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OfflineLurkingWizards
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PE tubs col. w/o bag in open room (pics updated)-Brain Harvest!
    #14064369 - 03/04/11 01:44 AM (12 years, 10 months ago)

Updated with fruiting pics (middle of day ) HARVEST! :zombie:

just spreading the knowledge....

both tubs were created on 2-26 (near 1 am), PE MS->LC->WBS->coir/verm
both tubs were fruited on 3-8

tub A - colonizing (3-1)
4 qts PE 'young' spawned to 8 qts coir/verm mix [about 12 qts total]

same tub A, but 60 hours later, after sitting in an open air room, polyfil in holes with tape applied lightly over the polyfil(just a test)

same tub A, after another 51 hours

Tub A: 10 hours into fruiting conditions (3-8)

Tub A 3-15 (~179 hours into fruiting conditions, middle of day 8)

Tub A 3-16 (~207 hours into fruiting - pins/blobs)

Tub A (~227 hour in, finishing day 10)

and same tub A (~250+ hours into fruiting, middle of day 11)

Tub A (middle of day 12)

Tub A (middle of day 13)

Tub A (middle of day 15)-almost time to harvest a few

here is a close up if the upside down guy... reminds me of Salvador dali or something, but PE does that to me :smile2:

should I try to clone this mutant to see if it can be repeated? I just want to see what kind of fruits it would put out...
Tub A (middle of day 17 pics partial harvest)
101 wet grams
Tub A - rest of 1st flush... (middle of day 18)
142 wet grams


I also picked another 15 wet grams (not pictured) later right before the dunk phase...
101 + 142 + 15 = ~ 258 wet grams 1st flush (picked-off all of the small brain-bits, but didn't weigh the 'scraps')

hope the next flush is better...


UPDATE: Second flush Tub A - (about 10 days since 1st flush/dunk)

scales broke, so no wet weight was taken...
___________________________________________________________________________________________________
---------------------------------------------------------------------------------------------------
tub B - colonizing (3-1)
4 qts PE 'old' spawned to 5 qts coir/verm mix [about 9 qts total]

same tub B, but 60 hours later (2.5 days)- same open room conditions

same tub B, another 51 hours later

Tub B: 10 hours into fruiting (3-8)

Tub B 3-15 (~179 hours into fruiting conditions, middle of day 8)

Tub B 3-16 (~207 hours into fruiting - pins/blobs)

Tub B (~227 hrs in, finishing day 10)

and (~250+ hours into fruiting, middle of day 11)

Tub B (middle of day 12)

Tub B (middle of day 13)

Tub B (middle of day 15)-almost ready to pick some brains! :zombie:

another close-up of the madness...

Tub B (middle of day 17) before and after harvest
121 wet grams
Tub B - rest of 1st flush (middle of day 18)
152 wet grams

121 + 152 = ~ 273 wet grams 1st flush (I didn't weigh what I didn't keep, but lots of brain-bits were removed) - flush phase

hope the next flush is better, lol

UPDATE: Second flush Tub B - (about 10 days since 1st flush/dunk)


scales broke, so no wet weight was taken...

______________________________________________________________________________________________
I'm just adding to the lit., helping to confirm known practices...

some background....
I started two PE MS LC tubs... one tub was created with 'older' spawn, while the other was made with 'younger' spawn

the low down...
each tub was created using the same 'genetic-spawn', but the 'younger' spawn was created on 1-26 from a pint of the 'older' spawn (created on 1-9)... kind of confusing :picard:

so the 'older' spawn has been colonizing from g2g transfer since 1-9, they were 100% by the 22nd and left to consolidate(for about 5 weeks), then on the 26th, one single pint (of said 'older' spawn) was expanded to another 5 quarts(via g2g)... they were colonized by 2-10... waited two weeks for consolidation in jars... both tubs were created on 2-26

the 'young' spawn(~4 weeks in jar) tub (A) was mixed at 1:2(spawn:sub) with coir/verm
the 'older' spawn(~7 weeks in jar) tub (B) was mixed ~ 1:1

this is partially an experiment just to see what happens... I have my own theories as to what kind of outcome will result, but I would like to hear from the community and hopefully try to update this post as everything runs its course.

theories/riddles abound

thanks for checking this out! :murray:


Edited by LurkingWizards (04/05/11 02:33 PM)


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Offlineshroomzi8
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Re: tubs colonizing w/o bag in open room (pics) [Re: LurkingWizards]
    #14064493 - 03/04/11 02:46 AM (12 years, 10 months ago)

sorry to be a touch off topic. your crazy lemons pic is brilliant. mahalos.


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OfflineProfessorPinHead
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Re: tubs colonizing w/o bag in open room (pics) [Re: shroomzi8]
    #14064699 - 03/04/11 04:40 AM (12 years, 10 months ago)

Lookin' very good bro' :thumbup:


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) [Re: ProfessorPinHead]
    #14074275 - 03/06/11 04:45 AM (12 years, 10 months ago)

Pic updates!
looks almost ready to me :learyharvard:
day 8-9
tub A                                                          tub B


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OfflineProfessorPinHead
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Re: tubs colonizing w/o bag in open room (pics) [Re: LurkingWizards]
    #14074310 - 03/06/11 05:12 AM (12 years, 10 months ago)

Tomorrow pull one of those plugs on each side length wise diagonally to each other.

