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Invisiblecyanara
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lc questions
    #14065902 - 03/04/11 11:45 AM (12 years, 10 months ago)

ok so there is alot of info and teks out there... I have some nice clean plates, would it be better to throw the agar in some lc with like a chunk of glass to help break it up, or do the whole blender thing...


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InvisibleDoc_T
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Re: lc questions [Re: cyanara]
    #14065980 - 03/04/11 12:04 PM (12 years, 10 months ago)

Agar-> grain-> grain LC-> LC. :thumbup:


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InvisibleSlimz
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Re: lc questions [Re: Doc_T]
    #14066086 - 03/04/11 12:30 PM (12 years, 10 months ago)

i agree.. never LC from agar, you have no clue what you have .. go to a grain grow and then once you know what you have make a clean LC from the grain grow.


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Invisiblecyanara
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Re: lc questions [Re: Slimz]
    #14066203 - 03/04/11 01:00 PM (12 years, 10 months ago)

ok. even though I've subcultured into multiple dishes with no contams?


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InvisibleDoc_T
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Re: lc questions [Re: cyanara]
    #14066240 - 03/04/11 01:10 PM (12 years, 10 months ago)

Agar->Lc is fine too.


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Invisiblecyanara
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Re: lc questions [Re: Doc_T]
    #14066363 - 03/04/11 01:38 PM (12 years, 10 months ago)

doc what would be a good recomendation as far as agar>lc?
how would you go about it?


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InvisibleDoc_T
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Re: lc questions [Re: cyanara]
    #14066482 - 03/04/11 02:01 PM (12 years, 10 months ago)

Open LC, drop in agar wedge.


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Invisiblecyanara
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Re: lc questions [Re: Doc_T]
    #14066539 - 03/04/11 02:12 PM (12 years, 10 months ago)

cool.. do I need to put anything in there to break up the mycelium from the wedge?
Do you use an injection port?


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Re: lc questions [Re: cyanara]
    #14066554 - 03/04/11 02:16 PM (12 years, 10 months ago)

I use stirbars in LC. What would you inject?


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Invisiblecyanara
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Re: lc questions [Re: Doc_T]
    #14066572 - 03/04/11 02:19 PM (12 years, 10 months ago)

not to inject but to pull the lc out. also I don't have a stir bar so i'll have to use my hands to aggitate.:mushroomgrow:


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Re: lc questions [Re: cyanara]
    #14066603 - 03/04/11 02:23 PM (12 years, 10 months ago)

I use needleless withdrawal ports: http://www.shroomery.org/forums/showflat.php/Number/11491601#11491601
But an RTV port will work. Put in a marble to stir with.
Polyfil is an adequate filter if you're careful. Don't fill your jar very full either. Less than half.


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Invisiblecyanara
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Re: lc questions [Re: Doc_T]
    #14066791 - 03/04/11 02:57 PM (12 years, 10 months ago)

I was planing on using a quart jar with 2 cups of water.
Also do you have to replace the lid every time with the tyvek filter and needle tip?


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Re: lc questions [Re: cyanara]
    #14066872 - 03/04/11 03:17 PM (12 years, 10 months ago)

I'm still using the same lids from the build pics. Took the tyvek off, I use polyfil these days.
But there's lots of ways to grow LC, I'm sure you can figure it out from here.


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