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Invisiblebootster
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Registered: 02/22/11
Posts: 1,531
Myc water transfer from MS?
    #14065077 - 03/04/11 08:24 AM (12 years, 10 months ago)

I have seven cooled rye jars that I'm considering knocking up with some colonized MS B+ from a purchased spore syringe.

Should I grow out the B+ and get a good print and start from there or just start a new MS strain with the seven jars?


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Invisiblehiggledy-piggledy

Registered: 08/24/10
Posts: 966
Re: Myc water transfer from MS? [Re: bootster]
    #14065336 - 03/04/11 09:32 AM (12 years, 10 months ago)

:albundy:

i know this is the question
Quote:

bootster said:
Should I grow out the B+ and get a good print and start from there or just start a new MS strain with the seven jars?




but... what is the question?

can you rephrase that?


Quote:

bootster said:
...I'm considering knocking up with some colonized MS B+ from a purchased spore syringe.



colonized what?


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Invisiblebootster
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Registered: 02/22/11
Posts: 1,531
Re: Myc water transfer from MS? [Re: higgledy-piggledy]
    #14065447 - 03/04/11 10:00 AM (12 years, 10 months ago)

Remember now,I am a noob.

My question is,I want to know if it is better to do a myc water transfer to grains with a master culture proven to be good,or is it OK to do a MS where it will be a crap shoot as to the "strain".

I have 6Qts of fully colonized B+ (from MS syringe) that I am planning on birthing to bulk  manure substrate this weekend. I was wondering if it would be worth it to draw some myc water from one of the jars and knock up 7 prepped rye jars. I could always start some different strain with the jars and isolate a mature fruit from the B+ and then use that in the future.

I hope that I cleared things up a bit.


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Offlinek00laid
NEMO
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Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
Re: Myc water transfer from MS? [Re: bootster]
    #14065450 - 03/04/11 10:02 AM (12 years, 10 months ago)

multispore from sponsors is fine.

it will all fruit.
you cannot determine potency from the rhizomorphic characteristics anyways.


--------------------
AMU - AMU Q & A - MyVideo Teks!


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Invisiblebootster
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Registered: 02/22/11
Posts: 1,531
Re: Myc water transfer from MS? [Re: k00laid]
    #14065524 - 03/04/11 10:24 AM (12 years, 10 months ago)

I saw threads of cloning "the big one" and making cultures for it as a master. I didn't know if it was worth it to go that route,or use what I have (sponsor syringe). I really don't want to start another strain at this point in time,but have the jars prepped.


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Invisiblehiggledy-piggledy

Registered: 08/24/10
Posts: 966
Re: Myc water transfer from MS? [Re: bootster]
    #14065745 - 03/04/11 11:11 AM (12 years, 10 months ago)

Quote:

bootster said:
...or use what I have (sponsor syringe).



this.

making a grain lc is too much work compared to just use your ready syringe.
at the end of the day its all multispore.

... and while your 7 jars and your bulk is colonizing you can take the time to read about liquid cultures and cloning "the big one".

:goodluck:


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Invisiblebootster
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Registered: 02/22/11
Posts: 1,531
Re: Myc water transfer from MS? [Re: higgledy-piggledy]
    #14065985 - 03/04/11 12:05 PM (12 years, 10 months ago)

Ive done a lot of reading about that. The sponsor syringes that I have are different from B+. I don't want to start another strain ATM.

I do have a little B+ left in the original SS. I want to do agar and have been reading a lot about it. I guess I'll just do the myc water thing with the B+. Thanks.


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