That tiny uncolonized  spot needs a day to consolidate.


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) [Re: ProfessorPinHead]
    #14077801 - 03/06/11 08:35 PM (12 years, 10 months ago)

EDITED: with pic and extra background conspiracy theories...

I figure I'd wait about 9-10 days total, the last pics were at about 200 hours into colonization... the end of tomorrow will be the end of day 10. :smile2:

I let the 'jar-spawn' that was used to create these tubs colonize and consolidate longer than usual (colonized in 2-3 weeks and consolidated for another 2-4 weeks).

I've noticed in the past that the number/percentage of 'blob-like' fruits seems to decrease when I have allowed the tubs to consolidate longer than usual when using MS spawn.  Sometimes even skipping their first flush :shrug: and not fruiting for 20+ days... then, bam!, 'normal-looking-PE' fruits skipping the usual 1st flush blobs from MS.

this pic is just an example of a small experimental (PE MS) 4 qt (1qt spawn:3qt coir/verm) tub showing the results from 3 weeks of colonization/consolidation in tub, and 1st flush fruits after another 4 weeks?, but normal-ish PE fruits, and zero mutated-blobs (I also neglected this tub more than usual... almost forgot about it, lol, was just a test/experiment tub anyways) :smile:

here is another example of a tub that was left to consolidate for an extended period of time before fruiting conditions were induced...

1st Flush about 3-4 weeks into fruiting
===================================================================================

So, for the experiment factor in this thread...
this time around I let the jars consolidate longer... and the 'oldest-jars'(7 weeks) were spawned to a more favorable ratio of the coir/verm mixture.  Needless to say, both tubs have colonized at about the same rate, which was to be expected with such high spawn-ratios.

I'll update the progress in the weeks to come... thanks again everyone.


Edited by LurkingWizards (03/15/11 02:53 PM)


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) [Re: LurkingWizards]
    #14085853 - 03/08/11 09:44 AM (12 years, 10 months ago)

Update pics

introduced to fruiting conditions... i.e. removed mass of polyfil from 2 diagonally-opposite holes (one on each side), still sitting in same position in the room.

Tub A (young spawn~4 weeks in jars) after 10 hours in fruiting cond. (240 hours of colonization/consolidation time)


Tub B (old spawn~7 weeks in jars) same treatment



the next series of experiments with PE MS will involve 'older-jar-spawn' and 'longer colonization/consolidation times' when expanded to bulk sub. combining my last two experimental efforts.

The point of this is to spread a better understanding of what affects the fruiting potential of MS PE.  I have nothing wrong with the blob-like fruits from PE-MS, and encourage others to work with isolates or clone 'proper' fruits (which usually come from the second flush onwards).

I can almost get 'proper' first flush fruits using MS... it just takes longer to colonize/consolidate, the fruits still grow at the same rate from pin to harvest (but takes 2+ weeks to even pin) it's just weird, but I guess the age of the myc. has something to do with it...

I currently have been working on many PE clones via stem->LC->WBS as future projects, so the MS experiments are coming to a close.  Looking forward to these clones, they colonize fast via G2G... I always have to wait about 3 weeks from LC though :shrug:

EDIT:  I should also add that the origin of this particular PE was from the very last 2 cc's of a MS syringe.  All previous attempts had failed over and over... those last two cc's generated 2 different LCs, which have been the basis of all PE MS experiments to date.  One of the LCs does seem to preform differently, neither are really better... but one had the affinity for taking forever to fruit (and producing far fewer blob-like fruits, hence the experimentation due to curiosity)
:murray:
Thanks again for checking this out


Edited by LurkingWizards (03/08/11 10:08 AM)


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) [Re: LurkingWizards]
    #14124559 - 03/15/11 10:56 AM (12 years, 10 months ago)

179 hours into fruiting conditions...

I need to get a better 'picture-taking-device'... :smile2:
this is true

because it looks like rise of the brain-blobs down there, but my camera blurs close-ups and wouldn't focus for :poop:

Tub A:


Tub B:


thanks again for following along


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OfflineGonkulator
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14124583 - 03/15/11 11:03 AM (12 years, 10 months ago)

Did you use aluminum foil as a liner in your tubs? Also, when you say "open room", does that mean something like the tub colonized in a normally ventilated / lighted room (such as a bedroom; as opposed to some of our ghetto closet-projects)? Or does it mean something else? When I dive back into my projects, I will probably utilize monotubs in accordance with some of the advice thrown out by Professor Pinhead and Facey a la ...that heated thread from a few weeks ago, lol.

edit: spelling


Edited by Gonkulator (03/15/11 11:05 AM)


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: Gonkulator]
    #14124707 - 03/15/11 11:33 AM (12 years, 10 months ago)

Yeah, the foil is easier for me to manipulate into liners sometimes... and was what I had on hand at the time. Nothing against bags, I just didn't have any of the thick, non-see-through kind, lol :smile2:

And yes, the tubs were just sitting in a room with a window/shaded sunlight and polyfil in the holes... that's what I meant by 'w/o bag', like the tub is not sealed in a bag hiding in the dark, :wink:

it's funny because I had started these tubs with this purpose of experimentation for my own sake...

