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mycorasta
Stranger
Registered: 11/12/09
Posts: 63
Last seen: 4 years, 6 months
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Dionized/disstiled water for agar
#14058999 - 03/03/11 09:52 AM (12 years, 10 months ago) |
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Has anyone noticed a difference in using either for making agar. I used deionized water myself because that is what is in the lab i use. Could it affect growth, and does this leave room form more additives?
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Loc: Seattle
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Re: Dionized/disstiled water for agar [Re: mycorasta]
#14059139 - 03/03/11 10:30 AM (12 years, 10 months ago) |
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I used to use all sorts of expensive water purchased in plastic jugs which contribute to the huge waste problem. I switched to tap water years ago and saw no decline in performance. When I moved to my current location, I started using my well water, which is extremely hard and pH 9. It also works very well.
The bottom line is use what is already available in your house. If you're worried about it, boil first, then mix your agar. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
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Loc: Canada
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Re: Dionized/disstiled water for agar [Re: RogerRabbit]
#14059867 - 03/03/11 12:51 PM (12 years, 10 months ago) |
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^ What RR said... I bought distilled water initially for my agar/syringes as well, but quickly just gave up after seeing his advice.
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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ABC
Stranger



Registered: 11/16/08
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Re: Dionized/disstiled water for agar [Re: FalseMaria]
#14060345 - 03/03/11 02:11 PM (12 years, 10 months ago) |
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At college, we use DI water for making prepping agar. At home, I use tap.
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SpawnRun
Morchella esculenta



Registered: 11/12/10
Posts: 301
Loc: Oregon
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Re: Dionized/disstiled water for agar [Re: RogerRabbit]
#14071000 - 03/05/11 01:35 PM (12 years, 10 months ago) |
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Quote:
RogerRabbit said:
The bottom line is use what is already available in your house. If you're worried about it, boil first, then mix your agar. RR
I concur.
-------------------- Clayton Wiseman
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shroomie_glen
RedHotPussyLiquor



Registered: 03/01/06
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Re: Dionized/disstiled water for agar [Re: SpawnRun]
#14082046 - 03/07/11 04:09 PM (12 years, 10 months ago) |
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In my experience, the dissolved minerals in tap/well water can ONLY benefit the diversity of the nutrient profile of your agar.
HOWEVER, I must include a tad of anecdotal evidence.....
After a snow/icing up here in Ohio, they heavily salt the roads, and this leeches into our well at times. THAT is the ONLY time I have ever noticed any decrease of mycological performance from just normal tap water. (I would think due to the overwhelming amt. of salts present.)
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No. No, man. Shit, no man. I believe you'd get your ass kicked sayin' somethin' like that man.
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NolaNomad
Practicing Mycophile



Registered: 05/03/09
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Re: Dionized/disstiled water for agar [Re: RogerRabbit]
#14710225 - 07/03/11 08:37 AM (12 years, 6 months ago) |
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This post came up on a search and helped out tremendously. Thanks again, man.
-------------------- Isolate. Cultivate. Emancipate. Disclaimer- The Author of this post assumes no responsibility for any information, factual or not, that he has posted herein. He does not grow mushrooms. Those pictures are fake. The information is all lies. You heard me. I'm a liar. I only take drugs prescribed to me.
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johnm214


Registered: 05/31/07
Posts: 17,582
Loc: Americas
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Re: Dionized/disstiled water for agar [Re: ABC]
#14713057 - 07/03/11 09:55 PM (12 years, 6 months ago) |
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Quote:
ABC said: At college, we use DI water for making prepping agar. At home, I use tap.
Do you know why? I would guess its simply for repeatability as they can exclude water variability from being a signifigant factor in the agar's charecteristics. I would think if anything it would help when making media, but I guess it depends on what your after in your agar.
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Hamsta
Confoosed


Registered: 06/10/11
Posts: 5
Last seen: 12 years, 6 months
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Re: Dionized/disstiled water for agar [Re: johnm214]
#14723963 - 07/06/11 02:18 AM (12 years, 6 months ago) |
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Quote:
johnm214 said:
Quote:
ABC said: At college, we use DI water for making prepping agar. At home, I use tap.
Do you know why? I would guess its simply for repeatability as they can exclude water variability from being a signifigant factor in the agar's charecteristics. I would think if anything it would help when making media, but I guess it depends on what your after in your agar.
In chem labs, it's to reduce/prevent contamination *the more chemistry related kind of contams, not the bacterial kind* Would suck if trace amounts of minerals in the tap screwed over a carefully prepared experiment. In bio labs, DI water to make defined medium & since tap water tends to be chlorinated & have trace minerals & unknown byproducts, could affect results when growing bacteria.
Who knows what these might do to your experiment. o_o' http://en.wikipedia.org/wiki/Disinfection_byproduct
-------------------- Hamsters: Warming hearts & changing outlooks since the dawn of time. Solving Ennui said: its the cutest thing ive ever seen... my life is changed forever yes, the cute little whatever it is has warmed my heart and changed my outlook on the world, yes the mexicans were indians bred out by the spanish, and yes their genetics are inferior to mine and they shouldnt be allowed to reproduce, but i love them, because the cute little fur ball wants me to.
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