware that this is a hobby of EXTREME patients!!
That's what doctors in acute care hospitals do, but it's not a hobby.  RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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bdzsmkr365247
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Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
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Re: Q about temp for incubation [Re: RogerRabbit]
#14070246 - 03/05/11 10:28 AM (13 years, 6 hours ago) |
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HAHAhaha SENIOR RABBIT!! thanks for posting on my thread!! i consider you al knowing...
current temp: 68
jar (i) has two fuzzyballs now jar (a) still only has one and the rest are still up in the air..
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bdzsmkr365247
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Registered: 05/18/10
Posts: 301
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well,, the temp is at a steady 70-72F i am so stoked, two jars are starting to show col. but the rest are still nothing, i just thought i would keep y'all updated
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bdzsmkr365247
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Registered: 05/18/10
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I did a multi spore injection o the jars, the needle got clogged a few times, every time i injected i squirted some H2o out, is it possible that some jars may not have gotten spores??
the reason i ask this is cause two 1/2 pints are showing signs of colonization and the rest are not doing a thing
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jmello



Registered: 01/27/08
Posts: 264
Last seen: 5 days, 2 hours
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Quote:
bdzsmkr365247 said: I did a multi spore injection o the jars, the needle got clogged a few times, every time i injected i squirted some H2o out, is it possible that some jars may not have gotten spores??
the reason i ask this is cause two 1/2 pints are showing signs of colonization and the rest are not doing a thing 
Its not likely cuz if you got a syringe from a vendor there are thousands of spores in a syringe...even if you forget to shake before innoc (ive done it plenty) your chances are still high for success. I have gotten an old syringe that took an exceptionally long time to show any signs of growth, longer than times your dealing with. I would do g2g with the jars u have colonizing now, seems like the myc is a little less finicky with what it wants to eat
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jakedorset
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Registered: 02/19/11
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Last seen: 12 years, 10 months
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Re: Q about temp for incubation [Re: jmello]
#14081609 - 03/07/11 03:04 PM (12 years, 11 months ago) |
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i'm sure you got some spores in there. remember, you only need 2, and there's billions in that syringe.
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bdzsmkr365247
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Registered: 05/18/10
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Re: Q about temp for incubation [Re: jakedorset]
#14083326 - 03/07/11 07:49 PM (12 years, 11 months ago) |
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the jars i knocked up are BRF not grain, i do not have pressure cooker to sterilize grains, could i do a BRF to grain any suggestions? i am planning on doing a bulk dubtub and some monos.
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bdzsmkr365247
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Registered: 05/18/10
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Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
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well,,,, three of the 1/2 pints are colonizing i am starting to think the rest are goners .. i knocked up 12 pint size jars . 6 orisa and 6 TC!
the temps are ranging from67-70.. unfortunatelyyy.....
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Primal Call
Earth Mage



Registered: 09/05/10
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those temps are fine... sure, on the low side, but this is a good thing when considering contams thriving in 80F+ situations.
again, you say you are patient, but it's only been just over 2 weeks since your inoculation. sure, this is not ideal, but it can happen... I recently had a jar of CR take just under 4 weeks simply to germinate. even after it did, it colonized SUPER slowly on the grain at temps around 72F, so I did a G2G and now it's charging through 5qt jars. maybe it was the moisture content, the temps, the phase of the moon, or the position of the 6th ring on saturn... but I'm sure glad I didn't give up on it.
do what you will, but giving up hope on them when they aren't yet contamed (at least not obviously) seems wasteful IMO 
on BRF to grain, it probably isn't a good idea without a PC. you'd be better off spawning BRF to pasteurized bulk
good luck with it. sending vibes your way
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