Then I read about that same thread and how a few members have already been debating the topic... it was kind of cosmic for me because I had been playing with this idea for a while, but I also have many other variables at work in this particular experiment (namely the older/younger spawn nonsense, that and spawn/sub. ratios)

I'm not really wanting to prove or deny anything, just trying to understand the whole non-blob fruits I have been getting from the first-flushes of a few of my PE MS tubs. If I let them colonize/consolidate longer in the tubs, I have to wait longer for the first flush, but I end up with zero brain-blobs :shrug:


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OfflineGonkulator
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14124789 - 03/15/11 12:59 PM (12 years, 10 months ago)

Delightful! I'm happy for your success. I wasn't trying to bring any of the controversy that seemed to sometimes flare up in the other thread at times into yours; I just wanted to know the specifics of your colonizing tub. So no taped holes / sealed lid or anything like that? I like the simplicity of it. I'd love to give an attempt at Penis Envy, if nothing else for the challenge of tackling a more, shall we say, dastardly strain.

I understand printing those dongs can be tricky, but if you're sending prints to your infatuated fanbase, consider me a candidate. I'd be more than happy to send something back in return once my operations resume from their current state of dormancy (the result of being inbetween residencies, I'm afraid).

edit: grammar


Edited by Gonkulator (03/15/11 01:00 PM)


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: Gonkulator]
    #14143669 - 03/18/11 02:59 PM (12 years, 10 months ago)

Quote:

Gonkulator said:

I understand printing those dongs can be tricky




I have yet to obtain my own print, lol... I might try swabbing gills -> agar eventually, but I have some clones from PE fruits in progress (made a bunch of separate LCs from inner-stem myc.) I would recommend obtaining a print/syringe from a vendor because of the difficulty in printing (they seem to have spores, just rarely drop any of them... damn mutants :oldman2:)

I'm pretty sure there is a method of creating 'non-sterile' spore-syringes that must be started on agar and transferred (found somewhere on this forum), but it works from what I read about the procedure. I just don't have time right now for more agar work, lol, but in the coming months... we'll see :shrug:

I also have updated fruiting pics :smile2:

tub A (3-17)                                                tub B (3-17)

Tub A (3-18)                                                Tub B (3-18)


LW


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OfflineProfessorPinHead
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14144052 - 03/18/11 04:18 PM (12 years, 10 months ago)

Nice

:borat:


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Invisiblejokefox
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: ProfessorPinHead]
    #14144066 - 03/18/11 04:20 PM (12 years, 10 months ago)

i still cant get over how beautiful the mycelium is colonizin in the very first pic
reminds me of the fields,


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14153430 - 03/20/11 11:54 AM (12 years, 10 months ago)

updated fruiting pics :smile2:

tub A (3-19)                    middle of day 12                tub B (3-19)

Tub A (3-20)                    middle of day 13                Tub B (3-20)


LW




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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14165256 - 03/22/11 03:19 PM (12 years, 10 months ago)

an update... (middle of day 15 fruiting) almost ready to harvest 1st flush... mmm brains... :zombie:

Tub A



Tub B


:zombie2: BRAINS! :zombie3: :zombie4: NOMNOMNOM! :zombie5: RRR! :zombie6: <-this guy is humpin' the shit outta something fierce!
:zombie7: ZOMBIE FAIL! :zombiedrool:

LW


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14176653 - 03/24/11 03:42 PM (12 years, 10 months ago)

Update... (middle of day 17 fruiting) ready to harvest most of the 1st flush from both tubs...

Tub A (before harvest)                                  (after harvest)

101 wet grams


Tub B (before harvest)                                  (after harvest)

121 wet grams


will harvest the rest of both tubs tonight/tomorrow and update...

LW


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Offlineburdturd
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14176737 - 03/24/11 03:56 PM (12 years, 10 months ago)

:mushroom2::thumbup:  Looking very nice and tasty!


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: burdturd]
    #14182669 - 03/25/11 02:59 PM (12 years, 10 months ago)

1st flush Harvest

Tub A
142 wet grams, another 15 wet grams (not pictured) was harvested a few hours after this...

Tub B
152 wet grams

now, both tubs are in dunk phase...

LW


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OfflineLurkingWizards
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Re: tubs colonizing w/o bag in open room (pics) fruiting update! [Re: LurkingWizards]
    #14294334 - 04/15/11 06:28 AM (12 years, 9 months ago)

Late on the update... computer has been down...

this all happened over 10 days ago...

PICS from Second Flush
Tub A


Tub B


no scales, no dry weight... makes me go crazy :zombie:


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